CHEWING GUM CONTAINING PROPOLIS WAX FROM APIS MELLIFERA BEES FOR PREVENTION OF DENTAL CARIES DISEASE

  • SRI ANGKY SOEKANTO Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • ASTRID LEVINA Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
  • EKA NURIN SHARFINA IRIANTO Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.
  • ACHMAD HUDA FAUZI ADZIMA Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.
  • HERI HERMANSYAH Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.
  • ANONDHO WIJANARKO Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.
  • MUHAMAD SAHLAN Research Center for Biomedical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, Indonesia.

Abstract

Objective: Dental caries is caused by demineralization of the tooth surface by organic acids as a result of metabolism of cariogenic bacteria, especially
Streptococcus mutans. Chewing gum has been proven to help prevent dental caries formation by stimulating salivary flow and thus reducing the
availability of nutrients for cariogenic bacteria.
Methods: In this study, sugar-free chewing gum was formulated using latex from the jelutong tree (Dyera costuata) with the addition of propolis wax
(1–5%) as an antibacterial agent to inhibit the activity of S. mutans and prevent biofilm formation, which is an early disease stage of dental caries.
Characterization of jelutong latex using differential scanning calorimetry and thermogravimetric analysis shows that the latex has glass transition
temperatures of −22.46°C and 33.86°C, and it contains volatile (14.96%), polymer (84.43%), organic (0.36%), and inorganic (0.25%) components.
The propolis wax used in this formulation mostly contains resin and wax and is a purification by-product of raw propolis obtained from Apis mellifera.
Results: Flavonoids acting as antibacterial compounds are found in propolis, and presumably are also found in propolis wax; thus, probably this
compound also has the antibacterial properties of propolis. The in vitro testing showed that 5% propolis wax in sugar-free chewing gum can prevent
the formation of dental caries by inhibiting biofilm formation with up to 80% more effectiveness relative to negative controls (without chewing gum).
Conclusion: Propolis wax chewing gum can inhibit the formation of dental caries disease.

Keywords: Biofilm, Chewing gum, Formulation Streptococcus mutans, Wax propolis

References

1. Health Research and Development Agency. Basic Health Research
(RISKESDAS) 2013. National Report 2013; 1-384.
2. Forssten SD, Bjorklund M, Ouwehand AC. Streptococcus mutans,
caries and simulation models. Nutrients 2010;2:290-8.
3. Alanzi A, Soderling E, Varghese A, Honkala E. Xylitol chewing
gums on the market: Do they prevent caries? Oral Health Prev Dent
2016;14:459-66.
4. Veloz JJ, Saavedra N, Alvear M, Zambrano T, Barrientos L, Salazar LA,
et al. Polyphenol-rich extract from propolis reduces the expression and
activity of Streptococcus mutans glucosyltransferases at subinhibitory
concentrations. Biomed Res Int 2016;2016:4302706.
5. Gum Base Co. Gum Base. Gum Base Co; 2009. Available from:
http://www.gumbase.com/on-line/Home/Confectionery/Products/
GUMBASE.html. [Last retrieved on 2014 Apr 15].
6. Nield-Gehrig J, Willmann DE. Dental Plaque Biofilms. Foundations of
Periodontics for the Dental Hygienist; @ 2003 Lippincott Williams and
Wilkins. p. 67-73. Available from: http://www.lww.com.
7. Koo H, Rosalen PL, Cury JA, Park YK, Bowen WH. Effects of
compounds found in propolis on Streptococcus mutans growth
and on glucosyltransferase activity. Antimicrob Agents Chemother
2002;46:1302-9.
8. Huang R, Li M, Gregory RL. Bacterial interactions in dental biofilm.
Virulence 2011;2:435-44.
9. Soekanto SA, Duhita GC, Bachtiar EW, Sahlan M. The effect of casein
phosphopeptide-amorphous calcium phosphate-propolis chewing gum
on calcium and phosphate ion levels in caries-free subject’s saliva
and Streptococcus mutans biofilm formation. Asian J Pharm Clin Res
2017;S:174-6.
10. Hasnamudhia F, Bachtiar EW, Sahlan M, Soekanto SA. The effect of
CPP-ACP-propolis chewing gum on calcium and phosphate ion release
on caries-active subjects’ saliva and the formation of Streptococcus
mutans biofilm. J Phys 2017;884:12137.
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How to Cite
SOEKANTO, S. A., LEVINA, A., SHARFINA IRIANTO, E. N., ADZIMA, A. H. F., HERMANSYAH, H., WIJANARKO, A., & SAHLAN, M. (2019). CHEWING GUM CONTAINING PROPOLIS WAX FROM APIS MELLIFERA BEES FOR PREVENTION OF DENTAL CARIES DISEASE. International Journal of Applied Pharmaceutics, 11(1), 141-145. https://doi.org/10.22159/ijap.2019.v11s1.15941
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