THE EFFECT OF ANTICOAGULANT TYPES ON THE IN VITRO ANALYSIS OF CLOPIDOGREL IN HUMAN PLASMA USING LIQUID CHROMATOGRAPHY TANDEM-MASS SPECTROMETRY

  • Yahdiana Harahap Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Anisa Maulidina Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Delly Ramadon Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia

Abstract

Objective: The aim of this study was to optimize and validate a plasma clopidogrel analysis method using liquid chromatography tandem-mass
spectrometry.
Methods: Plasma samples were analyzed using a BEH C18 column (1.7 μm; 100 mm×2.1 mm), the mobile phase was 0.1% formic acid in acetonitrile
(30:70, v/v). The flow rate was 0.2 mL/min, with a column temperature set to 35°C, an injection volume of 5 μL, an analysis time of 4 min, and
irbesartan as the internal standard. Aliquots were obtained by liquid-liquid extraction using ammonium acetate and diethyl ether. The stability and
peak area ratio of the respective plasma area responses were evaluated using ANOVA.
Results: No significant differences (p>0.05) were observed between anticoagulants regarding analyte stability. However, the peak area ratio
showed significant differences (p<0.05) between the anticoagulants. The accuracy and precision of the analysis with citrate, heparin, and
ethylenediaminetetraacetic acid (EDTA) plasma met the quality requirements, and a linear calibration curve was created with concentrations ranging
from 0.02 to 5.0 ng/mL.
Conclusion: The results showed that improved analysis of clopidogrel was achieved using citrate or heparin plasma compared with EDTA plasma.

Keywords: Citrate, Clopidogrel, Ethylenediaminetetraacetic acid, Heparin, Liquid chromatography tandem-mass spectrometry.

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How to Cite
Harahap, Y., Maulidina, A., & Ramadon, D. (2018). THE EFFECT OF ANTICOAGULANT TYPES ON THE IN VITRO ANALYSIS OF CLOPIDOGREL IN HUMAN PLASMA USING LIQUID CHROMATOGRAPHY TANDEM-MASS SPECTROMETRY. International Journal of Applied Pharmaceutics, 10(1), 392-395. https://doi.org/10.22159/ijap.2018.v10s1.87
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