THE EFFECT OF ANTICOAGULANT TYPES ON THE IN VITRO ANALYSIS OF CLOPIDOGREL IN HUMAN PLASMA USING LIQUID CHROMATOGRAPHY TANDEM-MASS SPECTROMETRY


Yahdiana Harahap, Anisa Maulidina, Delly Ramadon

Abstract


Objective: The aim of this study was to optimize and validate a plasma clopidogrel analysis method using liquid chromatography tandem-mass
spectrometry.
Methods: Plasma samples were analyzed using a BEH C18 column (1.7 μm; 100 mm×2.1 mm), the mobile phase was 0.1% formic acid in acetonitrile
(30:70, v/v). The flow rate was 0.2 mL/min, with a column temperature set to 35°C, an injection volume of 5 μL, an analysis time of 4 min, and
irbesartan as the internal standard. Aliquots were obtained by liquid-liquid extraction using ammonium acetate and diethyl ether. The stability and
peak area ratio of the respective plasma area responses were evaluated using ANOVA.
Results: No significant differences (p>0.05) were observed between anticoagulants regarding analyte stability. However, the peak area ratio
showed significant differences (p<0.05) between the anticoagulants. The accuracy and precision of the analysis with citrate, heparin, and
ethylenediaminetetraacetic acid (EDTA) plasma met the quality requirements, and a linear calibration curve was created with concentrations ranging
from 0.02 to 5.0 ng/mL.
Conclusion: The results showed that improved analysis of clopidogrel was achieved using citrate or heparin plasma compared with EDTA plasma.


Keywords


Citrate, Clopidogrel, Ethylenediaminetetraacetic acid, Heparin, Liquid chromatography tandem-mass spectrometry.

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About this article

Title

THE EFFECT OF ANTICOAGULANT TYPES ON THE IN VITRO ANALYSIS OF CLOPIDOGREL IN HUMAN PLASMA USING LIQUID CHROMATOGRAPHY TANDEM-MASS SPECTROMETRY

Keywords

Citrate, Clopidogrel, Ethylenediaminetetraacetic acid, Heparin, Liquid chromatography tandem-mass spectrometry.

DOI

10.22159/ijap.2018.v10s1.87

Date

20-12-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 10, Special Issue 1 (Dec.), 2018 Page: 392-395

Online ISSN

0975-7058

Authors & Affiliations

Yahdiana Harahap
Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia

Anisa Maulidina
Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia

Delly Ramadon
Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia


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