FORMULATION, STABILITY TFORMULATION, STABILITY TEST AND IN VITRO PENETRATION STUDY OF TRANSETHOSOMAL GEL CONTAINING GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAVES EXTRACTEST AND IN VITRO PENETRATION STUDY OF TRANSETHOSOMAL GEL CONTAINING GREEN TEA(CAMELLIA SINENSIS L. KUNTZE) LEAVES EXTRACT
Objective: The aim of this study was to increase penetration of epigallocatechin gallate (EGCG) from the extract using transethosomal gel.
Methods: Transethosomes (TE) formulae were made using thin layer hydration method with different concentration of green tea extract which was equivalent to 1% (F1), 1.5% (F2), and 2% (F3) of EGCG. F1 was the chosen formula to be incorporated into a gel as a transethosomal gel (TEG). A gel containing green tea extract was also made as a control called as non-transethosomal gel (NTEG). A stability test and in vitro penetration study of gels using Franz diffusion cell were performed.
Results: F1 was the chosen formula because it had a spherical shape, a particle size of 112.14Â±2.19 nm, PDI of 0.166Â±0.03, a zeta potential of-52.05Â±1.34 mV, and %EE of 58.06Â±0.08%. Stability test results showed that TEG more stable than NTEG. The amount of EGCG penetrated from TEG and NTEG were 1391.16Â±34.89 Âµg/cm2 and 485.29Â±14.49 Âµg/cm2, respectively (p<0.05). The lag time for TEG was around 0.99Â±0.2 h, while NTEG was 8.69Â±0.2 h (p<0.05).
Conclusion: It can be concluded that transethosomes can improve gel stability and increase the amount of EGCG penetrated through the skin.Â
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