FORMULATION OF A CREAM CONTAINING ETHOSOMAL GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAF EXTRACTS FOR IMPROVED DERMAL PENETRATION

  • Effionora Anwar Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Jeanetha Inees Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Delly Ramadon Department of Pharmaceutical Technology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.

Abstract

Objective: This study aimed to formulate the epigallocatechin gallate (EGCG) from green tea into ethosomes and measure the resulting increases in
skin penetration using a rat model.
Methods: Ethosomes were formulated using ethanol concentrations of 25% (F1), 30% (F2), and 35% (F3), and those with favorable characteristics
were then incorporated into a cream for the determination of penetration into Franz diffusion cells.
Results: We showed that the formulation F3 had the best spherical morphology, a Z-average value of 73.01 nm, a polydispersity index of 0.26, a
zeta potential of −47.77±3.93 mV, and the highest percentage of drug entrapment (49.46%±0.62%) compared with the other formulas. The total
cumulative EGCG penetration from the resulting ethosomal cream was 905.75±49.47 μg/cm2, whereas that from the cream containing green tea leaf
extract was only 413.92±52.83 μg/cm2.
Conclusion: These data indicate a higher penetration of EGCG from ethosomal green tea cream than from cream containing non-ethosomal green tea
extract.

Keywords: Epigallocatechin gallate, Ethosomal green tea leaf extract, Topical cream, Dermal penetration.

References

1. Namita P, Mukesh R, Vijay KJ. Camellia sinensis (Green Tea):
A review. Global J Pharm 2012;6:52-9.
2. Sharangi AB. Medicinal and therapeutic potentialities of tea (Camellia
sinensis L.) – A review. Food Res Int 2009;42:529-35.
3. Mohan M, Jeevanandan G, Mithun RS. The role of greentea in oral
health – A review. Asian J Pharm Clin Res 2018;11:1-3.
4. Anand J, Gautam P, Rai N. Comparative study of antibacterial and
anti-proliferative potential of green tea from different geographical
locations in India. Asian J Pharm Clin Res 2015;8:253-8.
5. Du G, Zhang Z, Wen X, Yu C, Calway T, Yuan C, et al. Epigallocatechin
gallate (EGCG) is the most effective cancer chemopreventive
polyphenol in green tea. Nutrients 2012;8:1679-91.
6. Anand J, Upadhyaya B, Rawat P, Rai N. Biochemical characterization
and pharmacognostic evaluation of purified catechins in green tea
(Camellia sinensis) cultivars of India. 3 Biotech 2014;5:285-94.
7. Batchelder RJ, Calder RJ, Thomas CP, Heard CM. In the vitro
transdermal delivery of the major catechins and caffeine from an extract
of Camellia sinensis. Int J Pharm 2004;283:45-51.
8. Meireles D, Hunstein W. Epigallocatechin-3 gallate (EGCG) for clinical
trials: More pitfalls than promise. Int J Mol Sci 2011;12:5592-603.
9. Sharma A, Saini S, Rana AC. Transdermal drug delivery system:
A review. Int J Res Pharm Biomed Sci 2013;4:286-92.
10. Shelke S, Shahi S, Kale S, Patil V, Deshpande D. Ethosomes : A novel
deformable carrier. World J Pharm Sci 2015;3:1830-9.
11. Anwar E, Utami TD, Ramadon D. Transfersomal gel containing green
tea (Camellia sinensis L. kuntze) leaves extract: Increasing in vitro
penetration. Asian J Pharm Clin Res 2017;10:294-8.
12. Prausnitz MR, Langer R. Transdermal drug delivery. Nat Biotechnol
2009;26:1261-8.
13. Rakesh R, Anoop KR. Ethosomes for transdermal and topical drug
delivery. Int J Pharm Pharm Sci 2012;4:17-24.
14. Patel AR, Velikov KP. Non-covalent interactions of green tea
polyphenols: Source of novel colloidal structures. Nova Biomedical
2013;2013:151-166.
15. Touitou E, Dayan N, Bergelson L, Godin B, Eliaz M. Ethosomes - Novel
vesicular carriers for enhanced delivery: Characterization and skin
penetration properties. J Control Release 2000;65:403-18.
16. Verma P, Pathak K. Therapeutic and cosmeceutical potential of
ethosomes: An overview. J Adv Pharm Technol Res 2010;1:274.
17. Abdulbaqi IM, Darwis Y, Khan NA, Assi RA, Khan AA. Ethosomal
nanocarriers: The impact of constituents and formulation techniques on
ethosomal properties, in vivo studies, and clinical trials. Int J Nanomed
2016;11:2279-304.
18. Baumann L, Baumann L. Cosmetic Dermatology. 1st ed. New York:
McGraw-Hill Medical; 2009.
19. Maheshwari RG, Tekade RK, Sharma PA, Darwhekar G, Tyagi A,
Patel RP, et al. Ethosomes and ultradeformable liposomes for
transdermal delivery of clotrimazole: A comparative assessment. Saudi
Pharm J 2012;20:161-70.
20. Fangueiro JF, Parra A, Silva AM, Egea MA, Souto EB, Garcia ML, et al.
Validation of a high performance liquid chromatography method for the
stabilization of epigallocatechin gallate. Int J Pharm 2014;475:181-90.
21. Pantone I Color Book. Simulations of Pantone Matching System
Colors. New Jersey: Pantone I Color Book; 2004.
Statistics
40 Views | 54 Downloads
How to Cite
Anwar, E., Inees, J., & Ramadon, D. (2018). FORMULATION OF A CREAM CONTAINING ETHOSOMAL GREEN TEA (CAMELLIA SINENSIS L. KUNTZE) LEAF EXTRACTS FOR IMPROVED DERMAL PENETRATION. International Journal of Applied Pharmaceutics, 10(1), 221-224. https://doi.org/10.22159/ijap.2018.v10s1.49
Section
Original Article(s)