FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS

  • Dewi Kumala Putri Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Berna Elya Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Nuraini Puspitasari Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.

Abstract

Objective: To assess the antioxidant activity from another part of the plant, in this study, leaf extracts in n-hexane were fractionated.
Methods: Ten fractions were obtained and tested in vitro for antioxidant activity using two methods, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and ferric
reducing antioxidant power (FRAP), to identify the most active fraction.
Results: The IC50 of the most active fraction was 36.24 μg/mL using the DPPH method, and the EC50 was 39.54 μg/mL using the FRAP method. The
most active fraction was also shown to contain terpenoids.
Conclusion: The most active fraction of an n-hexane extract of the leaves of Gacinia bancana Miq., which was tested by both DPPH and FRAP methods
had antioxidant activities with IC50 and EC50 values of 36.2482 μg/mL and 39.5442 μg/mL, respectively. Phytochemical screening showed that active
fraction contains terpenoids.

Keywords: 1, 1-diphenyl-2-picrilhydrazyl, Antioxidant, Ferric reducing antioxidant power, Gacinia bancana Miq.

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Putri, D. K., Elya, B., & Puspitasari, N. (2018). FRACTIONATION OF THE N-HEXANE EXTRACT OF GARCINIA BANCANA MIQ. (MANGGIS HUTAN) LEAVES AND ITS ANTIOXIDANT ACTIVITY BASED ON 1,1-DIPHENYL-2- PICRYLHYDRAZYL AND FERRIC REDUCING ANTIOXIDANT POWER ASSAYS. International Journal of Applied Pharmaceutics, 10(1), 407-411. https://doi.org/10.22159/ijap.2018.v10s1.90
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