DETERMINATION OF SPECIFIC AND NON-SPECIFIC PARAMETERS OF THE SIMPLICIA AND ETHANOL EXTRACT OF SANGKETAN (ACHYRANTHES ASPERA L.) LEAVES
Objective: With recent advancements in traditional medicine, there is an increasing need for quality assurance assessments using standardized
processes. In this study, we aimed to determine the specific and non-specific parameters of the Simplicia and 70% ethanol extract of Achyranthes
aspera leaves from three different regions.
Methods: Samples were extracted using a maceration method with 70% ethanol as the solvent. The tests for the specific and non-specific parameters
were based on the Herbal Pharmacopoeia of Indonesia.
Results: Our results of the specific parameters of the Simplicia showed that the ethanol-soluble extract contained 5.83–9.36% and the water-soluble
extract contained 10.25–15.44%. The chromatogram pattern used β-sitosterol as the standard, and the total phenolic content was 0.93–1.15 mg gallic
acid equivalent (GAE)/g Simplicia. The results of the non-specific parameters of Simplicia showed a loss on drying of 15.25–15.91%, a total ash content
of 14.58–20.79%, and an acid-insoluble ash value of 1.75–2.19%.
Conclusion: Our results of the specific parameters of A. aspera extract showed a total phenolic content of 6.49–7.68% mg GAE/g extract. Our results
of the non-specific parameters of the extract showed a total ash value of 13.18–14.52%, an acid-insoluble ash value of 0.14–0.29%, and a moisture
content of 10.42–11.16%.
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