• HARMITA HARMITA Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • ISKANDARSYAH Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia
  • FRIAINI ZAHRA MURTI Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia


Objective: Glutathione is an important antioxidant compound that is added to various cosmetic preparations. This study compared the stability,
antioxidant activity, and penetration of glutathione creams formulated with and without transfersome, a commonly used carrier system.
Methods: The particle size of the transfersome was 55.65 nm, with a polydispersity index of 0.398 and an entrapment efficiency of 66.22%. During
cycling and centrifugal testing, the creams (with and without transfersome) did not change color or demonstrate phase separation. Chemical stability
analyses of the products were performed using high-performance liquid chromatography.
Results: The remaining glutathione content in the transfersome cream was 83.44%, while that of the non-transfersome cream was 47.92%. In the
penetration test using Franz diffusion cells, the transfersome cream demonstrated a cumulative penetration of 4474.44 μg/cm2, with a cumulative
concentration percentage of 39.60% and a flux of 510.38 μg/cm−2h−1. In contrast, the non-transfersome cream demonstrated a cumulative penetration
amount of 2793.80 μg/cm2, with a cumulative concentration percentage of 24.73% and a flux of 340.12 μg/cm−2h−1. In addition, the IC50 value of the
transfersome cream preparation was 11.89 μg/mL, while that of the non-transfersome preparation was 15.57 μg/mL.
Conclusion: Our findings indicate that the use of transfersome increases the stability and penetration of glutathione in cream preparations.

Keywords: Glutathione, Transfersome, Cream, High-performance liquid chromatography, Optimization, Stability test, Antioxidant activity test, Penetration test


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How to Cite
HARMITA, H., ISKANDARSYAH, & MURTI, F. Z. (2020). EFFECT OF TRANSFERSOME ON THE STABILITY AND ANTIOXIDANT ACTIVITY OF GLUTATHIONE IN ANTIAGING CREAMS. International Journal of Applied Pharmaceutics, 12(1), 149-155. https://doi.org/10.22159/ijap.2020.v12s1.FF033
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