INHIBITORY EFFECTS OF SANGKETAN (ACHYRANTHES ASPERA L.) ROOT EXTRACTS ON ARGINASE ACTIVITY
Objective: Achyranthes aspera, commonly called Sangketan in Indonesian, is a wild plant used as traditional medicine. The roots of Sangketan can be
used as a wound healer, which is attributed to the involvement of arginine and its metabolites, nitric oxide, which directly influence the wound healing
process. The aim of the present study was to determine the potential of Sangketan root extracts to inhibit arginase activity.
Methods: The roots were extracted using the multistage ultrasound-assisted extraction method using n-hexane, ethyl acetate, and methanol solvents.
Each of the extract associated with the three solvents was tested for arginase inhibition activity using a microplate-based colorimetric method,
followed by the determination of total phenolic compound and total flavonoid concentrations.
Results: The results of the inhibition test of arginase activity based on n-hexane, ethyl acetate, and methanolic extracts were 9.56, 17.58, and 29.77%,
respectively, at concentrations of 100 μg/mL; the total phenolic compound concentrations were 3.91, 4.83, and 11.18 mg GAE/g of sample, respectively,
and the total flavonoid concentrations were 0.29, 0.80, and 0.88 mg QE/g of sample, respectively.
Conclusion: According to the results, Sangketan root extracts had low potency with regard to arginase inhibitory activity.
folk herbal medicinal plant: Achyranthes aspera L. Int J Med Plants
2. Kamalakkannan K, Balakrishnan B. Studies on the effect of antidiabetic
activity of Achyranthes aspera L. On alloxan induced wistar rats. Int J
Pharm Pharm Sci 2015;7:61-4.
3. Bhosale UA, Yegnanarayan R, Pophale P, Somani R. Effect of aqueous
extracts of Achyranthes aspera Linn. On experimental animal model
for inflammation. Anc Sci Life 2012;31:202-6.
4. Gould A, Naidoo C, Candy GP. Arginine Metabolism and Wound
Healing. Vol. 1. Pune: WHSA; 2008. p. 48-50.
5. Steppan J, Nyhan D, Berkowitz DE. Development of novel arginase
inhibitors for therapy of endothelial dysfunction. Front Immunol
6. Kavalukas SL, Uzgare AR, Bivalacqua TJ, Barbul A. Arginase
inhibition promotes wound healing in mice. Surgery 2012;151:287-95.
7. Bordage S, Pham TN, Zedet A, Gugglielmetti AS, Nappey M,
Demougeot C, et al. Investigation of mammal arginase inhibitory
properties of natural ubiquitous polyphenols by using an optimized
colorimetric microplate assay. Planta Med 2017;83:647-53.
8. Cray JA, Bhaganna P, Singhal RS, Patil SV, Saha D, Chakraborty R, et al.
Chaotropic and hydrophobic stress mechanism of antifungal substances.
In: Dehne HW, Deising HB, Fraaije B, Gisi U, Hermann D, Mehl A,
et al., editors. Modern Fungicides and Antifungal Compounds. Vol. 7.
Braunschweig: Deutsche Phytomedizinische Gesellschaft; 2014. p. 73-80.
9. Girard-Thernier C, Pham TN, Demougeot C. The promise of plantderived
substances as inhibitors of arginase. Mini Rev Med Chem
10. Zalsabela TL, Elya B, Noviani A. Arginase inhibition activity of stem
bark extract of Caesalpinia pulcherrima. J Young Pharm 2018;10:111-3.
11. Bobo-García G, Davidov-Pardo G, Arroqui C, Vírseda P, Marín-
Arroyo MR, Navarro M. Intra-laboratory validation of microplate
methods for total phenolic content and antioxidant activity
on polyphenolic extracts, and comparison with conventional
spectrophotometric methods. J Sci Food Agric 2015;95:204-9.
12. Medina-Torres N, Ayora-Talavera T, Espinosa-Andrews H, Sánchez-
Contreras A, Pacheco N. Ultrasound assisted extraction for the recovery
of phenolic compounds from vegetable sources. Agronomy 2017;7:47.
13. Adawiah A, Sukandar D, Muawanah A. Antioxidant activity and
content of bioactive components of namnam juice. J Kimia Valensi
14. Ministry of Health of the Republic of Indonesia. Indonesian Herbal
Pharmacopoeia (Supplement III). Vol. 1. Jakarta: Ministry of Health of
the Republic of Indonesia; 2013.
15. Singh H, Dixit A, Sharma RA, Sharma A. Comparative evaluation of
total phenolic content, total flavonoid content and dpph free radical
scavenging activity of different plant parts of Vitex negundo L. Int J
Pharm Pharm Sci 2014;7:144-7.
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