POTENTIAL ANTIDIABETIC ACTIVITIES OF FRACTIONS FROM PURIFIED EXTRACT OF LAWSONIA INERMIS LEAVES IN ALLOXAN–INDUCED DIABETIC MICE

Authors

  • MUS IFAYA Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Departemen of Pharmacy, Faculty of Science and Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi Indonesia
  • IDA MUSFIROH Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran
  • SAHIDIN Faculty of Pharmacy, Universitas Halu Oleo Kendari, Southeast Sulawesi Indonesia
  • YASMIWAR SUSILAWATI Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran
  • GOFARANA WILAR Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran
  • SYAWAL ABDURRAHMAN Departement of Medical Laboratory Technology, Mandala Waluya Kendari High School of Health Science, Southeast Sulawesi Indonesia

DOI:

https://doi.org/10.22159/ijap.2021.v13s4.43824

Keywords:

L. inermis, Fractions, Anti-diabetic, Liquid chromatography-mass spectrometry

Abstract

Objective: This research was conducted to determine the potential antidiabetic activity fractions of purified extract Lawsonia inermis leaves in mice (Mus musculus) and identification of the compound.

Methods: The method included maceration, purification using ethanol and distilled water was followed by liquid-liquid extraction using ethyl acetate and magnesium sulfate as drying agents. Furthermore, the extract was analyzed using thin layer chromatography (TLC) for testing the purified extract. Fractionation using vacuum liquid chromatography, antidiabetic activity test of fractions at dose 100 mg/kgBW with alloxan induced and compound identification by Liquid Chromatography-Mass Spectrometry (LC-MS/MS) using HPLC connected to a Q-TOF spectrometer equipped with an ESI source, with Phenomenon column C8, and methanol with 0.3% formic acid as solvent.

Results: The results showed that from the purification step of L. inermis leaves by vacuum liquid chromatography method, 7 fractions were obtained, i.e. A-G fractions. While the antidiabetic activity of fractions shown by decreasing blood sugar level in mice on the 15th day were 64, 75, 73, 73, 57, 45 and 67%, respectively. The identified compounds from each fraction were the ester groups namely 12-hydroxy-methyl abietate, 9,12-octadecadienoic acid (Z,Z)-(2,2-dimethyl-1,3-dioxolan-4-yl)methyl ester, dehydromorroniaglycone, and (E)-hexadecyl-ferulate; the steroid group namely siraitic acid E; phenylpropanoid groups namely umbelliferone and bletilol C, and the alkaloid groups namely moupinamide and valine.

Conclusion: L. inermis leaves had activity in lowering blood sugar levels. LC-MS/MS analysis revealed the presence of ester groups, steroid groups, phenylpropanoid groups and alkaloid groups. The presence of these compounds mostly contribute to antidiabetic activity.

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Published

11-12-2021

How to Cite

IFAYA, M., MUSFIROH, I., SAHIDIN, SUSILAWATI, Y., WILAR, G., & ABDURRAHMAN, S. (2021). POTENTIAL ANTIDIABETIC ACTIVITIES OF FRACTIONS FROM PURIFIED EXTRACT OF LAWSONIA INERMIS LEAVES IN ALLOXAN–INDUCED DIABETIC MICE. International Journal of Applied Pharmaceutics, 13(4), 89–94. https://doi.org/10.22159/ijap.2021.v13s4.43824

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