METHOD DEVELOPMENT AND VALIDATION FOR ANALYSIS OF DEOXYARBUTININ ANHYDROUS EMULSION SYSTEM USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY

  • MUCHTARIDI MUCHTARIDI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia http://orcid.org/0000-0002-6156-8025
  • IDA MUSFIROH Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia
  • AHMAD FAUZI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy Universitas Padjadjaran, Sumedang, Indonesia

Abstract

Objective: The aim of this study is to develop a simple, precise and accurate analytical method of deoxyarbutin in anhydrous emulsion system preparation.


Methods: The analysis was conducted using high-performance liquid chromatography (HPLC). Chromatographic analysis was carried out using a reversed phase-C18 column. The mobile consists of two phases methanol and water (60: 40 v/v) at a flow rate of 1.0 ml/min. The determinations were performed using UV detector set at 225 nm. All validation procedures were added with hydroquinone as an internal standard.


Results: The method showed coefficient correlation is 0.9978, relative standard deviation (RSD) smaller than 2%, Limit of Detection (LOD) and Limit of Quantitation (LOQ) are 0.599 µg/ml and 1.817 µg/ml respectively. The total amount deoxyarbutin in anhydrous emulsion preparation is 1.964+0.02 % with 98% recovery percentage.


Conclusion: The developed HPLC analytical method meets the validation criteria made by International Conference on Harmonisation (ICH).

Keywords: Analytical method validation, Anhydrous emulsion, Deoxyarbutin
Statistics
43 Views | 25 Downloads
How to Cite
MUCHTARIDI, M., MUSFIROH, I., & FAUZI, A. (2019). METHOD DEVELOPMENT AND VALIDATION FOR ANALYSIS OF DEOXYARBUTININ ANHYDROUS EMULSION SYSTEM USING HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY. International Journal of Applied Pharmaceutics, 11(2), 172-175. https://doi.org/10.22159/ijap.2019v11i2.30461
Section
Original Article(s)