ANALYTICAL METHOD DEVELOPMENT AND VALIDATION FOR THE DETERMINATION OF CAFFEINE IN GREEN COFFEE BEANS (COFFEA ARABICA L.) FROM THREE DISTRICTS OF WEST JAVA, INDONESIA BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
Objective: To develop and validate a simple, accurate, and precise HPLC method for the determination of caffeine in green coffee beans (Coffea arabica L.) from three districts of West Java, Indonesia.
Methods: The analytical method was conducted using Enduro C-18 (250 x 4.6 mm) column with methanol: water (37: 63) as a mobile phase, the flow rate was 1.0 ml/min, and the detector wavelength was set at 274 nm. The selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy, precision, and system suitability testing were evaluated as the parameters of validation.
Results: The retention time of caffeine was 6.36 min. % RSD for precision was 0.192. The linearity of the method was obtained using a concentration range of 1-200 ppm with the correlation coefficient of 0.998. The limit of detection was 9 ppm and the limit of quantitation was 28 ppm. The accuracy was in between 90.723%-102.853%. Caffeine levels from Garut, Pangalengan, and Tasikmalaya were 1.454 Â± 0.004%, 1.574 Â± 0.082%, and 2.280 Â± 0.004%.
Conclusion: The proposed HPLC method meets the acceptance criteria of validation parameters and can be applied for routine analysis.
2. Wanyika HN, Gatebe EG, Gitu LM, Ngumba EK, Maritim CW. Determination of caffeine content of tea and instant coffee brands found in the Kenyan market. Afr J Food Sci 2010;4:353â€“8.
3. Burke TM, Markwald RR, McHill AW, Chinoy ED, Snider JA, Bessman SC, et al. Effects of caffeine on the human circadian clock in vivo and in vitro. Sci Transl Med 2015;7:1-9.
4. Islam MDR, Alencar MVOB, Mata AMOF, Paz MFCJ, Matos LA, Sousa JMDC, et al. Coffee: a health fuel-blot popular drinking. Int J Pharm Pharm Sci 2016;6:1-7.
5. Al Ghali RM, Al Shaibi H, Al Majed H, Haroun D. Caffeine consumption among Zayed University students in Dubai, United Arab Emirates: a cross-sectional study. Arab J Nutr Exerc 2016;1:131-41.
6. Alomar MJ. Evaluation of caffeine consumption and effect during pregnancy among women in the UAE. Int J Pharm Pharm Sci 2016;6:101-3.
7. Hartono. Coffee production of Indonesia is the biggest three in the world. [Internet]. Jakarta, Indonesia: Ministry of Industry of Indonesia; 2013. Available from: http://www.kemenperin. go.id/artikel/6611/Produksi-Kopi-Nusantara-Ketiga-Terbesar-Di-Dunia. [Last accessed on 16 Jul 2018]
8. Weinberg BA, Bealer BK. The world of caffeine: the scientist and culture of the worldâ€™s most popular drug. New York: Routledge; 2001.
9. Clifford M, Willson K. Coffee: botany, biochemistry, and production of beans and beverage. Croom Helm; 1985.
10. Sampaio BL, Edrada-Ebel R, Da Costa FB. Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: a model for environmental metabolomics of plants. Sci Rep 2016;6:1-11.
11. Ermer J, Miller JHM. Method validation in pharmaceutical analysis: a guide to best practice. United States: John Wiley and Sons; 2005.
12. International Conference on Harmonization, Q2 Validation of analytical proceduresâ€¯: text and methodology international conference on harmonization of technical requirements for registration of pharmaceutical for human use, ICH Harmonized Tripartite Guideline, Canada; 2005.
13. Snyder LR, Kirkland JJ, Glajch JL. Practical HPLC method development. 2nd ed. New York, United States: Wiley-Interscience; 1997.
14. Atomssa T, Gholap AV. Characterization of caffeine and determination of caffeine in tea leaves using uv-visible spectrometer. Afr J Pure Appl Chem 2011;5:1â€“8.
15. Sharma R, Reddy VK, Prashant G, Ojha V, Kumar N. Antimicrobial and anti-adherence activity of various combinations of coffee-chicory solutions on Streptococcus mutans: an in vitro study. J Oral Maxillofac Pathol 2014;18:201-6.
16. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1999;55:225â€“76.
17. The British Pharmacopoeia Commission, British Pharmacopoeia 2013., [internet] United Kingdom: The British Pharmacopeia Commission; 2013. Available from: http://www.-uspbpep.com/bp2013/data/5420.asp. [Last accessed on 16 Jul 2018].
18. Ali MM, Eisa M, Taha MI, Zakaria BA, Elbashir AA. Determination of caffeine in some sudanese beverages by high-performance liquid chromatography. Pakistan J Nutr 2012; 11:336â€“42.
19. Pokhrel P, Shrestha S, Rijal SK, Rai KP. A simple HPLC method for the determination of caffeine content in tea and coffee. J Food Sci Technol Nepal 2016;9:74-8.
20. Rojo De Camargo MC, Toledo MCF. HPLC determination of caffeine in tea, chocolate products and carbonated beverages. J Sci Food Agric 1999;79:1861â€“4.
21. Fajara BEP, Susanti H. HPLC determination of caffeine in the coffee beverage. IOP Conf Ser: Mater Sci Eng 2017;259:1-6.
22. United Nations Office on Drugs and Crime (UNODC), Guidance for the validation of analytical methodology and calibration of equipment used for testing of illicit drugs in seized materials and biological specimens, United Nations Publication, New York; 2009.
23. Nor Azah MA, Sam YY, Mailina J, Chua LSL. (E)-methyl cinnamate: The major component of essential oils of Alpinia malaccensis var nobilis. J Trop For Sci 2005;17:631â€“3.