4-CHLORO-7-NITROBENZOFURAZAN (NBD-CL) AS PRE AND POST COLUMN DERIVATIZATION REAGENT FOR AMINE GROUPS ANALYSIS USING CHROMATOGRAPHY: A MINI REVIEW

Authors

  • FEBRINA AMELIA SAPUTRI Faculty of Pharmacy, Universitas Indonesia, Jalan Margonda Raya, Depok, Indonesia https://orcid.org/0000-0001-8753-6868
  • RIMADANI PRATIWI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Indonesia

DOI:

https://doi.org/10.22159/ijap.2023v15i1.46461

Keywords:

4-Chloro-7-nitrobenzofurazan, chromatography, pre column derivatization, post column derivatization

Abstract

Derivatization is a modification process to produce a new compound that can be detected using suitable detector in chromatography analysis. Derivatization reagent is needed to chemically modify a compound. One of a derivatization reagent that is widely used as a fluorogenic and chromogenic reagent in amine groups analysis using chromatography is 4-Chloro-7-nitrobenzofurazan (NBD-Cl). NBD-Cl is considerable attraction because it plays a role in pre and post-column derivatizations fused in chromatography for increasing the selectivity and sensitivity. This review provides an overview of many papers that have been presented regarding the application of 4-Chloro-7-nitrobenzofurazan (NBD-Cl) in pre and post-column derivatization in amine groups analysis and its chromatographic condition from the literature between 2000 to 2022.

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Published

21-11-2022

How to Cite

SAPUTRI, F. A., & PRATIWI, R. (2022). 4-CHLORO-7-NITROBENZOFURAZAN (NBD-CL) AS PRE AND POST COLUMN DERIVATIZATION REAGENT FOR AMINE GROUPS ANALYSIS USING CHROMATOGRAPHY: A MINI REVIEW. International Journal of Applied Pharmaceutics, 15(1). https://doi.org/10.22159/ijap.2023v15i1.46461

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