VALIDATION OF ANALYTICAL METHOD OF 2,5-HEXANEDIONE ON URINE BY GAS CHROMATOGRAPHY

  • MUCHTARIDI MUCHTARIDI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21 Jatinangor, West Java, Indonesia, 45363 http://orcid.org/0000-0002-6156-8025
  • Kurnia Megawati Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21 Jatinangor, West Java, Indonesia, 45363
  • Febrina Amelia Saputri Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang Km 21 Jatinangor, West Java, Indonesia, 45363
  • Mulyana Mulyana PT. Prodia OHI International, Jalan Kramat Raya No. 148, Central Jakarta 10430, Indonesia

Abstract

Objective: The purpose of this study was to obtain a valid analytical method for determining the level of 2,5-hexanedione in the urine of oil industry workers.


Methods: Gas Chromatography (GC) was employed to analyze 2,5-hexanedione in the urine. The analysis was done using HP-5 (Crosslinked methyl siloxane) capillary columns 30 m x 0.320 mm long, film thickness 0.25 μm. The temperature of the detector temperature was 300 °C, and the injector temperature was 250 °C. The helium gas flow rate was 2 ml/min. The detector was Flame Ionization Detection (FID). Parameters of system suitability test and validation were obtained.


Results: This study results that the method of analysis 2,5-hexanedione in urine by Gas Chromatography (GC) confirm the requirements of the validation method with a linearity was 0.99963, accuracy was in the range of 99.16% to 114.13%, the precision with % coefficient of variation was 1.65% to 5.16%, % coefficient variation of specificity was 0.027%, limit of detection was 0.054 μg/ml and limit of quantification was 0.18 μg/ml.


Conclusion: The proposed GC method meets the acceptance criteria of validation parameters and can be applied for routine analysis.

Keywords: 2,5-hexanedione, Gas chromatography, Hexane, Validation

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MUCHTARIDI, M., Megawati, K., Saputri, F. A., & Mulyana, M. (2019). VALIDATION OF ANALYTICAL METHOD OF 2,5-HEXANEDIONE ON URINE BY GAS CHROMATOGRAPHY. International Journal of Applied Pharmaceutics, 11(4), 320-324. https://doi.org/10.22159/ijap.2019v11i4.32687
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