THE OPTIMIZATION OF ELUTING CONDITION OF SOLID PHASE EXTRACTION METHOD FOR -MANGOSTIN PURIFICATION IN MANGOSTEEN PERICARP EXTRACT


Febrina Amelia Saputri, Annisa Mayangsari, Muchtaridi Muchtaridi

Abstract


Objective: To obtain the optimum condition of eluent selection for purification of α-mangostin in mangosteen pericarp extract with solid phase extraction (SPE) method.

Methods: The analysis was conducted by SPE cartridge octadecylsilane (ODS)-5, solvents used were methanol for conditioning, aqua bidestilated for washing and three different eluents for eluting: methanol, ethanol and ethyl acetate.

Results: Recovery value for each eluent were 32.4741% for methanol, 46.6130% for ethanol and 33.6383% for ethyl acetate. Comparison of alpha-mangostin level in the mangosteen pericarp extract for each eluent i.e. 14.4578% for methanol, 10.0598% for ethanol, and 14.2898 % for ethyl acetate while compared to the extract without SPE was 21.7934%.

Conclusion: Ethanol had the highest recovery value, compared to methanol and ethyl acetate.


Keywords


α-mangostin, Eluent, Optimization, Solid phase extraction

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About this article

Title

THE OPTIMIZATION OF ELUTING CONDITION OF SOLID PHASE EXTRACTION METHOD FOR -MANGOSTIN PURIFICATION IN MANGOSTEEN PERICARP EXTRACT

Keywords

α-mangostin, Eluent, Optimization, Solid phase extraction

DOI

10.22159/ijap.2018v10i3.25415

Date

08-05-2018

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol 10, Issue 3 (May-Jun), 2018 Page: 112-114

Online ISSN

0975-7058

Authors & Affiliations

Febrina Amelia Saputri
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang km 21 Jatinangor, West Java, Indonesia, 45363
Indonesia

Annisa Mayangsari
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang km 21 Jatinangor, West Java, Indonesia, 45363

Muchtaridi Muchtaridi
Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jl Raya Bandung Sumedang km 21 Jatinangor, West Java, Indonesia, 45363
Indonesia


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