THE OPTIMIZATION OF ELUTING CONDITION OF SOLID PHASE EXTRACTION METHOD FOR - MANGOSTIN PURIFICATION IN MANGOSTEEN PERICARP EXTRACT


Febrina Amelia Saputri, Annisa Mayangsari, Muchtaridi Muchtaridi

Abstract


Objective: To obtain the optimum condition of eluent selection for purification of α-mangostin in mangosteen pericarp extract with solid phase extraction (SPE) method.

Methods: The analysis was conducted by SPE cartridge octadecylsilane (ODS)-5, solvents used were methanol for conditioning, aqua bidestilated for washing and three different eluents for eluting: methanol, ethanol and ethyl acetate.

Results: Recovery value for each eluent were 32.4741% for  methanol, 46.6130%  for ethanol and 33.6383% for ethyl acetate. Comparison of alpha-mangostin level in the mangosteen pericarp extract for each eluent i.e. 14.4578% for methanol, 10.0598%  for ethanol, and 14.2898 % for ethyl acetate while compared to the extract without SPE was 21.7934%.

Conclusion:Ethanol had the highest recovery value, compared to methanol and ethyl acetate.

 


References


Shibata MA, Iinuma M, Morimoto J, Kurose H, Akamatsu K, Okuno Y, et al. Alpha-mangostin extracted from the pericarp of the mangosteen (Garcinia mangostana Linn) reduces tumor growth and lymph node metastasis in an immunocompetent xenograft model of metastatic mammary cancer carrying a p53 mutation. BMC Med 2011;9:69.

Zarena AS, Sankar KU. A study of antioxidant properties from Garcinia mangostana L. pericarp extract. Acta Sci Pol Technol Aliment 2009;8:23-34.

Chen LG, Yang LL, Wang CC. Antiinflammatory activity of mangostins from Garcinia mangostana. Food Chem Toxicol 2008;46:688–93.

Taher M, Susanti D, Rezali MF, Zohri FSA, Ichwan SJA, Alkhamaiseh SI, et al. Apoptosis, antimicrobial and antioxidant activities of phytochemicals from Garcinia Malaccensis Hk. f. Asian Pac J Trop Med 2012;5:136-41.

Andayani R, Wahyuni FS, Wirasti Y, Dachriyanus. Development and validation of RP-HPLC method for quantitative estimation of -mangostin in the rind extract and fractions of Garcinia mangostana L. and their cytotoxic activity on T47D breast cancer cell line. Int J Pharm Pharm Sci 2014;7:174-8.

Jung HA, Su BN, Keller WJ, Mehta RG, Kinghorn D. Antioxidant xanthones from the pericarp of Garcinia mangostana (mangosteen). J Agric Food Chem 2006;54:2077-82.

Maisuthisakul P, Suttajit M, Pongsawatmanit R. Assessment of phenolic content and free radicalscavenging capacity of some Thai indigenous plants. Food Chem 2007;100:1409-18.

Rao TP, Daniel S, Gladis JM. Tailored materials for preconcentration or separation of metals by ion-imprinted polymers for solid-phase extraction (IIP-SPE). Trac-Trend Anal Chemy 2004;23:28-35.

McMaster, Marvin C. 2007. HPLC: A Practical User’s Guide Second Edition. New York: John Wiley & Sons, Inc.78.

Muchtaridi M, Yuliani E, Sopyan I. Application of off-line SPE-HPLC/UV methods in analysis of ofloxacin in human urine (in vitro). Int J Pharm Pharm Sci 2015;8:255-61.

Sopyan I, Irawati W, Indriyati W. A novel of bezafibrate analysis methods in urine (in vitro) using solid phase extraction-high performance liquid chromatography-uv detector. Int J App Pharm 2018;10:94-7.

Muchtaridi, Hasanah AN, Musfiroh I. Solid phase extraction: application on analysis preparation. Yogyakarta: Graha Ilmu; 2015. p. 41-6.

Xiao HB, Krucker M, Albert K, Liang XM. Determination and identification of isoflavonoids in Radix Astragali by matrix solid-phase dispersion extraction and high-performance liquid chromatography with photodiode array and mass spectrometric detection. J Chromatogr A 2004;1032:117-24.

Departemen Kesehatan RI. General standard parameters of medicinal plant extracts. Jakarta : Dirjen POM; 2000.

Muchtaridi M, Suryani D, Qosim WA, Saptarini NM. Quantitative analysis of α-mangostin in mangosteen (Garcinia mangostana L.) pericarp extract from four district of west java by HPLC method. Int J Pharm Pharm Sci 2016;8:232-6.

Muchtaridi M, Puteri NA, Milanda T, Musfiroh I. Validation analysis methods of α-Mangostin, -mangostin, and gartanin mixture in mangosteen (Garcinia mangostana L.) fruit rind extract from west java with HPLC. J. Appl. Pharm. Sci 2017;7:125-30.

Snyder LR, JJ Kirkland, JL Glajch. Practical HPLC Method Development. 2nd Edition. New York: John Willey & Sons, Inc; 1997. p. 119-44, 643-728, 736.

Żwir-Ferenc A, Biziuk M. Solid phase extraction technique–trends, opportunities and applications. Pol J Environ Stud 2006;15:677-90.

Chitchumroonchokchai C, Riedl KM, Suksumrarn S, Clinton SK, Kinghorn AD, Failla ML. Xanthones in mangosteen juice are absorbed and partially conjugated by healthy adults. J Nutr 2012;142:675-80.

Walsh KR, Haak SJ, Bohn T, Tian Q, Schwartz SJ, Failla ML. Isoflavonoid glucosides are deconjugated and absorbed in the small intestine of human subjects with ileostomies. Am J Clin Nutr 2007;85:1050-6.

Seo J, Kim HY, Chung BC, Hong J. Simultaneous determination of anabolic steroids and synthetic hormones in meat by freezing-lipid filtration, solid-phase extraction and gas chromatography–mass spectrometry. J Chromatogr A 2005;1067:303-9.

Nurabadi ML, Indriyati W, Musfiroh I. Analysis of Ferric Acid in Arabica Bean Coffee Extract (Coffea arabica L.) by Solid Phase Extraction-High Performance Liquid Chromatography Method. Jatinangor: Fakultas Farmasi UNPAD; 2015.




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