ISOLATION AND DETERMINATION CARPAINE ALKALOID IN PAPAYA (CARICA PAPAYA L.) LEAF EXTRACT BY THIN-LAYER CHROMATOGRAPHY SCANNER

Authors

  • YASMIWAR SUSILAWATI Herbal Study Center, Faculty of Pharmacy, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia, Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
  • NYI MEKAR SAPTARINI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
  • ZELIKA MEGA RAMADHANIA Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
  • ELI HALIMAH Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia
  • NAILAH NURJIHAN ULFAH Department of Biology Pharmacy, Faculty of Pharmacy, Padjadjaran University, Jalan Raya Bandung-Sumedang KM 21, Jatinangor, West Java, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021.v13s4.43867

Keywords:

Carica papaya, Papaya leaf, Carpaine alkaloid, Validation method, TLC scanner

Abstract

Objective: This research was conducted to isolate the alkaloid carpaine by chromatography method and to determine it quantitatively by Thin Layer Chromatography Scanner.

Methods: Dried leaves were macerated with ethanol 70% and fractionated with dichloromethane. Isolation of carpaine alkaloid from the dichloromethane fraction was carried out by column chromatography and preparative thin-layer chromatography according to the Rf value in Thin Layer Chromatography (TLC) after exposure by Dragendorff reagent.

Results: The content of carpaine alkaloid was 7.5 mg with Rf 0.58 and dichloromethane: methanol (9.2:0.8) as eluent. Validation showed the linearity (R2) 0.9988, the limit of detection(LOD) was 0.05 ppm, the Limit of Quantification (LOQ) was 0.19 ppm, the recovery from 98.93-102.43%, and the % coefficient of variation was 0.16%.

Conclusion: Carpaine alkaloid in papaya leaf extract was 10.52%.

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References

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Published

11-12-2021

How to Cite

SUSILAWATI, Y., SAPTARINI, N. M., RAMADHANIA, Z. M., HALIMAH, E., & ULFAH, N. N. (2021). ISOLATION AND DETERMINATION CARPAINE ALKALOID IN PAPAYA (CARICA PAPAYA L.) LEAF EXTRACT BY THIN-LAYER CHROMATOGRAPHY SCANNER. International Journal of Applied Pharmaceutics, 13(4), 234–238. https://doi.org/10.22159/ijap.2021.v13s4.43867

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