ISOLATION, IDENTIFICATION, AND QUANTIFICATION OF MAJOR FLAVONOID IN LEAVES OF PERESKIA BLEO (KUNTH) DC

Authors

  • NYI MEKAR SAPTARINI Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, West Java, Indonesia, 45363
  • RESMI MUSTARICHIE Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, West Java, Indonesia, 45363
  • IRMA ERIKA HERAWATI Indonesian School of Pharmacy, West Java, Indonesia, 40266
  • GINAYANTI HADISOEBROTO Department of Pharmacy, Faculty of Mathematic and Natural Sciences, Al Ghifari University, West Java, Indonesia, 40293

DOI:

https://doi.org/10.22159/ijap.2022.v14s4.PP19

Keywords:

Catechin, Chromatographic separations, Flavonol, Shifting reagent, Purity

Abstract

Objective: This study aimed to isolate, identify, and quantify major flavonoid in P. bleo leaves from West Java Regency, Indonesia.

Methods: Isolation began with maceration, followed by liquid-liquid extraction and various chromatographic separations. Identification of isolates was carried out using 2D TLC and shifting reagents. Quantification was determined with the colorimetric method.

Results: The results showed that extract, ethyl acetate, and distilled water fractions contain flavonoids. The flavonoid screening showed positive results for flavonols. The vacuum liquid chromatography separated 21 sub-fractions; the 12th and 13th sub-fractions were predicted to contain flavonols.

Conclusion: The novelty of this study was the isolation and identification of the major flavonoid of P. bleo leaves originating from West Java Regency, Indonesia, i.e. catechin. The content of the compound was 3.795±0.096 g QE/ml with a purity of 94.89%.

Downloads

Download data is not yet available.

References

Zareisedehizadeh S, Tan CH, Koh HL. A review of botanical characteristics, traditional usage, chemical components, pharmacological activities, and safety of pereskia bleo (Kunth) DC. Evid Based Complement Alternat Med. 2014;2014:326107. doi: 10.1155/2014/326107, PMID 24987426.

Raskin I, Ribnicky DM, Komarnytsky S, Ilic N, Poulev A, Borisjuk N. Plants and human health in the twenty-first century. Trends Biotechnol. 2002;20(12):522-31. doi: 10.1016/s0167-7799(02)02080-2, PMID 12443874.

Krych J, Gebicka L. Catalase is inhibited by flavonoids. Int J Biol Macromol. 2013;58:148-53. doi: 10.1016/j.ijbiomac. 2013.03.070, PMID 23567286.

Zhang X, Huang H, Zhao X, Lv Q, Sun C, Li X. Effects of flavonoids-rich Chinese bayberry (Myrica rubra Sieb. et Zucc.) pulp extracts on glucose consumption in human HepG2 cells. J Funct Foods. 2015;14:144-53. doi: 10.1016/j.jff.2015.01.030.

Directorate general of pharmacy and medical devices. Indonesian herbal pharmacopoeia. 2nd ed. Jakarta, Indonesia: Indonesian Ministry of Health; 2017. p. 545-7.

Saptarini NM, Wardati Y. Effect of extraction methods on antioxidant activity of papery skin extracts and fractions of maja cipanas onion (Allium cepa L. var. ascalonicum). Scientific World Journal. 2020;2020:3280534. doi: 10.1155/2020/3280534. PMID 32351343.

Harborne JB. Phytochemical methods, a guide to modern techniques of plant analysis. 3rd ed. Berlin: Springer Pvt. Ltd; 1998.

Harborne JB, Mabry TJ. The flavonoids advances in research. London, New York: Chapman & Hall; 1982.

Haile M, Kang WH. Antioxidant activity, total polyphenol, flavonoid and tannin contents of fermented green coffee beans with selected yeasts. Fermentation. 2019;5(1):29-34. doi: 10.3390/fermentation5010029.

D’Andrea G. Quercetin: A flavonol with multifaceted therapeutic applications? Fitoterapia. 2015;106:256-71. doi: 10.1016/j.fitote.2015.09.018, PMID 26393898.

Lakhanpal P, Rai DK. Quercetin: a versatile flavonoid. Internet Journal of Medical Update–E-Journal. 2007;2(2):22-37. doi: 10.4314/ijmu.v2i2.39851.

Song TR, Jiang YM, Liu JP, Wang ZL, Zeng J, Huang ZL. Steroid withdrawal or avoidance is safe in high-risk kidney transplants: a systematic review and meta-analysis. Kaohsiung J Med Sci. 2019;35(6):350-7. doi: 10.1002/kjm2.12064, PMID 30942560.

Storbeck KH, Schiffer L, Baranowski ES, Chortis V, Prete A, Barnard L. Steroid metabolome analysis in disorders of adrenal steroid biosynthesis and metabolism. Endocr Rev. 2019;40(6):1605-25. doi: 10.1210/er.2018-00262, PMID 31294783.

Behnaz Hassanbaglou. Antioxidant activity of different extracts from leaves of Pereskia bleo (Cactaceae). J Med Plants Res. 2012;6(15):2932-37. doi: 10.5897/JMPR11.760.

Kopustinskiene DM, Bernatoniene J, Jakstas V, Morkuniene R. Catechins, neuroprotection, and brain mitochondria. In: Mitochondrial physiology and vegetal molecules. Amsterdam, the Netherlands: Elsevier; 2021. p. 455-70.

Tapas A, Sakarkar DM, Kakde RB. Flavonoids as nutraceuticals: a review. Trop J Pharm Res. 2008;7:1089-99.

Amic D, Davidovic Amic D, Beslo D, Trinajsti N. Structure-radical scavenging activity relationships of flavonoids. Croat Chem Acta. 2003;76:55-61.

Christian GD, Dasgupta PK, Schug KA. Analytical chemistry. 7th ed. New York: John Wiley & Sons; 2014. p. 368-412.

Silva Beltran NP, Ruiz Cruz S, Cira Chavez LA, Estrada Alvarado MI, Ornelas Paz JdJ, Lopez Mata MA. Total phenolic, flavonoid, tomatine, and tomatidine contents and antioxidant and antimicrobial activities of extracts of tomato plant. Int J Anal Chem. 2015;2015:1-10. doi: 10.1155/2015/284071.

Validation of analytical procedures: text and methodology. In: Guideline ICH harmonized tripartite. International Conference on Harmonisation of Technical Requirements for registration of pharmaceuticals for human use; 2022. p. R1.

Published

26-11-2022

How to Cite

SAPTARINI, N. M., MUSTARICHIE, R., HERAWATI, I. E., & HADISOEBROTO, G. (2022). ISOLATION, IDENTIFICATION, AND QUANTIFICATION OF MAJOR FLAVONOID IN LEAVES OF PERESKIA BLEO (KUNTH) DC. International Journal of Applied Pharmaceutics, 14(4), 106–110. https://doi.org/10.22159/ijap.2022.v14s4.PP19

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 > >>