PRELIMINARY PHYTOCHEMICAL SCREENING AND TO EVALUATE THE ANTHELMINTIC ACTIVITY OF HYDRO-ALCOHOLIC AND PETROLEUM ETHER EXTRACT OF NYCTANTHES ARBOUR-TRISTIS ROOT. (FAMILY-NYCTAGINACEAE)
Keywords:Albendazole, Earthworms (pheretima posthuma), Nycthanthes arbor tristis, Anthelmintic activity
Objective: To estimate the anthelmintic activity of hydro-alcoholic (methanol) and petroleum ether extract of Nyctanthes arbour-tristis (family-Nyctaginaceae) in conjugation with phytochemical screening.
Methods: The hydro-alcoholic and petroleum ether extract of the whole root part of the plant Nyctanthes arbour-tristis (family-Nyctaginaceae) was prepared. And studies Phytochemical constitutes by the various standard method. The anthelmintic activity of the plant was performed by a different extract of plant material were tested against adult Indian earthworms pheretima posthuma as test worms, the bioassay determines the time of paralysis and time of death. Albendazole is used as a standard reference drug.
Results: The present study shows the phytochemical analysis, the anthelmintic activity of the hydro-alcoholic and petroleum ether extract of the root of Nyctanthes arbour-tristis. Various phytochemical analyses revealed the presence of alkaloids, carbohydrates, flavonoids, tannin, phenol,, glycosides, saponins respectively. The anthelmintic activity of the plant extract showed significant results against all earthwormsConclusion: The study has shown that petroleum ether and methanolic extract of Nyctanthes arbortristis root have significantly determined anthelmintic activity. But the methanolic extract of Nyctanthes arbortristis root shown most significant anthelmintic activity as compared to petroleum ether extract.
Hamburger M, Hostettmann K. Biochemistry in plants: the link between phytochemistry and medicine. Phytochemicals 1991;30:3864-7.
Singh BR, Singh V, Ebibeni N, Singh RK. Antimicrobial and herbal drug resistance in enteric bacteria isolated from faecal droppings of common house lizard/gecko (Hemidactylus frenatus). Int J Microbiol 2013;13:340-848.
Perry BD, Randolph TF. In: Veterinary, Parasetology: International livestock, Research institute, Nairobi, Kenya; 1999. p. 84, 145.
Goodman and Gilmanâ€™s, The Pharmacological Basis of Therapeutics, Tenth Edition, International edition; 2001. p. 1121-37.
Kurian S, Josekumar VS. Phytochemical screening, antimicrobial activity and brine shrimp lethality bioassay of different extracts of Alysicarpus vaginalis var. nummularifolius (DC.) MIQ. (family: fabaceae). Int J Pharm Sci 2017;9:1-6.
Dilla Jose, S Pandiammal. Journal of Academia and Industrial Research; 2016. p. 2278-5213.
Kokate CK. Pharmacognosy. Nirali Publication; 2007. p. 97-132.
Woisky R, Salatino. Screening of Indian plants for biological activity. Part II. A. J Agric Res 1998;37:99-105.