UV VISIBLE SPECTROPHOTOMETRIC METHOD DEVELOPMENT AND VALIDATION OF DASATINIB IN BULK AND SOLID DOSAGE FORM

SAILI MADUR; VINOD MATOLE; MALLINATH KALSHETTI

doi:10.22159/ijcpr.2020v12i4.39089

Authors

SAILI MADUR D. S. T. S Mandal’s College of Pharmacy, Solapur 413004, Maharashtra, India
VINOD MATOLE D. S. T. S Mandal’s College of Pharmacy, Solapur 413004, Maharashtra, India
MALLINATH KALSHETTI D. S. T. S Mandal’s College of Pharmacy, Solapur 413004, Maharashtra, India

DOI:

https://doi.org/10.22159/ijcpr.2020v12i4.39089

Keywords:

Dasatinib, UV visible spectrophotometer, Methanol, Method development and validation

Abstract

Objective: A new, simple, sensitive, precise, reproducible UV visible spectrophotometric method was developed for the determination of Dasatinib in Tablet dosage form with methanol.

Methods: The method is based on the formation of a colorlesscomplex. The UV spectrum of Dasatinib in methanol showed maximum wavelength at 248 nm. Beer’s law is valid in the concentration range of 7-35µg/ml. this method was validated for linearity, accuracy, precision, assay, ruggedness and robustness.

Results: The method has demonstrated excellent linearity over the range of 7-35µg/ml with the regression equation y = 0.0332x+0.0633 and regression coefficient i.e. r²= 0.9994moreover, the method was found to be highly sensitive with LOD(1.08µg/ml) and LOQ(3.29µg/ml).

Conclusion: Based on the results the proposed method can be successfully applied for the assay of Dasatinib in various tablet dosage forms.

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