• Muhammad Dawood Shah Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota-Kinabalu, Sabah, Malaysia
  • Yoong Soon Yong Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota-Kinabalu, Sabah, Malaysia
  • Mohammad Iqbal Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota-Kinabalu, Sabah, Malaysia


Objective: In the current study, the essential oil, methanol extract and methanol fractions (n-hexane, chloroform, ethyl acetate and n-butanol) of Nephrolepis biserrata L. were evaluated.

Methods: Preliminary phytochemical screening was done. The antioxidative effect was determined using 2,2diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity. Total phenol and flavonoid contents were calculated using Folin-Ciocalteau and aluminium chloride reagents. The phytochemical analysis of the essential oil, methanol extract and methanol fractions were performed by gas chromatography - mass spectrometry (GCMS).

Results: The preliminary screening confirmed the presence of active chemical constituents such as anthraquinones, alkaloids, tannins, steroids, phytosterol, saponin, triterpinoids and flavonoids. Our results also indicated that essential oil, methanol extract and methanol fractions are rich in phenolic and flavonoid contents. The GCMS analysis of our samples showed the presence of various biological important compounds. The dominant compounds are benzeneacetaldehyde, alpha.-cubebene, butyrolactone, phenol, benzyl alcohol, phenol, 2-methoxy-, 4h-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl, 2h-pyran-2-one, 4,6-dimethyl-, catechol, benzofuran, 2,3-dihydro-, phenol, 2,4-bis(1,1-dimethylethyl), hexadecanoic acid, methyl ester, n-hexadecanoic acid, 9,12-octadecadienoic acid, methyl ester, phytol, gamolenic acid and octadecanoic acid.

Conclusion: Our results demonstrate that the essential oil, methanol extract and methanol fractions of the N. biserrata represent a good source of potential bioactive compounds that could be used in pharmaceutical industry.


Keywords: Nephrolepis biserrata, Methanol extract, Antioxidant activity, Total phenolic, Flavonoids, Phytochemicals, GCMS analysis.


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1. Bloknina O, Virolainen E, Fagerstedt KV. Antioxidant, oxidative damage and oxygen deprivation studies: a review. Ann Bot 2003;91:179-94.
2. Fridovich L. The biology of oxygen radicals. Sci 1978;20:875-80.
3. Kinsella JE, Frankel E, German B, Kanner J. Possible mechanisms for the protective role of antioxidants in wine and plant foods. Food Technol 1993;47:85-9.
4. Valko M, Rhodes CJ, Moncol J, Izakovic M, Mazur M. Free radicals, metals and antioxidants in oxidative stress-induced cancer. Chem Biol Interact 2006;160:1-40.
5. Willcox JK, Ash SL, Catignani GL. Antioxidants and prevention of chronic disease. Crit Rev Food Sci Nutr 2004;44:275-95.
6. Diaz MN, Frei B, Vita JE, Keaney JF. Antioxidants and atherosclerotic heart disease. N Eng J Med 1997;337:408-16.
7. Aruoma OI. Free radicals, oxidative stress and antioxidants in human health and disease. J Am Oil Chem Soc 1998;75:199-212.
8. Saura-Calixto F, Goni L. Antioxidant capacity of the Spanish mediterranean diet. Food Chem 2006;94:442-7.
9. Mansouri A, Embarek G, Kokkalou E, Kefalas P. Phenolic profile and antioxidant activity of the Algerian ripe date palm fruit (Phoenix dactylifera). Food Chem 2005;89:411-20.
10. Siddhuraju P, Klaus B. Antioxidant properties of various solvent extracts of total phenolic constituents from the different agroclimatic origins of drumstick tree (Moringa oleifera Lam. ) leaves. J Agric Food Chem 2003;51:2144-55.
11. Kulip J, Fan LN, Manshoor N, Julius A, Said IM, Gisil J, et al. Medicinal plants in Maliau Basin, Sabah, Malaysia. J Trop Biol Cons 2010;6:21-33.
12. Christensen H. Uses of Ferns in two indigenous communities in Sarawak, Malaysia. In: Johns RJ, editor. Holttum Memorial Volume United Kingdom: The Royal Botanic Gardens, Kew; 1997. p. 177-92.
13. Boonkerd T, Chantanaorrapint S, Khwaip W. Pteridophyte diversity in the tropical lowland rainforest of Khao Nan National Park, Nakhon Si Thammarat Province, Thailand. NHJCU 2008;8:83-97.
14. Langeland KA. Natural area weeds: distinguishing native and non-native "Boston Ferns" and "Sword Ferns" (Nephrolepis spp. ). Publication SSAGR22. University of Florida. 2001;3-6.
15. Yussuf M, Wong C. A short botanical survey of brunia plants and some preliminary results of antimicrobial screening. http: //www. aseanbiodiversity. info/Abstract/53000244. pdf. 2012. Visited 4th April 2012.
16. Piggott AG. Ferns of Malaysia in color. Kuala Lumpur: Tropical press; 1996.
17. Khan RA, Khan MR, Sahreen S, Shah, NA Bokhari J, Rashid U, et al. Phytotoxic characterization of various fractions of Launaea nudicaulis. J Med Plants Res 2012;6:1403-6.
18. Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products. J Agric Food Chem 1998;46:4113-7.
19. Zou YP, Lu YH, Wei DZ. Antioxidant activity of a flavonoid-rich extract of Hypericum perforatum L. invitro. J Agric Food Chem 2004;52:5032-9.
20. Hatano T, Kagawa H, Yasuhara T, Okuda T. Two new flavonoids and other constituents in licorice root: their relative astringency and radical scavenging effects. Chem Pharm Bull 1988;36:1090-2097.
21. Harborne JB. Phytochemical methods. A guide to modern techniques of plant analysis. 3rd ed. New York: Chapman and Hall Int; 1998.
22. Shakirin FH, Prasad KN, Ismail A, Yuon LC, Azlan A. Antioxidant capacity of underutilized Malaysian Canarium odontophyllum (dabai) Miq fruit. J Food Compos Anal 2010;23:777-81.
23. Dixon RA, Dey PM, Lamb CJ. Phytoalexins: enzymology and molecular biology. Adv Enzymol Relat Areas Mol Biol 1983;55:61-9.
24. Trueba GP, Sanchez GM. Los flavonóides como antioxidantes naturales. Acta Farm
25. Bonaerense 2001;20:297-306.
26. Corrêa MFP, Melo GO, Costa SS. Substâncias de origem vegetal potencialmente úteis na terapia da Asma. Rev Bras Farmacogn 2008;18:785-97.
27. Yu LL. Free radical scavenging properties of conjugated linoleic acids. J Agric Food Chem 2001;49:3452-6.
28. Huang DJ, Ou BX, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.
29. Prior RL, Wu X, Schaich K. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. J Agric Food Chem 2005;53:4290-302.
30. Hossain MA, Nagooru MR. Biochemical profiling and total flavonoids contents of leaves crude extract of endemic medicinal plant Corydyline terminalis L. Kunth. Phcog J 2011;3:25-9.
31. Suresh SN, Nagarajan N. Preliminary phytochemical and antimicrobial activity analysis of Begonia malabarica Lam. J Basic Appl Bio 2009;3:59-61.
32. Dave H, Ledwani L. Review on anthraquinones isolated from Cassia species and their application. IJNPR 2012;3:291-319.
33. Cordell GA. Introduction to Alkaloids. A biogenic approach. New York: Wiley; 1983.
34. Heslem E. Plant Polyphone: vegetable tannin relisted chemistry and pharmacology of natural products, Cambridge: University press; 1989. p. 16.
35. Geuns JMC. Steroid hormones and plant growth and development. Phytochem 1978;17:I-14.
36. Aina DO, Wada CH. Preliminary phytochemical screening of Nimoalasis. WJLSMR 2005;2:182.
37. Sodipo OA, Akiniyi JA, Ogunbamosu JU. Studies on certain characteristics of extracts of bark of pansinystalia macruceras (Kschemp) pierre Exbeille. GJPAM 2000;6:83-7.
38. Moreau RA, Whitaker BD, Hicks KB. Phytosterols, phytosterol, and their conjugates in foods: Structural diversity, quantitative analysis, and health-promoting uses. Prog Lipid Res 2002;41(6):457-500.
39. Ovensná Z, Vachalková A, Horváthová K, Táthová D. Pentacyclic triterpenoic acids: new chemoprotective compounds. Neoplasma 2004;51:327-33.
40. Abadi A, Hassani A. Chemical composition and antibacterial activity of essential oil of Marrubium vulgare from eastern Algeria. ILCPA 2013;10(1):61-9.
41. Kochi M, Takeuchi S, Mizutani T, Mochizuki K, Matsumoto Y, Saito Y. Antitumor activity of benzaldehyde. Cancer Treat Rep 1980;64(1):21-3.
42. Behzad S, Pirani A, Mosaddegh M. Cytotoxic activity of some medicinal plants from Hamedan district of Iran. IJPR 2014;13:199-205.
43. Liu XQ, Gao YW, Guo YQ, Zhang TJ, Yan L. Two new a, b-unsaturated butyrolactone derivatives from Pleione bulbocodioides. Chin Chem Lett 2007;18:1075-77.
44. Dewi RT, Darmawan A, Tachibana S. Effect on a-glucosidase inhibition and antioxidant activities of butyrolactone derivatives from Aspergillus terreus MC751. Med Chem Res 2014;23:454-60.
45. Salat K, Librowski T, Moniczewski A, Stanisz-Wallis K, Wieckowski K, Malawska B. Analgesic, antioedema tous and antioxidant activity of γ-butyro lactone derivatives in rodents. Behav Pharmacol 2012;23(4):407-16.
46. Saravanan P, Chandramohan G, Mariajancyrani J, Shanmugasundaram P. GC-MS analysis of phytochemical constituents in ethanolic bark extract of Ficus Religiosa Linn. Int J Pharm Pharm Sci 2014;6(1).
47. Rao K, Ch B, Narasu LM, Giri A. Antibacterial activity of Alpinia galanga (L) Willd crude extracts. Appl Biochem Biotechnol 2010;162(3):871-84.
48. Lee KG, Shibamoto T. Determination of antioxidant potential of volatile extracts isolated from various herbs and spices. J Agric Food Chem 2002;50:4947-52.
49. Lee SJ, Umano K, Shibamoto T, Lee KG. Identification of volatile components in basil (Ocimum basilicum L. ) and thyme leaves (Thymus vulgaris L. ) and their antioxidant properties. Food Chem 2005;91:131-7.
50. Dasgupta A, Humphrey PE. Gas chromatographic-mass spectrometric identification and quantitation of benzyl alcohol in serum after derivatization with perfluorooctanoyl chloride: A new derivative. J Chromatogr B Biomed Sci App 1998;708(1-2):299-303.
51. Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. AJBR 2010;4(7):191-5.
52. Elezabeth VD, Arumugam S. GC-MS Analysis of ethanol extract of Cyperus rotundus leaves. Int J Curr Biotechnol 2014;2(1):19-23.
53. Appendino G, Ottino M, Marquez N, Bianchi F, Giana A. Ballero M, et al. Arzanol, an anti-inflammatory and anti-HIV-1 phloroglucinol α-pyrone from Helichrysum italicum ssp, microphyllum. J Nat Prod 2007;70(4):608-12.
54. Zheng LT, Ryu GM, Kwon B, Lee WH, Suk K. Anti-inflammatory effects of catechols in lipopolysaccharide-stimulated microglia cells: inhibition of microglial neurotoxicity. Eur J Pharmacol 2008;588:106-13.
55. Ramalakshmi S, Muthuchelian K. Analysis of bioactive constituents from the ethanolic leaf extract of Tabebuia rosea (Bertol. ) DC by gas chromatography-mass spectrometry. Int J Chem Tech Res 2011;3(3):1054-9.
56. Ajayi GO, Olagunju JA, Ademuyiwa O, Martins OC. Gas chromatography-mass spectrometry analysis and phytochemical screening of ethanolic root extract of Plumbago zeylanica, Linn. J Med Plants Res 2011;5(9):1756-61.
57. Anand T, Gokulakrishnan K. Phytochemical analysis of Hybanthus enneaspermus using UV, FTIR and GC-MS. IOSRJP 2012;2(3):520-4.
58. Kapoor R, Huang YS. Gamma Linolenic Acid: an antiinflammatory omega-6 Fatty acid. Curr Pharm Biotechnol 2006;7(6):531-4.
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How to Cite
Shah, M. D., Y. S. Yong, and M. Iqbal. “PHYTOCHEMICAL INVESTIGATION AND FREE RADICAL SCAVENGING ACTIVITIES OF ESSENTIAL OIL, METHANOL EXTRACT AND METHANOL FRACTIONS OF NEPHROLEPIS BISERRATA”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 6, no. 9, Sept. 2014, pp. 269-77,
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