ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF LYGODIUM MICROPHYLLUM IN ALLOXAN INDUCED DIABETIC RATS

  • D. G. Syahidah Nadiah Binti Abdull Majid Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MALAYSIA
  • Mohammad Iqbal Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, MALAYSIA

Abstract

Objective: The antihyperglycemic and antioxidative effects of L. microphyllum were evaluated by using in vivo methods in normal and alloxan induced diabetic rats.


Methods: Diabetes was induced in Sprague Dawley rats by injecting alloxan through intravenous (i. v) at a dose of 100 mg/kg of body weight. Aqueous extract of L. microphyllum at different doses (400, 200 and 100 mg/kg of body weight) was administered orally (orogastric intubation) for 14 d. Blood glucose and oxidative stress markers were measured. Hematoxylin and eosin staining method were used to examine the pancreatic tissues.


Results: At the 14 d interval, fasting blood glucose showed a reduction in serum glucose levels in animals pretreated with L. microphyllum compared with alloxan alone treated group. Oxidative stress was noticed in rat’s pancreatic tissue as evidenced by a significant decrease in glutathione level, glutathione reductase, glutathione-S-transferase, and catalase activities. Malondialdehyde showed a significant increase compared to the normal saline-treated control group. Serum biochemistry and oxidative stress markers were consistent with the pancreatic histopathological studies. Treatment of diabetic rats with L. microphyllum at a dose level of 100, 200 and 400 mg/kg body weight leaves extract for 14 d significantly prevented these alterations and attenuated alloxan-induced oxidative stress (P<0.05).


Conclusion: The results of the present study indicated that the antihyperglycemic potential of L. microphyllum might be ascribable to its antioxidant and free radical scavenging properties. Thus, it is concluded that L. microphyllum may be helpful in the prevention of diabetic complications associated with oxidative stress.

Keywords: L. microphyllum, Antihyperglycemic, Antioxidative, Alloxan, Diabetes mellitus

Downloads

Download data is not yet available.

References

1. Kalaiselvan M, Gopalan RT. Ethnobotanical studies on selected wild medicinal plants used by Irula tribes of bola patty valley, nilgiri biosphere reserve (NBR), and Southern Western ghats, India. Asian J Pharm Clin Res 2014;7:22-6.
2. Nazeema BB, Julie J, Abirami J, Kumaresan R, Muthukumaran T, Rajasree S, et al. Anti-cancer activity of Datura metel on MCF-7 cell line. Asian J Pharm Clin Res 2014;7:181-3.
3. Chen CY, Chiu FY, Lin Y, Huang WJ, Hsieh PS, Hsu FL. Chemical constituent analysis and antidiabetic activity validation of four fern species from taiwan. Int J Mol Sci 2015;16:2497-516.
4. Zhang XF, Tan BK. Effects of an ethanolic extract of Gynura procumbens on serum glucose, cholesterol and triglyceride levels in normal and streptozotocin-induced diabetic rats. Singapore Med J 2000;41:9-13.
5. Ashraduzzaman MD, Shahanaz K, Shabnam B, Nurul A. Vigna unguiculata Linn. Walp. Seed oil exhibiting antidiabetic effects in alloxan-induced diabetic rats. Malay J Pharm Sci 2011;9:13-23.
6. Hussein Z, Taher SW, Gilchandran Singh HK, Swee WC. Diabetes care in malaysia: problems, new models, and solutions. Ann Glob Health 2015;81:851-62.
7. Gunggu A, Thon CC, Lian CW. Predictors of diabetes self-management among type 2 diabetes patients. J Diabetes Res 2016;1-7. Doi:10.1155/2016/9158943
8. Mafauzy M. Diabetes mellitus in Malaysia. Med J Malaysia 2006;61:397-8.
9. Mir MS, Darzi MM, Musadiq KH, Kamil SA, Sofi AH, Wani SA. Pathomorphological effects of alloxan-induced acute hypoglycemia in rabbits. Alex J Med 2013;49:343-53.
10. Cyriac B, Eswaran K. Anti-hyperglycemic effect of aqueous extract of Kappaphycus alvarezii (Doty) doty ex. P. Silva in alloxan-induced diabetic rats. J Appl Phycol 2015;28:1-7.
11. Azwanida NN. A review on the extraction methods uses in medicinal plants, principle, strength, and limitation. Med Aromat Plants J 2015;4:196-202.
12. Jollow DJ, Mitchell JR, Zampaglione N, Gillette JR. Bromobenzene-induced liver necrosis: protective role of glutathione and evidence for 3,4-bromobenzeneo hepatotoxic intermediate. Pharm 1974;11:151-69.
13. Buege JA, Aust SD. Microsomal lipid peroxidation. Method Enzyme 1978;52:302-10.
14. Carlberg I, Mannervik B. Purification and characterization of the flavoenzyme glutathione reductase from rat liver. J Bio Chem 1975;250:5475-80.
15. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferase: the first enzymatic step in mercapturic acid formation. J Bio Chem 1974;249:7130-9.
16. Claiborne A. Catalase activity. In: Green Wald RA. (ed.) CRC handbook of methods for oxygen radical research. CRC Press: Boca Raton; 1985. p. 283–4.
17. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Bio Chem 1951;193:265-75.
18. Sangeetha MS, Priyanga S, Hemmalakshmi S, Devaki K. In vivo antidiabetic potential of Cyclea peltate in streptozotocin-induced diabetic rats. Asian J Pharm Clin Res 2015;8:103-8.
19. Mohan Y, Jesuthankaraj GN, Thangavelu NR. Antidiabetic and antioxidant properties of Triticum aestivum in streptozotocin-induced diabetic rats. Adv Pharmacol Sci 2013;1-9. http:// dx.doi.org/10.1155/2013/716073.
20. Sunday JJ, Spencer NCO, Kingsley O, Akintola AA, Binyelum N, Favour AO. Possible revival of atrophied islets cells of the pancreas by Vernonia amygdalina in alloxan induced diabetic rats. J Appl Pharm Sci 2012;2:127-31.
21. Nugent DA, Smoth DM, Jones HW. A review of islets of langerhans degeneration in rodent models of type 2 diabetes. Toxic Path 2008;36:529-51.
Statistics
103 Views | 233 Downloads
How to Cite
Abdull Majid, D. G. S. N. B., and M. Iqbal. “ANTIHYPERGLYCEMIC AND ANTIOXIDATIVE EFFECTS OF LYGODIUM MICROPHYLLUM IN ALLOXAN INDUCED DIABETIC RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 10, no. 12, Dec. 2018, pp. 75-79, doi:10.22159/ijpps.2018v10i12.29232.
Section
Original Article(s)