IN VITRO INHIBITORY ACTIVITY OF MEDICINAL PLANTS AGAINST PHOMOPSIS AZADIRACHTAE, THE INCITANT DIE BACK DISEASE OF NEEM
Keywords:Neem, Phomopsis azadirachtae, Medicinal plants, Antifungal activity, Plant extract
Objectives: Plant diseases extend a threat to the global production of herbal medicines. Safer plant protection strategies are in need to meet the demands from the pharmaceutical industry. Neem is a tree with great medicinal values, which is being infected by Phomopsis azadirachtae (PA), causing die back disease. The resulting massive destruction of the tree demands optimum disease management methods and thus, the objective of the present study was to evaluate the inhibitory potential of some plant extracts against the growth of PA.
Methods: In this study, we investigate the in vitro antifungal activity of ether: chloroform (1:1), ethyl acetate, acetone and ethanol extracts from different parts (leaves and roots) of five plant species viz., Ocimum tenuiflorium, Centella asiatica, Solanum nigrum, Apium graveolens and Besella alba against the growth of PA.
Results: All the 40 crude extracts (at concentration of 1000 Î¼g/ml), were active against PA. Among them, the ethanol and ethyl acetate extracts proved optimum with antifungal activity ranging from 65-95% and minimum inhibitory concentration (MIC) values from 75-200 Î¼g/ml. The antifungal activity of the extracts was in the order: ethanol>ethyl acetate>acetone>ether: chloroform (1:1). Also, noteworthy results were observed in all the extracts tested against the spore germination of PA, with concentration and time-dependent kinetics.
Conclusion: The results prove promising in order to develop new antifungal agents against the growth and spread of PA.
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