IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES


Ramith Ramu, Prithvi S. Shirahatti, Shrisha Naik Bajpe, Varsha Reddy S V, M. N. Nagendra Prasad

Abstract


Objective: The present study was designed to evaluate for cytoprotective and DNA protective properties of the compounds isolated from ethanol extract of banana flower (EF) and ethanol extract of banana pseudostem (EE).

Methods: The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and β-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals.

Results: The study revealed that the compounds (C1-C4) at a concentration of 1 mg/ml exhibited 90% protection on erythrocytes membrane oxidation and also protect the pTZ57R/T plasmid DNA damage induced by hydroxyl radicals.

Conclusion: These results endorse an insight for a strong chemical basis to the alleged beneficial role of EF and EE in reducing oxidative stress conditions.

 


Keywords


Umbelliferone, Lupeol, Stigmasterol, β-Sitosterol

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About this article

Title

IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES

Keywords

Umbelliferone, Lupeol, Stigmasterol, β-Sitosterol

DOI

10.22159/ijpps.2017v9i10.20560

Date

02-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol. 9, Issue 10, 2017 Page: 21-23

Online ISSN

0975-1491

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Authors & Affiliations

Ramith Ramu
Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Ujire, Dakshina Kannada – 574240, Karnataka, India
India

Prithvi S. Shirahatti
Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Ujire, Dakshina Kannada – 574240, Karnataka, India
India

Shrisha Naik Bajpe
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysore 570006, Karnataka, India
India

Varsha Reddy S V
Department of Biotechnology, Sri Dharmasthala Manjunatheshwara College of Post Graduate Centre, Ujire, Dakshina Kannada – 574240, Karnataka, India
India

M. N. Nagendra Prasad
Department of Biotechnology, Sri Jayachamarajendra College of Engineering, JSS Institution Camp, Manasagangothri, Mysore – 570 006, Karnataka, India


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