IMPACT OF ACTIVE COMPOUNDS ISOLATED FROM BANANA (MUSA SP. VAR. NANJANGUD RASABALE) FLOWER AND PSEUDOSTEM TOWARDS CYTOPROTECTIVE AND DNA PROTECTION ACTIVITIES
Objective: The present study was designed to evaluate for cytoprotective and DNA protective properties of the compounds isolated from ethanol extract of banana flower (EF) and ethanol extract of banana pseudostem (EE).
Methods: The four active compounds viz., umbelliferone (C1) andlupeol (C2) from EF and stigmasterol (C3) and Î²-sitosterol (C4) from EE were isolated by activity-guided repeated fractionation through silica gel column chromatography. The isolated compounds were evaluated for cytoprotective on erythrocytes and pTZ57R/T plasmid DNA protection against hydroxyl radicals.
Results: The study revealed that the compounds (C1-C4) at a concentration of 1 mg/ml exhibited 90% protection on erythrocytes membrane oxidation and also protect the pTZ57R/T plasmid DNA damage induced by hydroxyl radicals.
Conclusion: These results endorse an insight for a strong chemical basis to the alleged beneï¬cial role of EF and EE in reducing oxidative stress conditions.
2. Someya S, Yoshiki Y, Okubo K. Antioxidant compounds from bananas (Musa cavendish). Food Chem 2002;79:351â€“4.
3. Kanazawa K, Sakakibara H. High content of dopamine, a strong antioxidant in Cavendish banana. J Agric Food Chem 2000;48:844â€“8.
4. Pari L, Umamaheshwari J. Antihyperglycemic activity of Musa sapientum flowers-effect on lipid peroxidation in alloxan diabetic rats. Phytother Res 2000;14:136-8.
5. Hai Q, Wen LH, Xiao MW, Hui BZ, Jin PZ, Wen CY. A new isochroman-4-one derivative from the peel of Musasapientum (L) and its total synthesis. Chinese Chem Lett 2007;18:1227-30.
6. Waalkes TP, Sjoerdsma A, Creveling CR, Weissbach H, Udenfriend S. Serotonin, norepinephrine and related compounds in bananas. Science 1958;127:648-53.
7. Pellai N, Aashan TN. Ayurveda Prakashika. (Reddiar and Son). Vidyarambha Press: Quilon, India; 1955. p. 97âˆ’115.
8. Goel RK, Sairam K. Anti-ulcer drugs from indigenous sources with emphasis on Musa sapientum, Tamrabhasma, Asparagus racemosus and Zingiber officinale. Indian J Pharmacol 2002;34:100-10.
9. Lewis DA, Fields WN, Shaw GP. A natural flavonoid present in unripe plantain banana pulp (Musasapientum L. var. paradisiaca) protects the gastric mucosa from aspirin-induced erosions. J Ethnopharmacol 1999;65:283-8.
10. Gomathy R, Vijayalakshmi NR, Kurup PA. Hypolipidemic principle of the inflorescence stalk of plantain (Musa sapientum). J Bioscien 1989;14:301-9.
11. Anonymous. The Wealth of India. Council of Scientific and Industrial Research: New Delhi, India; 2003. p. 178â€“85.
12. Jain DL, Baheti AM, Ingale SP, Ingale PL, Parakh SR. Study of antacid and diuretic activity of ash and extracts of Musa sapientum (L) fruit peel. Pharmacogn Magaz 2007;3:116-9.
13. Devasagayam TPA, Tilak JC, Boloor KK, Ketaki SS, Saroj SG, Lele RD. Free radicals and antioxidants in human health: Current status and future prospects. J Assoc Phys India 2004;52:794â€“804.
14. Jamuna JB, Mahadevamma S, Nandini D, Salimath PV. Banana (Musa sp. var. elakki bale) flower and pseudostem: dietary fiber and associated antioxidant capacity. J Agric Food Chem 2012;60:427-32.
15. Ramith R, Prithvi SS, Farhan Z, Lakshmi VR, Nagendra MNP. Inhibitory effect of banana (Musa sp. var. Nanjangud rasa bale) flower extract and its constituents Umbelliferone and Lupeol on Î±-glucosidase, aldose reductase and glycation at multiple stages. South Afr J Bot 2014;95:54â€“63.
16. Ramith R, Prithvi SS, Nanjunda SS, Zameer F, Bhadrapura LD, Nagendra PMN. Assessment of in vivo antidiabetic properties of umbelliferone and lupeol constituents of banana (Musa sp. var. Nanjangud Rasa Bale) flower in hyperglycaemic rodent model. PLoS One 2016;11:e0151135. Doi:10.1371/journal.pone.0151135.
17. Lee JC, Kim J, Jang YS. Antioxidant property of an ethanol extract of the stem of opuntia ficus-indica var. saboten. J Agric Food Chem 2002;5:6490-6.
18. Suwalsky M, Orellana P, Avello M, Villena F. Protective effect of Ugni molinae Turcz against oxidative damage of human erythrocytes. Food Chem Toxicol 2007;45:130â€“5.
19. Harish NMA, Sathisha UV, Shylaja MD. Cytoprotective and antioxidant activity of free, conjugated and insoluble-bound phenolic acids from swallow root (Decalepis hamiltonii). Food Chem 2010;119:1307â€“12.
20. Harish NMA, Sathisha UV, Manohar MP, Chandrashekar KB, Shylaja MD. Cytoprotective and antioxidant activity studies of jaggery sugar. Food Chem 2009;115:113â€“8.
21. Hazrulrizawati AH, Senait SZ, Mashitah MY. Rhodomyrtustomentosa: a phytochemical and pharmacological review. Asian J Pharm Clin Res 2017;10:10â€“6.