IDENTIFICATION OF PROTEINS ISOLATE FROM AMARANTH (AMARANTHUS CAUDATUS) BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS WITH WATER AND NACL 0.1 M SOLVENTS

  • Wilman Carrillo Universidad Técnica de Ambato http://orcid.org/0000-0002-7609-2939
  • Crsitina Acosta Universidad Técnica de Ambato
  • Rubén Vilcacundo Universidad Técnica de Ambato
  • Cecilia Carpio Universidad Técnica de Ambato

Abstract

ABSTRACT
Objective: The aim of this study was to obtain protein isolate from amaranth using alkaline method at extraction pH 8 and extraction pH 12 with
different precipitation pHs and to analyze protein isolate with electrophoresis.
Methods: Amaranth protein isolates were obtained using isoelectric precipitation method at different pHs. Proteins were analyzed using sodium
dodecyl sulfate-polyacrylamide gel electrophoresis.
Results: A yield of 20.52% of protein isolate of defatted amaranth at pH 4.0 was obtained. The content of protein isolate was higher than 53% in all
pH assays. Globulins and albumins in protein isolate at different pHs were observed. A band with 50 kDa corresponding to 7S globulin was found. The
bands 36-38 kDa and 18-20 kDa correspond to 11S globulin. Bands less to 14.4 kDa correspond to albumins.
Conclusions: Amaranth protein isolate is possible to obtain in extreme conditions of pH. The treatment with water was optimum to obtain amaranth
protein isolate.
Keywords: Amaranth, Globulins, Albumins, Proteinisolate, Sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

Author Biographies

Wilman Carrillo, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeería en Aliementos
Crsitina Acosta, Universidad Técnica de Ambato
Facultad de Ciencia e Ingeería en Aliementos
Rubén Vilcacundo, Universidad Técnica de Ambato
Facultad de Ciencia e Ingenería en Alimentos
Cecilia Carpio, Universidad Técnica de Ambato
Facultad de Ciencia e Ingenería en Alimentos

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How to Cite
Carrillo, W., C. Acosta, R. Vilcacundo, and C. Carpio. “IDENTIFICATION OF PROTEINS ISOLATE FROM AMARANTH (AMARANTHUS CAUDATUS) BY SODIUM DODECYL SULFATE-POLYACRYLAMIDE GEL ELECTROPHORESIS WITH WATER AND NACL 0.1 M SOLVENTS”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 3, May 2016, pp. 331-4, https://innovareacademics.in/journals/index.php/ajpcr/article/view/11446.
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