• Urip Harahap
  • Dadang Irfan Husori Department of Pharmacology, Faculty of Pharmacy, University of Sumatera Utara, Medan, Indonesia
  • Marianne
  • Sri Yuliasmi
  • Popi Patilaya
  • Lia Laila
  • Bayu Eko Prasetyo
  • Henny Sri Wahyuni
  • Imam Bagus Sumantri



Objective: The study was aimed to investigate the inhibitory effect of ethanolic extract of Curanga fel-terrae leaves (EECFL) against acetylcholine

(ACh)-induced contraction of the ACh-muscarinic-3 receptor.

Materials and Methods: The study of the inhibitory effect of the ethanolic extract on the contraction by ACh concentration series (10−8-10−3  M) was conducted in vitro using isolated guinea pig tracheal organ in the Krebs solution.

Results: Early incubation of tracheal organ with EECFL (0.5, 1, 2 and 4 mg/ml) before contracted by the series of ACh concentration produces the decrease of ACh contraction. The concentration series of ACh sigmoid curve rightward shift without decreasing the maximal contraction. The results of double-reciprocal plot of ACh shows the mean value of the 1/y-intercept of each extract was not different with the control.

Conclusion: The EECFL showed competitive antagonist effect on ACh-muscarinic-3 receptors.

Keywords: Curanga fel-terrae, Pugun tano, Acetylcholine-muscarinic-3 receptors, Competitive antagonist, Ethanolic extract.


Download data is not yet available.


van Valkenburg JL, Bunyapraphatsara N, editors. Plant Resources of South-East Asia No. 12 (2): Medicinal and Poisonous Plants 2. Leiden: Backhuys Publishers; 2001.

glycosides from Picria fel-terrae Lour. J Integr Plant Biol 2005;47(5):632-6.

Huang Y, De Bruyne T, Apers S, Ma Y, Claeys M, Vanden Berghe D, et al. Complement-inhibiting cucurbitacin glycosides from Picria fel- terrae. J Nat Prod 1998;61(6):757-61.

Huang Y, De Bruyne T, Apers S, Ma Y, Claeys M, Pieters L, et al. Flavonoid glucuronides from Picria fel-terrae. Phytochemistry 1999;62(8):1701-3.

Fang H, Ning DS, Liang XY. Studies on technology optimization for extracting triterpenoid saponins from Picria fel-terrae by multi-target grading method. J Chin Med Mater 2009;32(12):1902-5.

Wang LS, Li SH, Zou JM, Guo YJ, Sun HD. Two new triterpenoids from Picria fel-terrae. J Asian Nat Prod Res 2006;8(6):491-4.

Patilaya P, Husori DI. Preliminary study on the anthelmintic activity of the leaf ethanolic extract of Indonesian Curanga fel-terrae (Lour.) Merr. Int J Pharm Tech Res 2015;8(3):347-51.

Sitorus P, Harahap U, Pandapotan M, Barus T. Isolation of β-sitosterol from n-hexane extract of Picria fel-terrae Lour. Leave and study of its antidiabetic effect in alloxan induced diabetic mice. Int J PharmTech Res 2014;6(1):137-41.

Harfina F, Bahri S, Saragih A. Pengaruh serbuk Daun puguntano (Curanga fel-terrae Merr.) Pada pasien diabetes mellitus. J Pharm Pharmacol 2012;2(1):112-8.

Lestari P, Hadisahputra S, Ilyas S, Satria D. Combinational effects of n-hexane extract of poguntano leaves (Picria fel-terrae Lour.) With doxorubicin on MCF-7 breast cancer cells. J Chem Pharm Res 2015;7(5):353-5.

Satria D, Furqan M, Hadisahputra S, Rosidah. Combinational effects of ethylacetate extract of Picria fel-terrae Lour and doxorubicin on T47D breast cancer cells. Int J Pharm Pharm Sci 2015;7(7):73-6.

Furqan M, Hadisahputra S, Rosidah. Effects of inhibition cell cycle and apoptosis of poguntano leaves ethylacetate extract (Picria fel-terrae Lour.) on breast cancer cells. Int J PharmTech Res 2014;6(3):1096-9.

Dalimunthe A, Harahap U, Rosidah, Nasution MP. Evaluation of diuretic activity of Picria fel-terrae Lour leaves extracts. Asian J Pharm Clin Res 2015;8(4):204-5.

Sihotang Y, Silalahi J, Hadisahputra S, Anjelisa P, Satria D. Cardioprotective effect of ethylacetate extract of poguntano (Picria fel- terrae Lour.) Against doxorubicin-induced cardiotoxicity in rats. Int J Pharm Clin Res 2016;8 Suppl 5:466-70.

WHO. Prevention of Allergy and Allergic Asthma. Geneva: WHO Document and Publication Service; 2003.

WHO. Asthma. Geneva: WHO Document and Publication Service; 2008.

Ministry of Health of Republic of Indonesia. Health Profile. Jakarta: Kemenkes; 1997.

Gosens R, Zaagsma J, Meurs H, Halayko AJ. Muscarinic receptor signaling in the pathophysiology of asthma and COPD. Respir Res 2006;7:73.

Ikeda T, Anisuzzaman AS, Yoshiki H, Sasaki M, Koshiji T, Uwada J, et al. Regional quantification of muscarinic acetylcholine receptors and ß-adrenoceptors in human airways. Br J Pharmacol 2012;166(6):1804-14.

Carr R 3rd, Koziol-White C, Zhang J, Lam H, An SS, Tall GG, et al. Interdicting Gq activation in airway disease by receptor-dependent and receptor-independent mechanisms. Mol Pharmacol 2016;89(1):94-104.

Husori DI, Riyanto S, Nugroho AE. Relaxation effect of Marmin on guinea pig tracheal smooth muscle via NO-independent mechanisms. Asian Pac J Trop Dis 2012;2:S154-8.

Nugroho AE, Anas Y, Arsito PN, Wibowo JT, Riyanto S, Sukari MA. Effects of marmin, a compound isolated from Aegle marmelos Correa, on contraction of the guinea pig-isolated trachea. Pak J Pharm Sci 2011;24(4):427-33.

Nugroho AE, Wibowo JT, Riyanto S. Marmin, a compound from Aegle marmelos Corr., Relaxes the ovalbumin induced contraction of trachea. Int J Pharm Pharm Sci 2012;4 Suppl 1:479-84.

Srivastava RC, Nagappa AN. Surface Activity in Drug Action. Missouri: Elsevier Science Ltd.; 2005.

Plant PJ, North ML, Ward A, Ward M, Khanna N, Correa J, et al. Hypertrophic airway smooth muscle mass correlates with increased airway responsiveness in a murine model of asthma. Am J Respir Cell Mol Biol 2012;46(4):532-40.

Semenov I, Herlihy JT, Brenner R. In vitro measurements of tracheal constriction using mice. J Vis Exp 2012;. pii:3703.

Scott GD, Fryer AD. Role of parasympathetic nerves and muscarinic receptors in allergy and asthma. Chem Immunol Allergy 2012;98:48-69.

Karakiulakis G, Roth M. Muscarinic receptors and their antagonists in COPD: Anti-inflammatory and antiremodeling effects. Mediators Inflamm 2012;2012:409580.

Govindan S, Taylor CW. P2Y receptor subtypes evoke different Ca2 signals in cultured aortic smooth muscle cells. Purinergic Signal 2012;8(4):763-77.

Roux E, Molimard M, Savineau JP, Marthan R. Muscarinic stimulation of airway smooth muscle cells. Gen Pharmacol 1998;31(3):349-56.

Eglen RM, Hegde SS, Watson N. Muscarinic receptor subtypes and smooth muscle function. Pharmacol Rev 1996;48(4):531-65.

Villetti G, Pastore F, Bergamaschi M, Bassani F, Bolzoni PT, Battipaglia L, et al. Bronchodilator activity of (3R)-3-[[[(3- fluorophenyl)[(3,4,5-trifluorophenyl)methyl]amino] carbonyl]oxy]-

-[2-oxo-2-(2-thienyl)ethyl]-1-azoniabicyclo[2.2.2]octane bromide (CHF5407), a potent, long-acting, and selective muscarinic M3 receptor antagonist. J Pharmacol Exp Ther 2010;335(3):622-35.

Dambros M, van Deutekom M, de Jongh R, van Koeveringe GA, De Mey JG, van Kerrebroeck P. The inhibitory effect of the flavonoid galangin on urinary bladder smooth muscle contractility is mediated in part by modulation of Ca2 release from intracellular stores. Planta Med


Innocenti G, Dall’Acqua S, Minesso P, Budriesi R, Micucci M, Chiarini A. Evaluation of muscarinic M3-receptor antagonism of Solenostemma argel leaves. Planta Med 2010;76:634.

Patil DS, Ahale SV, Surana SJ. Evaluation of antiasthmatic and antianaphylactic activity of Balanites aegyptiaca (Delile), (Balanitaceae). Asian J Pharm Clin Res 2011;4(1):52-5.

Speroni E, Cervellati R, Innocenti G, Costa S, Guerra MC, Dall’ Acqua S, et al. Anti-inflammatory, anti-nociceptive and antioxidant activities of Balanites aegyptiaca (L.) Delile. J Ethnopharmacol 2005;98(1-2):117-25.

Mahapatra PK, Pradhan D. Relaxant effects of Limonia acidissima Linn (pulp) on guinea pig tracheal chains and its possible mechanism(s). Int J Pharm Pharm Sci 2014;6(5):257-63.



How to Cite

Harahap, U., D. I. Husori, Marianne, S. Yuliasmi, P. Patilaya, L. Laila, B. E. Prasetyo, H. S. Wahyuni, and I. B. Sumantri. “INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF CURANGA FEL-TERRAE (PUGUN TANO) LEAVES ON ACETYLCHOLINE MUSCARINIC-3 RECEPTORS INDUCED ON ISOLATED GUINEA PIG TRACHEAL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 1, Jan. 2017, pp. 95-98, doi:10.22159/ajpcr.2017.v10i1.14163.



Original Article(s)

Most read articles by the same author(s)

1 2 3 4 > >>