INHIBITION OF CARSINOGENESIS BY SEED AND SOYBEAN MEAL EXTRACT IN COLON OF MICE: APOPTOSIS AND DYSPLASIA

Authors

  • Anindini Winda Amalia Faculty of Pharmacy, University of Indonesia
  • KUSMARDI Faculty of Medicine University of Indonesia
  • Berna Elya Faculty of Pharmacy University of Indonesia
  • ADE ARSIANTI Faculty of Medicine, University of Indonesia

DOI:

https://doi.org/10.22159/ajpcr.2017.v10i4.16276

Abstract

Objective: Colon cancer is a major public health problem. Soybean has demonstrated chemopreventive and anticancer. Here, we have investigated
the effect of a standardized seed and soybean meal extract (SE) with content of lunasin, here named grobogan extract (GE) and SE. They are botanical
drug substance in experimental models of colon cancer in vivo.

Methods: The effect of GE and SE was examined on the preneoplastic lesions (aberrant crypt foci), polyps and tumors induced by the carcinogenic
agent azoxymethane (10 mg/kg) and dextran sodium sulfate 2% as well as in a xenograft model of colon cancer in mice.

Results: GE and SE increased apoptosis (p=0.001). GE (150 mg/kg) has the highest impact level of apoptosis (p=0.009). GE and SE decreased dysplasia
(p=0.024). GE (200 mg/kg) has the highest impact level of dysplasia (p=0.002), and SE (200 mg/kg) has the second impact level of dysplasia (p=0.003).

Conclusions: GE and SE inhibition of colon carcinogenesis with increased level of apoptosis and decrease level of dysplasia.

Keywords:Soybean, Lunasin, Colon cancer, Azoxymethane, Dextran sodium sulfate, Apoptosis, Dysplasia.

Downloads

Download data is not yet available.

References

Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, Mathers C, et al. GLOBOCAN 2012 v1.1, Cancer Incidence and Mortality Worldwide: IARC Cancerbase No. 11. Lyon, France: International Agency for Research on Cancer; 2014. Available from: http://www.globocan.iarc. fr. [Last accessed on 2015 Jan 16].

Theodoratou E, Farrington SM, Tenesa A, McNeill G, Cetnarskyj R, Korakakis E, et al. Associations between dietary and lifestyle risk factors and colorectal cancer in the Scottish population. Eur J Cancer Prev 2014;23(1):8-17.

Kriza C, Emmert M, Wahlster P, Niederländer C, Kolominsky-Rabas P. Cost of illness in colorectal cancer: An international review. Pharmacoeconomics 2013;31(7):577-88.

Derry MM, Raina K, Agarwal C, Agarwal R. Identifying molecular targets of lifestyle modifications in colon cancer prevention. Front Oncol 2013;3:119.

Ebos JM, Kerbel RS. Antiangiogenic therapy: Impact on invasion, disease progression, and metastasis. Nat Rev Clin Oncol 2011;8(4):210-21.

Franceschi S, Wild CP. Meeting the global demands of epidemiologic transition - The indispensable role of cancer prevention. Mol Oncol 2013;7(1):1-13.

Coward L, Barnes N, Setchell K, Barnes S. Genistein, daidzein, and their beta-glycoside conjugates: Antitumor isoflavones in soybean food from American and Asian diets. J Agric Food Chem 1993;41(11):1961-7.

Wang H, Murphy P. Isoflavone content in commercial soybean foods. J Agric Food Chem 1994;42(8):1666-73.

Fournier DB, Erdman JW Jr, Gordon GB. Soy, its components, and cancer prevention: A review of the in vitro, animal, and human data. Cancer Epidemiol Biomarkers Prev 1998;7(11):1055-65.

Anderson JW, Smith BM, Washnock CS. Cardiovascular and renal benefits of dry bean and soybean intake. Am J Clin Nutr 1999;70 3 Suppl:464S-74.

Swanson CA, Mao BL, Li JY, Lubin JH, Yao SX, Wang JZ, et al. Dietary determinants of lung-cancer risk: Results from a case-control study in Yunnan Province, China. Int J Cancer 1992;50(6):876-80.

Wu AH, Ziegler RG, Horn-Ross PL, Nomura AM, West DW, Kolonel LN, et al. Tofu and risk of breast cancer in Asian-Americans. Cancer Epidemiol Biomarkers Prev 1996;5(11):901-6.

Goodman MT, Wilkens LR, Hankin JH, Lyu LC, Wu AH, Kolonel LN. Association of soy and fiber consumption with the risk of endometrial cancer. Am J Epidemiol 1997;146(4):294-306.

Jacobsen BK, Knutsen SF, Fraser GE. Does high soy milk intake reduce prostate cancer incidence? The Adventist Health Study (United States). Cancer Causes Control 1998;9(6):553-7.

Zheng W, Dai Q, Custer LJ, Shu XO, Wen WQ, Jin F, et al. Urinary excretion of isoflavonoids and the risk of breast cancer. Cancer Epidemiol Biomarkers Prev 1999;8(1):35-40.

Kolonel LN, Hankin JH, Whittemore AS, Wu AH, Gallagher RP, Wilkens LR, et al. Vegetables, fruits, legumes and prostate cancer: A multiethnic case-control study. Cancer Epidemiol Biomarkers Prev 2000;9(8):795-804.

Sun CL, Yuan JM, Arakawa K, Low SH, Lee HP, Yu MC. Dietary soy and increased risk of bladder cancer: The Singapore Chinese health study. Cancer Epidemiol Biomarkers Prev 2002;11(12):1674-7.

Messina M, Flickinger B. Hypothesized anticancer effects of soy: Evidence points to isoflavones as the primary anticarcinogens. Pharm Biol 2002;40(1):S6-23.

de Lumen BO. Lunasin: A cancer-preventive soy peptide. Nutr Rev 2005;63(1):16-21.

Lam Y, Galvez A, de Lumen BO. Lunasin suppresses E1A-mediated transformation of mammalian cells but does not inhibit growth of immortalized and established cancer cell lines. Nutr Cancer 2003;47(1):88-94.

Jeong HJ, Lam Y, de Lumen BO. Barley lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells. J Agric Food Chem 2002;50(1):5903-8.

Galvez AF, Chen N, Macasieb J, de Lumen BO. Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res 2001;61(20):7473-8.

Shogren-Knaak M, Ishii H, Sun JM, Pazin MJ, Davie JR, Peterson CL. Histone H4-K16 acetylation controls chromatin structure and protein interactions. Science 2006;311(5762):844-7.

Yasui W, Oue N, Ono S, Mitani Y, Ito R, Nakayama H. Histone acetylation and gastrointestinal carcinogenesis. Ann N Y Acad Sci 2003;983:220-31.

Galvez AF, de Lumen BO. A soybean cDNA encoding a chromatin-binding peptide inhibits mitosis of mammalian cells. Nat Biotechnol 1999;17(5):495-500.

Dia VP, Wang W, Oh VL, de Lumen BO, de Mejia EG. Isolation,purification and characterisation of lunasin from defatted soybean flour and in vitro evaluation of its anti-inflammatory activity. Food Chem 2009;114(1):108-15.

Seber LE, Barnett BW, McConnell EJ, Hume SD, Cai J, Boles K, et al. Scalable purification and characterization of the anticancer lunasin peptide from soybean. PLoS One 2012;7(4):e35409.

Kusmardi, Priosoeryanto BP, Herlina E, Cornain S. Activity of fish oil inhibbtion preneoplasia colon of mice induced azoxymethan adn dextran sodium sulfate. Indonesian Journal of Pharmaceutical Sciences 2014;2(2):151-61.

Banaszkiewicz T. Poultry Feeding Standards. Jablonna, Warszawa, Poland: The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences; 2005.

Bhathena SJ, Velasquez MT. Beneficial role of dietary phytoestrogens in obesity and diabetes. Am J Clin Nutr 2002;76(6):1191-201.

Li FN, Li LL, Yang HS, Yuan XX, Zhang B, Geng MM, et al. Regulation of soy isoflavones on weight gain and fat percentage: Evaluation in a Chinese Guangxi minipig model. Animal 2011;5(12):1903-8.

Algra AM, Rothwell PM. Effects of regular aspirin on long-term cancer incidence and metastasis: A systematic comparison of evidence from observational studies versus randomised trials. Lancet Oncol 2012;13(5):518-27.

Singh Ranger G. The role of aspirin in colorectal cancer chemoprevention. Crit Rev Oncol Hematol 2016;104:87-90.

Narayanan BL, Venkatesan N, Subburaju T, Fathah A. Chemopreventive role of combination of etoricoxib and atrovastatin on colon cancer induced by 1,2-dimethyl hydrazine on rats. Int J Pharm Pharm Sci 2015;7(9):299-303.

Patrignani P, Capone ML, Tacconelli S. Clinical pharmacology of etoricoxib: A novel selective COX2 inhibitor. Expert Opin Pharmacother 2003;4(2):265-84.

Tanaka T. Development of an inflammation-associated colorectal cancer model and its application for research on carcinogenesis and chemoprevention. Int J Inflam 2012;2012:658786.

Garcia-Albeniz X, Chan AT. Aspirin for the prevention of colorectal cancer. Best Pract Res Clin Gastroenterol 2011;25(4-5):461-72.

Messina MJ. Legumes and soybeans: Overview of their nutritional profiles and health effects. Am J Clin Nutr 1999;70 3 Suppl:439S-50.

Soni N, Pardasani KR, Mujwar S. Insilico analysis of dietary agents as anticancer inhibitors of insulin like growth factor 1 receptor (1GF1R). Int J Pharm Pharm Sci 2015;7(9):191-6.

Mahmoud AM, Yang W, Bosland MC. Soy isoflavones and prostate cancer: A review of molecular mechanisms. J Steroid Biochem Mol Biol 2014;140:116-32.

de Lumen BO. Lunasin: A cancer-preventive soy peptide. Nutr Rev 2005;63(1):16-21.

Dwarakanath BS, Verma A, Bhatt AN, Parmar VS, Raj HG. Targeting protein acetylation for improving cancer therapy. Indian J Med Res 2008;128(1):13-21.

Dalvai M, Bystricky K. The role of histone modifications and variants in regulating gene expression in breast cancer. J Mammary Gland Biol Neoplasia 2010;15(1):19-33.

Dia VP, Gonzalez de Mejia E. Lunasin potentiates the effect of oxaliplatin preventing outgrowth of colon cancer metastasis, binds to a5ß1 integrin and suppresses FAK/ERK/NF-?B signaling. Cancer Lett 2011;313(2):167-80.

Galvez AF, Chen N, Macasieb J, de Lumen BO. Chemopreventive property of a soybean peptide (lunasin) that binds to deacetylated histones and inhibits acetylation. Cancer Res 2001;61(20):7473-8.

Jeong HJ, Park JH, Lam Y, de Lumen BO. Characterization of lunasin isolated from soybean. J Agric Food Chem 2003;51(27):7901-6.

Jeong HJ, Lam Y, de Lumen BO. Barley lunasin suppresses ras-induced colony formation and inhibits core histone acetylation in mammalian cells. J Agric Food Chem 2002;50(21):5903-8.

Jeong HJ, Jeong JB, Kim DS, Park JH, Lee JB, Kweon DH, et al. The cancer preventive peptide lunasin from wheat inhibits core histone acetylation. Cancer Lett 2007;255(1):42-8.

Jeong JB, Jeong HJ, Park JH, Lee SH, Lee JR, Lee HK, et al. Cancer-preventive peptide lunasin from Solanum nigrum L. inhibits acetylation of core histones H3 and H4 and phosphorylation of retinoblastoma protein (Rb). J Agric Food Chem 2007;55(26):10707-13.

Hernández-Ledesma B, Hsieh CC, de Lumen BO. Relationship between lunasin’s sequence and its inhibitory activity of histones H3 and H4 acetylation. Mol Nutr Food Res 2011;55(7):989-98.

Balasubramanyam K, Swaminathan V, Ranganathan A, Kundu TK. Small molecule modulators of histone acetyltransferase p300. J Biol Chem 2003;278(21):19134-40.

Balasubramanyam K, Altaf M, Varier RA, Swaminathan V, Ravindran A, Sadhale PP, et al. Polyisoprenylated benzophenone, garcinol, a natural histone acetyltransferase inhibitor, represses chromatin transcription and alters global gene expression. J Biol Chem 2004;279(32):33716-26.

Balasubramanyam K, Varier RA, Altaf M, Swaminathan V, Siddappa NB, Ranga U, Kundu TK. Curcumin, a novel p300/CREB-binding protein-specific inhibitor of acetyltransferase, represses the acetylation of histone/nonhistone proteins and histone acetyl transferase dependent chromatin transcription. J Biol Chem 2004;279(49):51163-71.

Dia VP, Gonzalez de Mejia E. Lunasin induces apoptosis and modifies the expression of genes associated with extracellular matrix and cell adhesion in human metastatic colon cancer cells. Mol Nutr Food Res 2011;55(4):623-34.

Dia VP, Gonzalez de Mejia E. Lunasin potentiates the effect of oxaliplatin preventing outgrowth of colon cancer metastasis, binds to a5ß1 integrin and suppresses FAK/ERK/NF-?B signaling. Cancer Lett 2011;313(2):167-80.

Dia VP, Mejia EG. Lunasin promotes apoptosis in human colon cancer cells by mitochondrial pathway activation and induction of nuclear clusterin expression. Cancer Lett 2010;295(1):44-53.

Dittmar T, Heyder C, Gloria-Maercker E, Hatzmann W, Zänker KS. Adhesion molecules and chemokines: The navigation system for circulating tumor (stem) cells to metastasize in an organ-specific manner. Clin Exp Metastasis 2008;25(1):11-32.

Dia VP, de Mejia EG. Potential of lunasin orally-administered in comparison to intraperitoneal injection to inhibit colon cancer metastasis in vivo. J Cancer Ther 2013;4(6B):34-43.

Brownschidle AM. The effect of soy protein on colon carsinogenesis in vitro and in vivo. Thesis in Food Science, The Pennsylvania State University; 2011.

Published

01-04-2017

How to Cite

Amalia, A. W., KUSMARDI, B. Elya, and A. . ARSIANTI. “INHIBITION OF CARSINOGENESIS BY SEED AND SOYBEAN MEAL EXTRACT IN COLON OF MICE: APOPTOSIS AND DYSPLASIA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 10, no. 4, Apr. 2017, pp. 123-8, doi:10.22159/ajpcr.2017.v10i4.16276.

Issue

Section

Original Article(s)

Most read articles by the same author(s)

<< < 1 2