MEAL INDUCED OXIDATIVE STRESS LEVELS IN CONTROLLED AND POORLY CONTROLLED DIABETES


Sulekha Sinha, Poornima A Manjrekar, Anupama Hegde, Rukmini Ms

Abstract


Objective:  The postprandial metabolic derangements are accentuated in type-2 diabetes and are important risk factors for cardiovascular disease since they induce oxidative stress and endothelial dysfunction. Aim of the study was to analyse meal induced oxidative stress levels in controlled and poorly controlled diabetes.

Methods: Total 60 type 2 diabetic patients on oral hypoglycemics of duration 5-15 years were divided into two groups based on HbA1c values (£7.5% for controlled /Group-1 and ³7.6% for poorly controlled/ Group-2). They were assayed for serum/plasma glucose, thiobarbituric acid reacting substances (TBARS) and oxidised LDL (ox-LDL) parameters both in fasting and 2 hrs post meal in both the groups. Statistical analysis was done using independent t test between the groups and paired t test within each group.

Results: The Postprandial TBARS was found significantly higher in both the groups, whereas no significant difference between the two groups. The ox-LDL levels were found similar at two points of time in both the groups.

Conclusion: An exaggerated postprandial oxidative stress levels are associated with diabetes and its complications including endothelial dysfunction.

 

 


Keywords


TBARS, ox-LDL, HbA1c, Diabetes

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References


Sulekha S, Manjrekar PA, Hegde A, Shenoy R. Meal induced dyslipidemia in Type 2 diabetes mellitus. Biomedicine 2014;34(1):137-41.

Jayesh BD, Snehal NM, Archana RJ. Diabetic nephropathy-genesis, prevention and treatment. Int J Pharm Pharm 2014;6(9):42-7.

Mosab A, Aisha S, Osama A. The association of Type 2 diabetes with obesity and other factors: In multinational community. Int J Pharm Pharm 2014;6(9):257-60.

Nesto RW. Beyond low density lipoprotein: Addressing the atherogenic lipid triad in Type 2 diabetes mellitus and the metabolic syndrome. Am J Cardiovasc Drugs 2005;5(6):379-87.

Suri RK, Guptha MM, Chakkravarthi AK. Hyperlipidemia and vascular populations of diabetes mellitus. J Assoc Physicians India 1979;27:505.

Wojciench W, Jean N, Anne P. Optimized steps in fluorometric determination of thiobarbituric acid reacting substances in serum: Importance of extraction pH and influence of sample preservative and storage. Clin Chem 1993;39(12):2522-6.

Poornima K, Cariappa M, Asha K, Kedilaya HP, Nandini M. Oxidant and antioxidant status in vegetarians and fish eaters. Indian J Clin Biochem 2003;18(2):197-205.

Wasowicz W, Neve J, Peretiz A. Optimized steps in fluorometric determination of thiobarbituric acid reactive substances in serum: Importance of extraction, pH and infuences of sample preservation and storage. Clin Chem 1993;39:2522-6. 9. Scoccia AE, Molinuevo MS, McCarthy AD, Cortizo AM. A simple method to assess the oxidative susceptibility of low density lipoproteins. BMC Clin Pathol 2001;1(1):1.

Wieland H, Seidel D. A simple specific method for precipitation of low density lipoproteins. J Lipid Res 1983;24(7):904-9.

Sacks DB. Correlation between hemoglobin A1c (HbA1c) and average blood glucose: Can HbA1c be reported as estimated blood glucose concentration? J Diabetes Sci Technol 2007;1(6):801-3.

Múnera-Jaramillo MI, Restrepo-Lozada MA, Gómez-Bahamón LM, Mesa-Suarez Ddel R, Ramirez-Puerta BS. Glycosylated haemoglobin A1c compared to fasting plasma glucose in outpatients referred to a medical laboratory. Rev Salud Publica (Bogota) 2011;13(6):980-9.

Sikaris K. The correlation of hemoglobin A1c to blood glucose. J Diabetes Sci Technol 2009;3(3):429-38.

Kikuchi K, Nezu U, Shirakawa J, Sato K, Togashi Y, Kikuchi T, et al. Correlations of fasting and postprandial blood glucose increments to the overall diurnal hyperglycemic status in Type 2 diabetic patients: Variations with levels of HbA1c. Endocr J 2010;57(3):259-66.

Ceriello A, Bortolotti N, Motz E, Pieri C, Marra M, Tonutti L, et al. Meal-induced oxidative stress and low-density lipoprotein oxidation in diabetes: The possible role of hyperglycemia. Metabolism 1999;48(12):1503-8.

Saxena R, Madhu SV, Shukla R, Prabhu KM, Gambhir JK. Postprandial hypertriglyceridemia and oxidative stress in patients of Type 2 diabetes mellitus with macrovascular complications. Clin Chim Acta 2005;359(1-2):101-8.

Anderson RA, Evans LM, Ellis GR, Khan N, Morris K, Jackson SK, et al. Prolonged deterioration of endothelial dysfunction in response to postprandial lipaemia is attenuated by vitamin C in Type 2 diabetes. Diabet Med 2006;23(3):258-64.

Anderson RA, Evans LM, Ellis GR, Graham J, Morris K, Jackson SK, et al. The relationship between post-prandial lipaemia, endothelial function and oxidative stress in healthy individuals and patients with Type 2 diabetes. Atherosclerosis 2001;154(2):475-83.

Tushuizen ME, Nieuwland R, Scheffer PG, Sturk A, Heine RJ, Diamant M. Two consecutive high-fat meals affect endothelial-dependent vasodilation, oxidative stress and cellular microparticles in healthy men. J Thromb Haemost 2006;4(5):1003-10.

Diwadkar VA, Anderson JW, Bridges SR, Gowri MS, Oelgten PR. Postprandial low-density lipoproteins in Type 2 diabetes are oxidized more extensively than fasting diabetes and control samples. Proc Soc Exp Biol Med 1999;222(2):178-84.

Wallace JP, Johnson B, Padilla J, Mather K. Postprandial lipaemia, oxidative stress and endothelial function: A review. Int J Clin Pract 2010;64(3):389-403.

Steinberg D. Low density lipoprotein oxidation and its pathobiological significance. J Biol Chem 1997;272(34):20963-6.




About this article

Title

MEAL INDUCED OXIDATIVE STRESS LEVELS IN CONTROLLED AND POORLY CONTROLLED DIABETES

Keywords

TBARS, ox-LDL, HbA1c, Diabetes

DOI

10.22159/ajpcr.2017.v10i6.17814

Date

01-06-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 6 June 2017 Page: 105-107

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

27 Views | 22 Downloads

Authors & Affiliations

Sulekha Sinha
Department of Biochemistry, IQ City Medical College, Durgapur - 713 206, West Bengal, India
India

Poornima A Manjrekar
Department of Biochemistry, Kasturba Medical College, Mangalore - 575 004, Karnataka, India
India

Anupama Hegde
Department of Biochemistry, Kasturba Medical College, Mangalore - 575 004, Karnataka, India.
India

Rukmini Ms
2Department of Biochemistry, Kasturba Medical College, Mangalore - 575 004, Karnataka, India.
India


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