STUDY ON THE ANTIBACTERIAL ACTIVITY OF FRUIT EXTRACTS OF KLUTUK BANANA (MUSA BALBISIANA COLLA) AGAINST SHIGELLA DYSENTERIAE ATCC 13313


Sriagung Fitri Kusuma, Soraya R Mita, Indah Firdayani, Resmi Mustarichie

Abstract


Objective: The purpose of this study is to determine the antibacterial activity of Klutuk Banana (Musa balbisiana colla) fruit extracts against Shigella dysenteriae ATCC 13313 and the amount of potassium to the discovery of anti-dysentery drug candidates.

Methods: The simplisia of Klutuk banana fruit was extracted with ethanol using a maceration method. The phytochemical screening of ethanol extract was performed using standard procedures. Determination thin layer chromatography (TLC) profile of the extract was performed using a thin layer plate. The antibacterial activity was investigated using agar well diffusion technique. The minimum inhibitory concentrations (MIC) were determined by a serial microdilution method, whereas the minimum bactericidal concentration (MBC) was done by subculturing the MIC result onto agar medium. Potassium levels of the extract were carried out quantitatively using atomic absorption spectrophotometry.

Results: The phytochemical analysis revealed the presence of flavonoids, polyphenols, tannins, monoterpenoid and sesquiterpenoids, quinones, and saponins. The TLC results prove the existence of flavonoids in the tested extract. The content of secondary metabolites that can act as an antibacterial, strengthen the antibacterial activity of ethanol extract of Klutuk banana against S. dysenteriae 13313 with MBC values in the range of 5-10%w/v. Potassium levels in the ethanol extract of Klutuk banana fruits contain potassium as much as 2.919% (29 190 ppm).

Conclusion: It can be concluded that the ethanol extract of Klutuk banana fruits is more potent as antibacterial against S. dysenteriae than as potassium supplier in hypokalemia therapy.


Keywords


Klutuk, Bananas, Shigella dysenteriae 13313, Antibacterial, Potassium.

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About this article

Title

STUDY ON THE ANTIBACTERIAL ACTIVITY OF FRUIT EXTRACTS OF KLUTUK BANANA (MUSA BALBISIANA COLLA) AGAINST SHIGELLA DYSENTERIAE ATCC 13313

Keywords

Klutuk, Bananas, Shigella dysenteriae 13313, Antibacterial, Potassium.

DOI

10.22159/ajpcr.2017.v10i7.18561

Date

01-07-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 7 July 2017 Page: 220-223

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

86 Views | 114 Downloads

Authors & Affiliations

Sriagung Fitri Kusuma
Department of Pharmaceutical Biology, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia.
Indonesia

Soraya R Mita
Department of Pharmaceutical Biology, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia.
India

Indah Firdayani
Department of Pharmaceutical Biology, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia.
India

Resmi Mustarichie
Department of Pharmaceutical Biology, Faculty of Pharmacy, Padjadjaran University, Sumedang, West Java, Indonesia.
India


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