CHARACTERISTICS AND ALOPECIA ACTIVITY OF PAKIS GAJAH (ANGIOPTERIS EVECTA (G.FORST) HOFFM.) GROWING IN GALUNGGUNG MOUNTAINSIDE, WEST JAVA
Â Objective: This study aims to determine characteristics and alopecia activity of Pakis Gajah (Angiopteris evecta (G.Forst) Hoffm.) growing in Galunggung mountainside, West Java.
Methods: A. evecta was collected, determined and extracted using ethanol 95% by maceration. The extract was characterized based on Farmakope Indonesia method. Phytochemical screening was applied based on Farnsworthâ€™s method. Its alopecia activity was determined by Tanaka method on male Angora rabbits.
Results: The results showed that A. evecta simplicia had dried shrinkage characteristics of 3.35%; ash content 4.73%; water soluble sari content 20.11%; ethanol soluble concentration 5.94%; density 0.820; pH of extract 5.33; microchemical examination with H2SO4 gives a dark brown color, with 2N NaOH giving a bright yellow color, with the alkaline solution giving fluorescence, with FeCl3 giving a greenish-green color; its chemical contents were polyphenols, flavonoid, steroids, triterpenoids, quinones, monoterpenoid, and sesquiterpenoids. Alopecia activity showed that the water fraction and n-hexane had hair growth stimulating activity and water fraction was the fraction that had the most excellent activity, while ethyl acetate did not have such activities. The alopecia results were compared with minoxidil standards.
Conclusion: These results indicated that the water fractions A. evecta had the most alopecia activity on research animal. Our results suggest before it can be used in humans, it is necessary to do further research on the bald volunteers.
2. Stough D, Stenn K, Haber R, Parsley WM, Vogel JE, Whiting DA, et al. Psychological effect, pathophysiology, and management of androgenetic alopecia in men. Mayo Clin Proc 2005;80:1316-22.
3. Jain R, Monthakantirat O, Tengamnuay P, De-Eknamkul W. Identification of a new plant extract for androgenic alopecia treatment using a non-radioactive human hair dermal papilla cell-based assay. BMC Complement Altern Med 2016;16:18.
4. Kuncari ES, Iskandarsyah I, Praptiwi P. Test irritation and activity of white rat hair growth: Apigenin gel and celery gel effect (Apium graveolens L.), Media Litbangkes 2015;25(1):15-22.
5. Mustarichie R, Indriyati W, Mukmin A, Ramdhani D. Activity of Angiopteris evecta for baldness treatment. J Chem Pharm Res 2016;8(5):821-30.
6. Amin J, Simamora EL, Anwar E, Djajadisastra J. Green tea (Camellia sinensis L.) ethanolic extract as hair tonic in nutraceutical: Physical stability, hair growth activity on rats, and safety test. Int J Pharm Pharm Sci 2014;6(5):94-9.
7. Arnida A, Wahyono W, Mustofa M, Asmahsusidarti R. In vitro antiplasmodial activity of ethanol extracts of Borneo medicinal plants (Hydrolea spinosa, Ampelocissus rubiginosa, Uraria Crinite, Angiopteris evecta. Int J Pharm Pharm Sci 2015;7(5):72-5.
8. Tanaka S, Saito M, Tabata M. Bioassay of crude drugs for hair growth promoting activity in mice by a new simple method. Planta Med 1980;40:84-90.
9. Depkes RI. Farmakope Indonesia. 4th ed. Jakarta: Depkes, RI; 1995.
10. Departemen Kesehataan RI. Farmakope Herbal Indonesia. 1st ed. Jakarta: Departemen Kesehataan RI; 2015. p. 17-8.
11. Gatbonton GL, de Jesus AP, Lorenzo KM, Uy MM. Soxhlet Extraction of Philippine Avocadro Fruit Pulp Variety 240, Presented at the Research Congress 2013 De La Salle University Manila March, 7-9; 2013.
12. Marnoto T, Haryono G, Gustinah D, Putra FA. Tannin extraction as a natural dye from the Putrimalu plant (Mimosa pudica) using organic solvent, Reaktor 2012;14(1):39-45.
13. Farnsworth NR. Biological and phytochemical screening of plants. J Pharm Sci 1966;55(3):225-76.
14. Harborne AJ. Phytochemical Methods a Guide to Modern Techniques of Plant Analysis, Metode Fitokimia Translated by Kosasih Padmawinata dan Iwang Soediro. Bandung: Penerbit ITB; 1987.
15. Mismawati A, Suwannaket CS, Mingvanish W, Kuspradini H, Kusuma IW, et al. Phytochemical Screening and Bioactivity of Angiopteris evecta leaves from East Kalimantan, Pure and Applied Chemistry International Conference 2015 (PACCON2015). Available from: https://www.researchgate.net/publication/286154161.
16. Gracelin DH, de Britto AJ, Kumar PB. Antibacterial screening of a few medicinal ferns against antibiotic resistant phyto pathogen. Int J Pharm Sci Res 2012;3(3):868-73.
17. Kale MV. Phytochemical analysis of whole plant extracts of Angiopteris heliferiana. Res J Life Bioinform Pharm Chem Sci 2015;1(3):139-42.
18. de Britto AJ, Gracelin DH, Kumar PB. Study on potential biocontrol agent-Angiopteris evecta (Forst) Hoff. Against Xanthomonas campestris. Eur J Mol Biol Biochem 2014;1(5):192-5.
19. Koch J, Shiveler G. Design Principles for Liquid-Liquid Extraction, CEP Magazine; 2015. Available from: https://www.aiche.org/resources/ publications/cep/2015/november/design-principles-liquid-liquid-extraction.
20. Marchaban JC, Soegihardjo S, Kumarawati FE. Test of Randu Leaf Activity (Ceiba pentandra Gaertn.) as a Hair Grower (Uji Aktifitas Daun Randu (Ceiba Pentandra Gaertn.) Sebagai Penumbuh Rambut, UGM, Yogyakarta; 2007. Available from: https://www.mot.farmasi. ugm.ac.id/files/41Daun%20randu_marchaban.pdf.
21. Robinson T. High Organic Plant Content (Kandungan Organik Tumbuhan Tinggi). Bandung: Penerbit ITB; 1995.
22. Sitompul S. Content of Polyphenol Compounds in Aloe Vera Plants, Mimba Leaves and Noni Fruit Dregs (Kandungan Senyawa Polifenol Dalam Tanaman Lidah Buaya, Daun Mimba dan Ampas Buah Mengkudu). Bogor: Prosiding Temu Teknis Fungsional Non Peneliti; 2002. p. 48-55.
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.