CABBAGELEAF EXTRACT(BRASSICA OLERACEA VAR. CAPITATA ALBA) AS A HERBAL MEDICINE FOR LEUCORRHEA

  • Ami Tjitraresmi Department of Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
  • Sri Agung Fitri Kusuma Department of Faculty of Pharmacy, Universitas Padjadjaran, Indonesia
  • Dewi Rusmiati Department of Faculty of Pharmacy, Universitas Padjadjaran, Indonesia

Abstract

Objective:This study was aimed to examine antifungal activity of cabbage leaf extracts against Candida albicans, determined the minimum inhibitory concentration (MIC), analyzed the comparative value of the extracts with ketoconazole and studied the fastest contact time to eliminate Candida albicans.

 Methods: The extraction of fresh cabbage leaf was done using the maceration method. The antifungal activity test and its comparative analysis against ketokonazole were assessed using the agar diffusion method. The extracts were tested for determining minimum inhibitory concentration value (MIC) using solid medium. While the fastest contact time test was performed using turbidimetric method.

Results: Based on its inhibitory diameter, cabbage leaf extracts gave potent antifungal activity against Candida albicans. The MIC concentrations of testing extracts were various ranged from 1.50 to 1.75%w/v. In a comparative analysis of the extracts with ketoconazole, showed that ketoconazole gave greater antifungal activity than the extract at the same concentration. Cabbage leaf extract with concentration 2.5% gave the fastest contact time (2.5 minutes) for eliminating Candida albicans, while Cabbage leaf extract with concentration 0.4% gave the longest contact time (15 minutes) for eliminating Candida albicans.

Conclusion:Cabbage leaf extract has a potential antifungal activity against Candida albicans, and prospective to be developed as a topical herbal medicine for treating candidiasis.

 

 

Keywords: Brassica oleraceae var. capitata alba, Cabbage, Candida abicans.

References

1. Wozniak KL, Floyd LW, Paul LF. Candida-Specific Antibodies during Experimental Vaginal Candidiasis in Mice. Infect. Immun 2002; 70 Suppl10: 5790-9.

2. Ryley JF, Wilson RG, Barrett-Bee KJ. Azole resistance in Candida albicans, Sabouraudia. Oxf. J 1984; 22 Suppl1:53-63.

3. McCullough MJ, Ross BC, Reade PC. Candida albicans: a review of its history, taxonomy, epidemiology, virulence attributes, and methods of strain differentiation. Int. J. Oral Surg1996; 25 Suppl1: 136–44.

4. Barchiesi F, Morbiducci V, Ancarani F, Scalise G. Emergence of oropharyngeal candidiasis caused by non-albicans species of Candida in HIV-infected patients. Eur. J. Epidemiol1993; 9 Suppl1: 455–6.

5. Powderly WG, Robinson K, Keath EJ. Molecular epidemiology of recurrent oral candidiasis in human immunodeficiency virus-positive patients: evidence for two patterns of recurrence. J. Infect. Dis1993; 168 Suppl 1: 463–6.

6. Vazquez JA. Options for the management of mucosal candidiasis in patients with AIDS and HIV infection. Pharmacotherapy 1999; 19 Suppl 1: 76–87.

7. Miyazaki T, Yoshitsugu M, Koichi I, Kakeya, Shunichi M., John EB. Fluconazole treatment is effective against a Candida albicans erg3/erg3 mutant in vivo despite in vitro resistance. Antimicrob. Agents Chemother 2006; 50 Suppl 2: 580-6.

8. Sheehan DJ, Christopher AH, Carol MS. Current and emerging azole antifungal agents.Clin. Microbiol. Rev 1999; 12 Suppl 1: 40-79.

9. Hiratani T, Yamaguchi H. Cross-resistance of Candida albicans to several different families of antifungals with ergosterol biosynthesis-inhibiting activity. Jpn J. Antibiot1994; 47 Suppl2:125-8.

10. Naglik JR, Stephen JC, Bernhard H. Candida albicans secreted aspartyl proteinases in virulence and pathogenesis. Microbiol. Mol. Biol. Rev 2003; 67 Suppl 3: 400-28.

11. Zeichner LO, John HR, Peter GP, Richard JH, Robert AL, Harold W. H. Antifungal susceptibility survey of 2,000 bloodstream Candida isolates in the United States. Antimicrob. Agents Chemother 2003; 47 Suppl 10: 3149-54.

12. Charlier C, Hart E, Lefort A. Fluconazole for the management of invasive candidiasis: where do we stand after 15 years. J AntimicrobChemother 2006; 57 Suppl 3: 384-410.


13. Hu SH, Wang JC, Kung HF, Wang JT, Lee WL, Yang YH. Antimicrobial effect of extracts of cruciferous vegetables. Kaohsiung J Med Sci 2004; 20 Suppl 12: 591-9.

14. Suryani, Y. Penggunaanbakteriasamlaktatdalamfermentasi sauerkraut sebagaialternatifpengawetandanpengolahanKubis (Brassica oleraceavarcapitata f. alba). Master Theses. Bogor: IPB; 2004.

15. Shiva RB, Jung-Ho K. Chemical composition and antioxidant activity in different tissues of Brassica Vegetables. Mol 2015; 20 Suppl1: 1228-43.

16. BalouiriM ,Moulay S, Saad K.I. Methods for in vitro evaluating antimicrobial activity: A review. J. Pharm. Anal 2016; 6 Suppl2: 71–9.

17. Agarwal RK, Gupta S, Mittal G, Khan F, Roy S, AgarwalInt A. Antifungal susceptibility testing of dermatophytes by agar based disk diffusion Method. J CurrMicrobiol App Sci 2015; 4 Suppl3: 430-6.

18. Subhas CM, Harsha R, Dinesha R, Thammanna GSS. Antibacterial activity of Coleus aromaticus leaves. Int J Pharm Sci. 2010; 2 Suppl 1: 63-6.
Statistics
244 Views | 293 Downloads
Citatons
How to Cite
Tjitraresmi, A., S. A. F. Kusuma, and D. Rusmiati. “CABBAGELEAF EXTRACT(BRASSICA OLERACEA VAR. CAPITATA ALBA) AS A HERBAL MEDICINE FOR LEUCORRHEA”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 10, no. 14, May 2017, pp. 39-41, doi:10.22159/ajpcr.2017.v10s2.19483.
Section
Original Article(s)