THE ROLE OF INTERLEUKIN-4 GENE PROMOTER POLYMORPHISMS IN GRAVES’ DISEASE

  • RAVEINAL RAVEINAL Department of Internal Medicine, Allergic and Immunology Subdivision, Dr. M. Djamil General Hospital/Faculty of Medicine, Andalas University, Padang, Indonesia.
  • ERYATI DARWIN Department of Histology and Immunology, Faculty of Medicine, Andalas University, Padang, Indonesia.
  • EVA DECROLI Department of Internal Medicine, Endocrinology and Metabolic Subdivision, Dr. M. Djamil General Hospital/Faculty of Medicine, Andalas University, Padang, Indonesia.
  • JAMSARI JAMSARI Department of Agriculture, Faculty of Agriculture, Andalas University, Padang-Indonesia.

Abstract

Objective: This study was conducted to prove the role of interleukin-4 gene promoter polymorphisms in Graves’ disease patients in M Djamil General Hospital Padang, Indonesia.


Methods: This study was conducted from August 2015 until December 2015 in the Internal Medicine Department in Dr. M. Djamil Hospital, Padang, West Sumatera, Indonesia. This study involved 15 patients with Graves’ disease and 15 normal subjects. We examined that IL-4 promoter gene polymorphism was examined with PCR.


Results: Sequencing examination on IL-4 gene promoter resulted in 2 Single Nucleotide Polymorphism (SNP) motifs, which is rs2243250 and rs2070847. IL-4 SNP gene promoter polymorphisms rs2243250 and rs2070847 were found in both patient and control groups. TT is homozygous SNP polymorphisms. CT is heterozygous SNP polymorphisms. CC is wild type or no mutation SNP polymorphisms. Based on statistical tests, no difference in rs2243250 and rs2070847 SNP polymorphisms was found between patient and control group (p > 0.05).


Conclusion: This study observed no difference in interleukin-4 gene promoter polymorphism between Graves’ disease patients and control group.

Keywords: IL-4, Gene promoter, Polymorphism, Graves' disease

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RAVEINAL, R., E. DARWIN, E. DECROLI, and J. JAMSARI. “THE ROLE OF INTERLEUKIN-4 GENE PROMOTER POLYMORPHISMS IN GRAVES’ DISEASE”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 13, no. 11, Aug. 2020, pp. 73-75, doi:10.22159/ajpcr.2020.v13i11.39151.
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