USING HAIR GROWTH ACTIVITY, PHYSICAL STABILITY, AND SAFETY TESTS TO STUDY HAIR TONICS CONTAINING ETHANOL EXTRACT OF LICORICE (GLYCYRRHIZA GLABRA LINN.)


Sheila Meitania Utami, Joshita Djajadisastra, Fadlina Chany Saputri

Abstract


Objective: The purpose of this study was to determine the safety, physical stability, and hair growth activity of ethanol extract of licorice.
Methods: In this study, 2.5%, 5%, and 10% licorice extract was formulated into a hair tonic as a tonic is easier to use and is not sticky like a semisolid
dosage. The hair growth activity test was conducted by rubbing the hair tonic preparations on rabbit’s backs; subsequently, the hair length, hair
thickness, hair weight, and hair density were measured. Quantitative analysis of glycyrrhizic acid from the licorice ethanol extract with a ultraviolet
spectrophotometer showed a level of about 156.65 mg/g or 15.665%. The physical stability test was performed on samples of the tonic stored at low
(4±2°C), room (25±2°C), and high (40±2°C) temperature, and a cycling test was also performed. The safety test was performed using an eye irritation
test that employed the Hen’s egg test–chorioallantoic membrane (HET-CAM) method and a skin irritation test that employed the patch test method.
Results: The hair tonics containing 5% and 10% licorice extract had an equivalent activity of hair growth and even better than the positive control
containing 2% minoxidil. The physical stability test showed that the licorice extract hair tonic has good physical stability. The results of the safety test
showed no skin irritation, whereas the HET-CAM test showed that the hair tonic containing licorice extract showed mild eye irritation.
Conclusions: Licorice ethanol extract hair tonic solutions in concentrations of 2.5%, 5%, and 10% had hair growth activity similar to that of the
positive control (minoxidil). They have a good physical and chemical stability, also safe for topical use, except the 2.5% licorice ethanol extract hair
tonic solution which caused mild eye irritation.


Keywords


Licorice, Hair growth activity, Glycyrrhizic acid, Mild irritation.

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About this article

Title

USING HAIR GROWTH ACTIVITY, PHYSICAL STABILITY, AND SAFETY TESTS TO STUDY HAIR TONICS CONTAINING ETHANOL EXTRACT OF LICORICE (GLYCYRRHIZA GLABRA LINN.)

Keywords

Licorice, Hair growth activity, Glycyrrhizic acid, Mild irritation.

DOI

10.22159/ijap.2017.v9s1.20_25

Date

30-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 44-48

Online ISSN

0975-7058

Statistics

13 Views | 23 Downloads

Authors & Affiliations

Sheila Meitania Utami
Department of Herbal, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Joshita Djajadisastra
Department of Herbal, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Fadlina Chany Saputri
Department of Herbal, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia


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