FORMULATION AND EVALUATION OF TWO CELASTROL NANOEMULSIONS PREPARED FROM TWO OILS: ISOPROPYL MYRISTATE AND VIRGIN COCONUT OIL

Authors

  • NUR ALAM ABDULLAH Student of Pharmaceutical Sciences, Faculty of Pharmacy, University of Indonesia
  • MAHDI JUFRI Department of Pharmaceutical Technology and Drug Development, Faculty of Pharmacy, University of Indonesia https://orcid.org/0000-0003-3084-5285
  • ABDUL MUN’IM Department of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, University of Indonesia
  • FADLINA CHANY SAPUTRI Departemen Pharmacology and Pharmacokinetics, University of Indonesia, Depok, 16424, University of Indonesia

DOI:

https://doi.org/10.22159/ijap.2022v14i2.43509

Keywords:

Nanoemulsions Celastrol IPM and VCO

Abstract

Objective: Celastrol which is classified as BCS 4, needs to be developed into a nanoemulsion formula for a stable and good formulation. The aim of the study was to determine the in vitro penetration ability and adsorption efficiency (EE) between two different base oils, namely Isopropyl myristate (IPM) and virgin coconut. oil (VCO).

Methods: Two celastrol nanoemulsion formulas were prepared by high energy method using High share homogenizer (HSH) at 15,000 rpm for 15 minutes, using different oil-based components, F1 IPM and F2 VCO. Particle size, polydispersity index (PDI), D90, zeta potential, and morphology of nanoemulsions was evaluated. In vitro studies by Franz diffusion cell test method determined the difference.

Results: The results showed that celastrol can be formulated well with a ternary ratio of 5:45:50 for IPM and 20:30:50 for VCO. The absorption efficiency test for celestrol levels was 96.49%±2.72 for IPM and 76.53%±1.19 for VCO. The mean particle size, PDI, and zeta potential were 70.81±0.20 nm, 0.1±0.03, and 50.2±0.60 mV, for VCO and 186.23±3, respectively. 12 nm, 0.2±0.07, and 45.5±1.10 mV for HDI. Spherical morphology < 200 nm. Franz diffusion in vitro at 20 and 24 hours, celastrol is well penetrated at levels of 2.4 g/ml gram and 2.5 g/ml for HDI and at 2.0 g/ml gram and 2.4 g/ml, respectively. ml/gram for VCO.

Conclusion: Celastrol was successfully developed into nanoemulsions using IPM or VCO, particle size <200 nm, and stable spherical shape.

Downloads

Download data is not yet available.

References

X. Peng et al., “Optimized preparation of celastrol-loaded polymeric nanomicelles using rotatable central composite design and response surface methodology, J. Biomed. Nanotechnol., 2012 ; 491–499. doi: 10.1166/jbn.2012.1398.

Q. Kang et al., “Transdermal delivery system of nanostructured lipid carriers loaded with Celastrol and Indomethacin: optimization, characterization and efficacy evaluation for rheumatoid arthritis. 2018. doi: 10.1080/21691401.2018.1503599.

Y. Singh et al., “Nanoemulsion: Concepts, development and applications in drug delivery,” J.. 2017 ; 28–49. doi: 10.1016/j.jconrel.2017.03.008.

S. A. Chime, F. C. Kenechukwu, and A. A. Attama, “Nanoemulsions — Advances in Formulation, Characterization and Applications in Drug Delivery. 2014. doi: 10.5772/58673.

R. C. Rowe, P. J. Sheskey, and M. E. Quin, “Handbook of Pharmaceutical Excipients,” 2009.

A. Arellano, S. Santoyo, C. Martín, and P. Ygartua, “Influence of propylene glycol and isopropyl myristate on the in vitro percutaneous penetration of diclofenac sodium from carbopol gels,. 1999 ; 129–135. doi: 10.1016/S0928-0987(98)00010-4.

Nanotechnology in Drug Delivery. 2009 : 663. doi: 10.5772/51384.

S. Shafiq-un-Nabi et al., “Formulation development and optimization using nanoemulsion technique. 2007 ; 1–6. doi: 10.1208/pt0802028.

Enhanced transdermal permeability of Terbinafine through novelnanoemulgel formulation Development in vitro and in vivocharacterization. 2017 ; 18-28. 10.1016/j.fjps.2017.07.003

H. K. Syed and K. K. Peh, “Identification of phases of various oil, surfactant/co-surfactants and water system by ternary phase diagram 2014 ; 301–309. doi: 10.1109/CEC.2002.1007011.

B. Kesumawardhany and S. R. Mita, “Review Artikel: Pengaruh Penambahan Tween 80 sebagai Enhancer dalm Sediaan Transdermal. 2016 ; 1–13. doi: 10.24198/JF.V14I2.9293.

S. H. Ansari, F. Islam, and M. Sameem, “Influence of nanotechnology on herbal drugs: A Review . 2012;142–146. doi: 10.4103/2231-4040.101006.

M. A. Dos Santos Ramos et al., “Nanotechnology-based drug delivery systems for control of microbial biofilms: A review. 2018 ; 1179–1213. doi: 10.2147/IJN.S146195.

T. P. Sari et al., “Preparation and characterization of nanoemulsion encapsulating curcumin. 2015 ; 540–546. doi: 10.1016/j.foodhyd.2014.07.011.

V. K. Rai, N. Mishra, K. S. Yadav, and N. P. Yadav, “Nanoemulsion as pharmaceutical carrier for dermal and transdermal drug delivery. 2018 ; 203–225. doi: 10.1016/j.jconrel.2017.11.049.

F. Shakeel, S. Baboota, A. Ahuja, J. Ali, and S. Shafiq, “Skin permeation mechanism and bioavailability enhancement of celecoxib from transdermally applied nanoemulsion. 2008 ; 1–11. doi: 10.1186/1477-3155-6-8.

Al-Shaibania AJN, Al-Gburib KMH, Hamraha KTKA, Alridhab AMA. Design and characterization of candesartan cilexetil oral nanoemulsion containing garlic oil. Int J Appl Pharm. 2019;11(6):116–24.

H. Choudhury et al., “Recent Update on Nanoemulgel as Topical Drug Delivery System. 2017 ; 1736–1751, 2017, doi: 10.1016/j.xphs.2017.03.042.

M. Ahmad et al., “Development of a new rutin nanoemulsion and its application on prostate carcinoma PC3 cell line. 2017 ; 810–823. doi: 10.17179/excli2016-668.

A. Azeem et al., “Nanoemulsion Components Screening and Selection: a Technical Note. 2009 ; 69–76, Mar. 2009, doi: 10.1208/s12249-008-9178-x.

M. Chiesa, J. Garg, Y. T. Kang, and G. Chen, “Thermal conductivity and viscosity of water-in-oil nanoemulsions. 2008 ; 7–72, 2008, doi: 10.1016/j.colsurfa.2008.05.028.

M. C. Rave, J. D. Echeverri, and C. H. Salamanca, “Improvement of the physical stability of oil-in-water nanoemulsions elaborated with Sacha inchi oil employing ultra-high-pressure homogenization. 2019. doi: 10.1016/j.jfoodeng.2019.109801.

T. Delmas et al., “How to prepare and stabilize very small nanoemulsions. 2011; 1683–1692, 2011, doi: 10.1021/la104221q.

S. W. Jeong, J. Locat, J. K. Torrance, and S. Leroueil, “Thixotropic and anti-thixotropic behaviors of fine-grained soils in various flocculated systems. 2015 ; 119–125. doi: 10.1016/j.enggeo.2015.07.014.

Maha HL, Sinaga KR, Masfria. Formulation and evaluation of miconazole nitrate nanoemulsion and cream. Asian J Pharm Clin Res. 2018;11(3):319–21.

C. Anwar, “Changes in yield and quality of virgin coconut oil (VCO) at various rotational speeds and length of centrifugation time. 2016 ; 51–60. doi: 10.24198/jt.vol10n2.8.

M. E. Helgeson, “Colloidal behavior of nanoemulsions: Interactions, structure, and rheology,” Curr. Opin. Colloid Interface Sci. 2016 ; 39–50. doi: 10.1016/j.cocis.2016.06.006.

V. Klang, N. B. Matsko, C. Valenta, and F. Hofer, “Electron microscopy of nanoemulsions: An essential tool for characterisation and stability assessment,” Micron. 2012 ; 85–103. doi: 10.1016/j.micron.2011.07.014.

H. Sun et al., “Development and characterization of a novel nanoemulsion drug-delivery system for potential application in oral delivery of protein drugs,” Int. J. Nanomedicine, 2012 ; 5529–5543. doi: 10.2147/IJN.S36071.

M. N. Yukuyama et al., “Olive oil nanoemulsion preparation using high-pressure homogenization and D-phase emulsification – A design space approach,” J. Drug Deliv. Sci. Technol. 2019 ; 622–631. doi: 10.1016/j.jddst.2018.12.029.

V. Klang, N. Matsko, K. Raupach, N. El-Hagin, and C. Valenta, “Development of sucrose stearate-based nanoemulsions and optimisation through γ-cyclodextrin,” Eur. J. Pharm. Biopharm. 2011 ; 58–67, 2011, doi: 10.1016/j.ejpb.2011.01.010.

Z. Ghiasi, F. Esmaeli, M. G. Khansari, M. A. Faramarzi, and A. Amani, “Enhancing analgesic and anti-inflammatory effects of capsaicin when loaded into olive oil nanoemulsion : an in-vivo study Department of Medical Nanotechnology , School of Advanced Technologies in Medicine , Tehran University of Medical Sciences , Tehran ,” Int. J. Pharm. 2019 ; doi: 10.1016/j.ijpharm.2019.01.043.

C. H. Salamanca, A. Barrera-Ocampo, J. C. Lasso, N. Camacho, and C. J. Yarce, “Franz diffusion cell approach for pre-formulation characterisation of ketoprofen semi-solid dosage forms,” Pharmaceutics. 2018 ; 1–10. doi: 10.3390/pharmaceutics10030148.

Published

23-12-2021

How to Cite

ABDULLAH, N. A., JUFRI, M., MUN’IM, A., & SAPUTRI, F. C. (2021). FORMULATION AND EVALUATION OF TWO CELASTROL NANOEMULSIONS PREPARED FROM TWO OILS: ISOPROPYL MYRISTATE AND VIRGIN COCONUT OIL. International Journal of Applied Pharmaceutics, 14(2). https://doi.org/10.22159/ijap.2022v14i2.43509

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 3 > >>