DEVELOPMENT AND IN VITRO PENETRATION TEST OF ETHOSOMAL CREAM-CONTAINING PEGAGAN (CENTELLA ASIATICA) HERBAL EXTRACT
Objective: This study aimed to formulate and characterize ethosome-containing Centella asiatica herbal extract at various concentrations and
evaluate the in vitro penetration ability of an ethosome-containing cream.
Methods: The ethosomes were formulated using a thin-layer hydration method with a rotary vacuum evaporator. The asiaticoside content was
determined by thin-layer chromatography densitometry.
Results: Based on the characterization results, F1 was the best ethosomal formula. F1 had the properties of a spherical shape, entrapment efficiency
of 75.03Â±0.295%, particle size of 94.71Â±2.807 nm, and zeta potential of âˆ’25.067Â±0.814 mV. Therefore, the F1 ethosomes were formulated into a
cream with a stearic acid base. The physical stability and in vitro penetration ability of the ethosomal cream were evaluated using Franz diffusion
cells and then compared to a non-ethosomal cream. Based on the results of the penetration study, the cumulative penetration and flux rates of
the ethosomal cream were superior to those of the non-ethosomal cream. The cumulative penetration depths of the ethosomal and non-ethosomal
creams were 3651.271Â±37.579 and 2873.016Â±36.850 Î¼g/cm2, respectively.
Conclusion: Incorporation of asiaticoside into ethosomes can increase its skin penetration ability.
2. Ministry of Health Republic of Indonesia. Indonesian Materia Medics.
1st ed. Jakarta: Ministry of Health Republic of Indonesia; 1977.
3. Lee JH, Kim HL, Lee MH, You KE, Kwon BJ, Seo HJ, et al. Asiaticoside
enhances normal human skin cell migration, attachment and growth in
vitro wound healing model. Phytomedicine 2012;19:1223-7.
4. Elsaie ML, Baumann LS, Elsaaiee LT. Striae distensae (stretch
marks) and different modalities of therapy: An update. Dermatol Surg
5. Beltrami B, Vassallo C, Berardesca E, Borroni G. Anti-inflammatory,
antimicrobial, comedolytic effects of a topical plant complex treatment
in acne vulgaris: A clinical trial. J Appl Cosmetal 2001;10:11-20.
6. Zhang CZ, Niu J, Chong YS, Huang YF, Chu Y, Xie SY, et al. Porous
microspheres as promising vehicles for the topical delivery of poorly
soluble asiaticoside accelerate wound healing and inhibit scar formation
in vitro and in vivo. Eur J Pharm Biopharm 2016;109:1-3.
7. Ajazuddin , Saraf S. Applications of novel drug delivery system for
herbal formulations. Fitoterapia 2010;81:680-9.
8. Apriani EF, Nurleni N, Nugrahani HN, Iskandarsyah I. Stability
testing of azelaic acid cream based ethosome. Asian J Pharm Clin Res
9. Malvern Instruments. Zetasizer Nano Series User Manual. Ch. 4.
Worcestershire: Malvern Instruments, Ltd.; 2013. p. 9.
10. Thakker DK, Chern WH. Development and validation of in vitro release
tests for semisolid dosage forms-case study. Dissolution Technol
11. Anwar E, Utami TD, Ramadon D. Transfersomal gel containing green
tea (Camellia sinensis L. Kuntze) leaves extract: Increasing in vitro
penetration. Asian J Pharm Clin Res 2017;10:294-8.
12. Nurleni N, Iskandarsyah I, Aulia A. Formulation and penetration
testing of ethosome azelaic acid on abdominal skin white male rats
(Rattus norvegicus) with Franz diffusion cell. Asian J Pharm Clin Res
13. Laouini A, Jaafar MC, Limayem BI, Sfar S, Charcosset C, Fessi H.
Preparation, characterization and applications of liposomes: State of the
art. J Colloid Sci Biotechnol 2012;1:147-68.
14. Dâ€™Souza GG, editor. Liposomes Methods and Protocols. 2nd ed.
New York: Humana Press; 2017.
15. Liu Y, Wen C, Yang Y. Development of ethosome-like catanionic
vesicles for dermal drug delivery. J Taiwan Inst Chem Eng
16. Rakesh R, Anoop KR. Ethosome for transdermal and topical drug
delivery. Int J Pharm Pharm Sci 2012;4:17-24.
17. Wu X, Guy RH. Application of nanoparticles in topical drug delivery in
cosmetics. J Drug Deliv Sci Technol 2009;19:371-84.
18. Ansel HC, Allen LV, Popovich NG, editors. Anselâ€™s Pharmaceutical
Dosage Forms and Drug Delivery Systems. 9th ed. Philadelphia, PA:
Wolters Kluwer; 2011.