PHYSICAL STABILITY AND ANTIOXIDANT ACTIVITY ASSAY OF A NANOEMULSION GEL FORMULATION CONTAINING TOCOTRIENOL


Mentari Mayang Suminar, Mahdi Jufri

Abstract


Objective: Tocotrienols have an antioxidant potential higher than that of tocopherols. Nanoemulsion gel can deliver tocotrienols into the skin to
prevent skin damage caused by free radicals and improve the stability of the dosage form. The present study aimed to determine the physical stability
and antioxidant activity of a nanoemulsion gel formulation containing tocotrienol.
Methods: The tocotrienol nanoemulsion was made using tocotrienols, oleic acid, Tween 80, 96% ethanol, and propylene glycol. The gel base was made
using a carbomer and triethanolamine. A physical stability test was conducted at three different temperatures, namely, low temperature (4±2°C),
room temperature (27±2°C), and high temperature (40±2°C). The antioxidant activity was measured using the 2,2-diphenyl-1-picrylhydrazyl method
for determining inhibitory concentration (IC50) values.
Results: Formula 1 demonstrated the best physical stability, with a pH of 6.2. The droplet size of the tocotrienol nanoemulsion gel was 596 nm, with
a zeta potential value of −27.1 nm. The IC50 of the tocotrienol nanoemulsion gel was 6252.14 ppm.
Conclusion: The nanoemulsion gel formulation retained antioxidant activity and was physically stable for 8 weeks.


Keywords


Antioxidant, Stability, Tocotrienol, Nanoemulsion gel.

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References


Pojsak B, Dahmane R. A review on free radicals and extrinsic skin

aging. Dermatology Research and Practice. Slovenia: Hindawi

Publishing Corporation; 2011.

Cotovio J, Onno L, Justine P, Lamure S, Catroux P. Generation of

oxidative stress in human cutaneous models following in vitro ozone

exposure. Toxicol In Vitro 2001;15(4-5):357-62.

Valacchi G, Sticozzi C, Pecorelli A, Cervellati F, Cervellati C, Maioli E.

Cutaneous responses to environmental stressors. Ann N Y Acad Sci

;1271:75-81.

Papas AM. Antioxidant Status, Diet, Nutrition and Health. London:

CRC Press; 1999.

Rahman T, Hosen I, Islam MM, Shekhar HU. Oxidative stress and

human health. Adv Biosci Biotechnol 2012;3:99-1019.

Devasagayam TP, Tilak JC, Boloor KK, Sane KS, Ghaskadbi SS,

Lele RD. Free radicals and antioxidants in human health: Current status

and future prospects. J Assoc Physicians India 2004;52:794-804.

Burgess CM. Cosmetic Dermatology. Berlin: Springer; 2005.

Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants

and functional foods: Impact on human health. Pharmacogn Rev

;4(8):118-26.

Stojiljković D, Pavlović D, Arsić I. Oxidative stress, skin aging, and

antioxidant therapy. J Fac Med Nis 2014;31(4):207-17.

Atia A, Abdullah A. Tocotrienols: Molecular aspect beyond its

antioxidant activity. J Med Res Pract 2013;2(9):246-50.

Packer L, Weber SU, Rimbach G. Molecular aspects of alpha-tocotrienol

antioxidant action and cell signalling. J Nutr 2001;131(2):369S-73.

Shafaat K, Kumar B, Das SK, Ul Hasan R, Prajapati SK. Novel Nano

emulsion as vehicles for transdermal delivery of clozapine in vitro and

in vivo studies. Int J Pharm Pharm Sci 2013;5(3):126-34.

Ali MS, Alam MS, Alam N, Siddqui MR. Preparation, characterization

and stability study of dutasteride loaded Nano emulsion for treatment of

benign prostatic hypertrophy. Iran J Pharm Res 2014;13(4):1125-40.

Honary S, Zahir F. Effect of zeta potential on the properties of

nano drug delivery systems - A review (Part 2). Trop J Pharm Res

;12(2):265-73.

Schuh RS, Bruxel F, Teixeira HF. Physicochemical properties of

Lecithin-based Nano emulsions obtained by spontaneous emulsification

or high-pressure homogenization. Quím Nova 2014;37(7):1193-8.

Molyneux P. The use of stable free radical Diphenylpicrylhydrazyl

(DPPH) for estimating antioxidant activity. J Sci Technol

;26(2):211-9.

Arditiana A, Rochmawati N, Widinugroho P, Puspitasari RD,

Widyaningsih TD. Supplements of black grass jelly and banaba

leaves to treatment cholesterol, hypertension, and diabetes. J Pangan

Agroindustri 2015;3(1):166-73.




About this article

Title

PHYSICAL STABILITY AND ANTIOXIDANT ACTIVITY ASSAY OF A NANOEMULSION GEL FORMULATION CONTAINING TOCOTRIENOL

Keywords

Antioxidant, Stability, Tocotrienol, Nanoemulsion gel.

DOI

10.22159/ijap.2017.v9s1.74_81

Date

30-10-2017

Additional Links

Manuscript Submission

Journal

International Journal of Applied Pharmaceutics
Vol. 9, Special Issue (Oct.), 2017 [PTMDS 2017] Page: 140-143

Online ISSN

0975-7058

Statistics

9 Views | 20 Downloads

Authors & Affiliations

Mentari Mayang Suminar
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia

Mahdi Jufri
Department of Pharmacy, Faculty of Pharmacy, Universitas Indonesia, Depok, Indonesia.
Indonesia


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