• Fadlina Chany Saputri Department of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia
  • Astari Rachma Nityasa Department of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia


Objective: This study aimed to further investigate the antiplatelet and antithrombotic activities of Trigonella foenum-graceum (TFG) in vivo.
Methods: Male mice were divided into two experimental groups (bleeding duration and survival rate). The study groups comprised vehicle controls
(administered carboxymethyl cellulose [CMC]), negative controls (administered CMC), positive controls (administered aspirin), and experimental
treatment groups (administered TFG extract at three doses). Bleeding duration was assessed by excising the tail vein. Survival rate was determined
by inducing thrombosis through intravenous collagen/epinephrine administration.
Results: In mice treated with TFG extract for 7 days, bleeding duration was significantly increased compared with that in controls (p<0.05). Moreover,
mice treated with TFG showed increased survival rates compared with negative controls.
Conclusion: TFG extract showed antithrombotic activity in mice by significantly increasing bleeding duration and survival rate.

Keywords: Antithrombotic, Bleeding duration, Collagen, Epinephrine, Survival rate, Trigonella foenum-graceum.


1. Kim K, Lim KM, Kim CW, Shin HJ, Seo DB, Lee SJ, et al. Black
soybean extract can attenuate thrombosis through inhibition of
collagen-induced platelet activation. J Nutr Biochem 2011;22:964-70.
2. Saputri FC, Nabila N, Mun’im A. Combination of ginger and sappan
wood extract effect on in vivo antithrombotic activity test observation
result of bleeding time. J Young Pharma 2017;9:46-8.
3. Lee JO, Kim C, Lee S, Oak M. Antiplatelet and antithrombotic
activities of Lindera obtusiloba extract in vitro and in vivo. Biomiol
Ther 2010;18:205-10.
4. Parvizpur A, Ahmadiani A, Kamalinejad M. Probable role of spinal
purinoceptors in the analgesic effect of Trigonella foenum (TFG) leaves
extract. J Ethnopharmacol 2006;104:108-12.
5. Kontogiorgis C, Nicolotti O, Mangiatordi GF, Tognolini M, Karalaki F,
Giorgio C, et al. Studies on the antiplatelet and antithrombotic profile
of anti-inflammatory coumarin derivatives. J Enzyme Inhib Med Chem
6. Patil S, Jain G. Holistic approach of Trigonella foenum-graecum in
Phytochemistry and Pharmacology- A Review. CTTS 2014;3:34-48.
7. DiMinno G, Silver MJ. Mouse antithrombotic assay: A simple method
for the evaluation of antithrombotic agents in vivo. Potentiation
of antithrombotic activity by ethyl alcohol. J Pharmacol Exp Ther
8. Rayyan S, Fossen T, Andersen ØM. Flavone C -glycosides from seeds
of fenugreek, Trigonella foenum-graecum L. J Agric Food Chem
9. Ekasari W, Wahyuni TS, Arwaty H, Putri NT. Determination of
effective dose of antimalarial from Cassia spectabilis leaf ethanol
extract in Plasmodium berghei-infected mice. Afr J Infect Dis
2018;121 Suppl:110-5.
10. Kwon SU, Lee HY, Xin M, Ji SJ, Cho HK, Kim DS, et al. Antithrombotic
activity of Vitis labrusca extract on rat platelet aggregation. Blood
Coagul Fibrinolysis 2016;27:141-6.
11. Ku SK, Bae JS. Antiplatelet and antithrombotic activities of
purpurogallin in vitro and in vivo. BMB Rep 2014;47:376-81.
12. Klafke JZ, Arnoldi da Silva M, Fortes Rossato M, Trevisan G, Banderó
Walker CI, Martins Leal CA, et al. Antiplatelet, antithrombotic, and
fibrinolytic activities of Campomanesia xanthocarpa. Evid Based
Complement Alternat Med 2012;2012:954748.
13. Taj Eldin IM, Abdalmutalab MM, Bikir HE. An in vitro anticoagulant
effect of fenugreek (Trigonella foenum-graecum) in blood samples of
normal Sudanese individuals. Sudan J Paediatr 2013;13:52-6.
14. Lee J, Kwon G, Park J, Kim JK, Choe SY, Seo Y, et al. An ethanol
extract of Ramulus Mori improves blood circulation by inhibiting
platelet aggregation. Biosci Biotechnol Biochem 2016;80:1410-5.
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How to Cite
Saputri, F. C., & Nityasa, A. R. (2018). ANTITHROMBOTIC EFFECT OF TRIGONELLA FOENUM-GRACEUM ON COLLAGEN/ EPINEPHRINE-INDUCED THROMBOEMBOLISM IN MICE. International Journal of Applied Pharmaceutics, 10(1), 56-58.
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