HEPATOPROTECTIVE EFFECT OF BELLAMYA JAVANICA: ASPARTATE TRANSAMINASE, ALANINE AMINOTRANSFERASE, AND ALKALINE PHOSPHATASE ACTIVITY, AND LIVER HISTOPATHOLOGY IN MICE INDUCED WITH CARBON TETRACHLORIDE

  • Fadlina Chany Saputri Departmentof Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Carolina Astari Departmentof Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Dyah Adinda Janatry Departmentof Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Azizahwati . Departmentof Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok 16424, Indonesia.
  • Dadang Kusmana Department ofBiology, Faculty of Mathematics and Natural Sciences, Universitas Indonesia, Depok 16424, Indonesia.

Abstract

Objective: The aim of this study was to evaluate the hepatoprotective effects of Bellamya javanica meat (BJM) in mice by quantifying alanine
aminotransferase (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP) activity and observing liver histology.
Methods: A total of 36 white male Sprague-Dawley mice were divided into six groups, namely a normal control group, a negative control group (0.5%
carboxymethyl cellulose), a positive control group (silymarin with a dosage of 9.45 mg/200 g mouse weight), dosage 1 group (BJM powder at 1 g/kg
mouse weight), dosage 2 group (BJM powder at 2 g/kg mouse weight), and dosage 3 group (BJM powder at 4 g/kg mouse weight). Mice were treated
for 14 days. On the 15th day, all groups (except the normal control group) were induced with carbon tetrachloride and fed ad libitum. After 24 h of
induction, ALT, AST, and ALP in serum were quantified, and livers were dissected for histopathological examination.
Results: The results showed that the consumption of BJM at doses of 2 and 4 g/kg mouse weight cast a hepatoprotective effect compared with the
negative control group. In addition, there were significant differences in the hepatoprotective effect between the various doses of BJM. The dosage
with the highest potential hepatoprotective effect was 4 g/kg mouse weight (p<0.05).
Conclusion: B. javanica has potential hepatoprotective effects, with the strongest protection occurring at a 4 g/kg mouse weight dosage.
Hepatoprotection was observed in the form of decreased AST, ALT, and ALP activity and relevant changes in liver histopathology.

Keywords: Alanine aminotransferase, Alkaline phosphatase, Aspartate transaminase, Bellamya javanica, Liver histology.

References

1. Tappi ES, Lintong P, Loho LL. Histopathological features of rat liver of
wistar given tomato juice (Solanum lycopersicum) post-wistar induced
liver injury of carbon tetrachloride (CCl4). J Biomed 2013;1:1126-9.
2. Ali M, Qadir MI, Saleem M, Janbaz KH, Gul H, Hussain L, et al.
Hepatoprotective potential of convolvulus arvensis against paracetamolinduced
hepatotoxicity. Bangladesh J Pharmacol 2013;8:300-4.
3. Zhang ZF, Liu Y, Lu LY, Luo P. Hepatoprotective activity of
Gentiana veitchiorum Hemsl. Against carbon tetrachloride-induced
hepatotoxicity in mice. Chin J Nat Med 2014;12:488-94.
4. Dineshkumar G, Rajakumar R, Mani P, Johnbastin TM. Hepatoprotective
activity of leaves extract of eichhornia crassipes against CCL4 induced
hepatotoxicity albino rats. Int J Pure Appl Zool 2013;1:209-12.
5. Ristiyanti M. Marwoto, RM, Isnaningsih NR. Study on the freshwater
mollusc diversity of the small lakes along Ciliwung and Cisadane
rivers. Berita Biol 2014;13:181-9.
6. Urrego LE, Correa-Metrio A, González C, Castaño AR, Yokoyama Y.
Contrasting responses of two Caribbean mangroves to sea-level
rise in the Guajira Peninsula (Colombian Caribbean). Palaeogeogr
Palaeoclimatol Palaeoecol 2013;370:92-102.
7. Gomes A, Alam MA, Datta P, Bhattacharya S, Gomes A.
Hepatoprotective activity of the edible snail (Bellamia bengalensis)
flesh extract in carbon tetrachloride induced hepatotoxicity in rats.
J Ethnopharmacol 2011;138:228-32.
8. Chakraborty D, Mukherjee M, Maity J. Ethno-medicinal importance of
viviparous gastropod, Bellamya bengalensis (Lamarck, 1822) in terms
of estimation of its proximate protein and amino acid composition. Int
J Recent Sci Res 2015;4:178-80.
9. Wu G, Fang YZ, Yang S, Lupton JR, Turner ND. Glutathione
metabolism and its implications for health. J Nutr 2004;134:489-92.
10. Auliah A. The effect of age on the diversity of amino acid content of
earthworms Lumbricuss rubellus. J Chem 2008;9:37-42.
11. Ranawat L, Bhatt J, Patel J. Hepatoprotective activity of ethanolic
extracts of bark of Zanthoxylum armatum DC in CCl4 induced hepatic
damage in rats. J Ethnopharmacol 2010;127:777-80.
12. Raj B, Singh SJ, Samual VJ, John S, Siddiqua A. Hepatoprotective and
antioxidant activity of Cassytha filiformis against CCl4 induced hepatic
damage in rats. J Pharm Res 2013;7:15-9.
13. Tiwari P, Ahirwae D, Chandy A, Ahirwar B. Evaluation of
hepatoprotective activity of alcoholic and aqueous extracts of
Selaginella lepidophylla. Asian Pac J Trop Dis 2014;4:S81-6.
14. Ramaiah S, Jaeschke H. Role of neutrophils in the pathogenesis of
acute inflammatory liver injury. Toxicol Pathol 2007;35:757-66.
15. Dixit N, Baboota S, Kohli K, Ahmad S, Ali J. Silymarin: A review of
pharmacological aspects and bioavailability enhancement approaches.
Indian J Pharm 2007;39:172-9.
16. Robert SM, James RC, Franklin MR. Hepatotoxicity: Toxic effect on
the liver. In: Williams PL, James RC, Roberts AM, editors. Principles
of Toxicology. New York: John Wiley and Sons; 2000. p. 111-27.
17. Lu CF, Kacew S. Lu’s Basic Toxicology: Fundamentals, Target Organs
and Risk Assessment. 4th ed. London: Taylor and Francais; 2002.
18. Lu M, El-Shazly M, Wu T, Du Y, Chang T, Chen C, et al. Recent
research and development of Antrodia cinnamomea. Pharmacol Ther
2013;139:124-56.
19. Pavarino EC, Russo A, Galbiatti AL, Almeida WP, Bertollo EM.
Glutathione: Biosynthesis and mechanism of action. In: Labrou N,
Flemetakis E, editors. Glutathione: Biochemistry, Mechanisms of
Action and Biotechnological Implications. New York: Nova Science
Publishers; 2013. p. 6-20.
20. Townsend DM, Tew KD, Tapiero H. The importance of glutathione in
human disease. Biomed Pharmacother 2013;57:145-55.
21. Reza HM, Sagor MA, Alam MA. Iron deposition causes oxidative
stress, inflammation, and fibrosis in carbon tetrachloride-induced liver
dysfunction in rats. Bangladesh J Pharmacol 2015;10:152-9.
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Saputri, F. C., Astari, C., Janatry, D. A., ., A., & Kusmana, D. (2018). HEPATOPROTECTIVE EFFECT OF BELLAMYA JAVANICA: ASPARTATE TRANSAMINASE, ALANINE AMINOTRANSFERASE, AND ALKALINE PHOSPHATASE ACTIVITY, AND LIVER HISTOPATHOLOGY IN MICE INDUCED WITH CARBON TETRACHLORIDE. International Journal of Applied Pharmaceutics, 10(1), 203-207. https://doi.org/10.22159/ijap.2018.v10s1.45
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