• Puspita Puspasari Department of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.
  • Fadlina Chany Saputri Department of Pharmacology, Faculty of Pharmacy, Universitas Indonesia, Depok, 16424, Indonesia.


Objective: This study aimed to analyze the effect on wound healing following treatment with a water extract of Mirabilis jalapa L. by investigating
wound contraction and the associated histopathological changes in rat skin.
Methods: Male Sprague-Dawley rats were divided into five groups, namely negative control, positive control (povidone-iodine), dose 1, dose 2, and
dose 3. A 20-×10-mm rectangular wound area was created for the test. In dose 1, 2, and 3 groups, the corresponding dose variation of a 0.5-mL
M. jalapa L. water extract (dose 1: 5% v/v, ≈243.1 mg/kg body weight BW; dose 2: 10% v/v, ≈486.2 mg/kg BW; and dose 3: 20% v/v, ≈972.4 mg/kg BW)
was topically applied for 14 days on open wounds of rats. Widespread wound contractions were measured on days 1, 3, 5, 7, 9, 11, and 13, and
histopathological changes in the skin were observed on day 15 using hematoxylin and eosin staining.
Results: The M. jalapa L. water extract accelerated wound healing. The optimal dose was found to be 20% v/v (≈972.4 mg/kg BW).
Conclusion: M. jalapa L. extracts are potential healing agents for open wounds.

Keywords: Mirabilis jalapa L., Open wound, Povidone-iodine, Histopathology, Wound healing.


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How to Cite
Puspasari, P., & Saputri, F. C. (2018). EFFECT OF THE WATER EXTRACT OF THE FOUR O’CLOCK HERB (MIRABILIS JALAPA L.) ON THE HEALING OF OPEN WOUNDS IN RATS. International Journal of Applied Pharmaceutics, 10(1), 155-158. https://doi.org/10.22159/ijap.2018.v10s1.33
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