EFFECT OF THE WATER EXTRACT OF THE FOUR Oâ€™CLOCK HERB (MIRABILIS JALAPA L.) ON THE HEALING OF OPEN WOUNDS IN RATS
Objective: This study aimed to analyze the effect on wound healing following treatment with a water extract of Mirabilis jalapa L. by investigating
wound contraction and the associated histopathological changes in rat skin.
Methods: Male Sprague-Dawley rats were divided into five groups, namely negative control, positive control (povidone-iodine), dose 1, dose 2, and
dose 3. A 20-Ã—10-mm rectangular wound area was created for the test. In dose 1, 2, and 3 groups, the corresponding dose variation of a 0.5-mL
M. jalapa L. water extract (dose 1: 5% v/v, â‰ˆ243.1 mg/kg body weight BW; dose 2: 10% v/v, â‰ˆ486.2 mg/kg BW; and dose 3: 20% v/v, â‰ˆ972.4 mg/kg BW)
was topically applied for 14 days on open wounds of rats. Widespread wound contractions were measured on days 1, 3, 5, 7, 9, 11, and 13, and
histopathological changes in the skin were observed on day 15 using hematoxylin and eosin staining.
Results: The M. jalapa L. water extract accelerated wound healing. The optimal dose was found to be 20% v/v (â‰ˆ972.4 mg/kg BW).
Conclusion: M. jalapa L. extracts are potential healing agents for open wounds.
Aesculapius Faculty of Medicine, Universitas Indonesia; 2000.
2. Gunawan S. Pharmacology and Therapy. 5th ed. Jakarta: FKUI; 2007.
3. Shaik S, Dhanalakshmi M, Jayasree T. Nephroprotective activity of
leaves of Mirabilis jalapa. L by acetaminophen induced nephrotoxicity.
Int J Pharm Technol 2012;4:4616-29.
4. Kumala S, Tambunan RM, Mochtar D. Anti-bacterial activity test of
ethyl acetate extract at Mirabilis jalapa L. With bioautography method.
J Farmasi Indones 2006;3:78-83.
5. Hajji M, Jarrayab R, Lassoueda I, Masmoudia O, Damakb M, Nasria M.
GC/MS and LC/MS analysis, and antioxidant and antimicrobial
activities of various solvent extracts from Mirabilis jalapa tubers.
Process Biochem 2010;45:1486-93.
6. Zachariah SM, Viswanad V, Aleykutty NA, Jaykar B, Halima OA. Free
radical scavenging and antibacterial activity of Mirabilis jalapa Linn
using in vitro models. Asian J Pharm Clin Res 2012;5:115-9.
7. Rumzhum NN, Rahman MM, Islam MS, Chowdhury SA, Sultana R,
Pervin N. Cytotoxicity and antioxidant activity of extractives from
Mirabilis jalapa. J Pharm Sci 2008;1:85-8.
8. Nath LR, Manjunath KP, Savadi RV, Akki KS. Anti-inflammatory
activity of Mirabilis jalapa Linn. Leaves. J Basic Clin Pharm
9. Puratchikody A, Devi CN, Nagalakhsmi G. Wound healing activity of
Cyperus rotundus Linn. Indian J Pharm Sci 2006;68:97-101.
10. Gogoi J, Nakhuru KS, Policegoudra RS, Chattopadhyay P, Rai AK,
Veer V. Isolation and characterization of bioactive components from
Mirabilis jalapa L. Radix. J Tradit Complement Med 2016;6:41-7.
11. Singh M, Akash G, Mittal SK, Kalia AN. Mirabilis jalapa - A review.
Int J Pharm Med Appl Sci 2012;1:22-43.
12. Govindarajan P, Sarada DV. Isolation and chacterization of stigmasterol
and Î²-sitosterol from Acacia nilotica (l.) Delile ssp Indica (benth.)
Brenan. J Pharm Res 2011;4:3601-2.
13. Arroyo AR, Chacon BL, Maki KA. Screening and selection of plants by
positive pharmacologic effect on jejenum muscular contractility. Pharm
14. Oladunmoye MK. Antioxidant, free radical scavenging capacity
and antimicrobial activities of Mirabilis jalapa. J Med Plants Res
15. Atiyeh BS, Al-Amm CA, Nasser AA. Improved healing of split
thickness skin graft donor sites. J Appl Res 2000;2:78-85.