GARLIC EXTRACT EFFICACY AGAINST THE VIABILITY OF ENTEROCOCCUS FAECALIS (IN VITRO)

  • ANDRIANI OCTAVIA Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • SARWORINI BAGIO BUDIARDJO Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • IKE SITI INDIARTI Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia
  • EVA FAUZIAH Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia
  • MARGARETHA SUHARSINI Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia
  • HERIANDI SUTADI Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia.
  • MOCHAMAD FAHLEVI RIZAL Department of Pediatric Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta 10430, Indonesia

Abstract

Objective: Enterococcus faecalis, Gram-positive facultative anaerobe capable of invading the dentinal tubules, is resistant to several irrigating solutions
and intracanal medications. Motivated by the antibacterial effect exhibited by garlic extract against a wide variety of Gram-positive and Gram-negative
bacteria, as well as anaerobic bacteria such as Lactobacillus and E. faecalis, we aimed in this study to analyze the efficacy of garlic extract against the
viability of E. faecalis from clinical isolates of nonvital primary root canals.
Methods: We used MTT assay to determine the viability of E. faecalis after exposure to increasing concentrations of garlic extract (10%, 25%, 50%,
and 100%) and chlorhexidine (CHX) 2% as a positive control.
Results: We performed the analysis using the Kruskal–Wallis test and the post hoc test for multiple comparisons at a 0.05 significance level. At all
concentrations, garlic extracts decreased the viability of E. faecalis. We found no significant differences between the viability values for 25%, 50%,
and 100% garlic extracts (p>0.05); however, they were significantly different from the 10% extract and CHX 2% (p<0.05), both with a lower viability
values.
Conclusion: Our results showed that garlic extract was effective in decreasing the viability of E. faecalis.

Keywords: Garlic extract, viability, Enterococcus faecalis

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OCTAVIA, A., BUDIARDJO, S. B., INDIARTI, I. S., FAUZIAH, E., SUHARSINI, M., SUTADI, H., & RIZAL, M. F. (2019). GARLIC EXTRACT EFFICACY AGAINST THE VIABILITY OF ENTEROCOCCUS FAECALIS (IN VITRO). International Journal of Applied Pharmaceutics, 11(1), 194-197. https://doi.org/10.22159/ijap.2019.v11s1.17351
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