ISOLATION OF PROTEIN FROM THE SPINE VENOM OF PTEROIS VOLITANS FOUND IN THE INDONESIAN OCEAN, USING A HEATING PROCESS, FOR ANTICANCER, ANTIRETROVIRAL, ANTIBACTERIAL, AND ANTIOXIDANT ASSAYS

Authors

  • FERA IBRAHIM irology and Cancer Pathobiology Research Centre, Faculty of Medicine, Universitas Indonesia, Dr. Cipto Mangunkusumo General Hospital, Jakarta, 10320, Indonesia
  • MUHAMAD SAHLAN Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia, Research Centre for Biomedical Engineering, Faculty of Engineering Universitas Indonesia, Depok, West Java, 16424, Indonesia
  • MIKAEL JANUARDI GINTING Marine Science Postgraduate Program, Faculty of Mathematics and Natural Science, Universitas Indonesia, Depok, West Java, 16424, Indonesia
  • DIAH KARTIKA PRATAMI Laboratory of Pharmacognosy and Phytochemistry, Faculty of Pharmacy, Pancasila University, South Jakarta, 12640, DKI Jakarta, Indonesia
  • HERI HERMANSYAH Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia
  • ANONDHO WIJANARKO Department of Chemical Engineering, Faculty of Engineering, Universitas Indonesia, Depok, West Java, 16424, Indonesia

DOI:

https://doi.org/10.22159/ijap.2021.v13s2.10

Keywords:

Pterois volitans, Crude venom, Anticancer, Antioxidant, Antibacterial, Antiretroviral

Abstract

Objective: This research investigates the antibacterial, anticancer, antioxidant, and antiretroviral activities of the lionfish spine poison extract.

Methods: Isolation and purification of the phospholipase A2 (PLA2) protein obtained from the spine poison were conducted through the following stages, including, extraction of the venom by sonication, heating, and purification using gradual saturation levels of ammonium sulfate. Furthermore, the purity and concentration of PLA2 were analyzed using the Lowry test and Marinetti’s method, respectively, while its protein content was ascertained through SDS-PAGE. Toxicity was then evaluated employing the brine shrimp lethality test (BSLT), and its anticancer activity was assessed in human cervical carcinoma cells (HeLa cells). Finally, its antioxidant, antibacterial, and antiretroviral activities were analyzed using the DPPH method, agar diffusion test against Salmonella sp. and E. coli, and SRV-2 and RT-qPCR tests, respectively.

Results: The protein demonstrated 37.79% inhibition for anticancer activity, IC50 1312 ppm for antioxidant activity, 98.81%, and 89.28% inhibition of E. coli and Salmonella sp. respectively for antibacterial activity and 98.13% inhibition for antiretroviral activity.

Conclusion: It can be concluded that lionfish (Pterois volitans) has the potential to be developed as an antioxidant, anticancer, antibacterial, and antiretroviral agent. Furthermore, the pharmacological activity of its spine venom was determined by isolating PLA2 protein from its extract, using an optimum heating temperature of 70 °C and an ammonium sulfate saturation level of 80%.

Downloads

Download data is not yet available.

References

1. Ritger AL, Fountain CT, Bourne K, Martin Fernandez JA, Pierotti MER. Diet choice in a generalist predator, the invasive lionfish (Pterois volitans/miles). J Exp Mar Bio Ecol 2020;524:151311.
2. Eddy C, Pitt J, Oliveira K, Morris JA, Potts J, Bernal D. The life history characteristics of invasive lionfish (Pterois volitans and P. miles) in Bermuda. Environ Biol Fishes 2019;102:887–900.
3. Albins MA. Invasive pacific lionfish Pterois volitans reduce the abundance and species richness of native bahamian coral-reef fishes. Mar Ecol Prog Ser 2015;522:231–43.
4. Cote IM, Maljkovic A. Predation rates of Indo-pacific lionfish on Bahamian coral reefs. Mar Ecol Prog Ser 2010;404:219–25.
5. Kulbicki M, Beets J, Chabanet P, Cure K, Darling E, Floeter SR, et al. Distributions of Indo-Pacific lionfishes Pterois spp. in their native ranges: implications for the atlantic invasion. Mar Ecol Prog Ser 2012;446:189–205.
6. Vetrano SJ, Lebowitz JB, Marcus S. Lionfish envenomation. J Emerg Med 2002;23:379–82.
7. Church JE, Hodgson WC. The pharmacological activity of fish venoms. Toxicon 2002;40:1083–93.
8. Memar B, Jamili S, Shahbazzadeh D, Bagheri KP. The first report on coagulation and phospholipase A2 activities of persian gulf lionfish, Pterois russelli, an Iranian venomous fish. Toxicon 2016;113:25–31.
9. Nevalainen TJ, Graham GG, Scott KF. Antibacterial actions of secreted phospholipases A2 review. Biochim Biophys Acta (BBA)-Molecular Cell Biol Lipids 2008;1781:1–9.
10. Cipolari OC, de Oliveira Neto XA, Conceicao K. Fish bioactive peptides: a systematic review focused on sting and skin. Aquaculture 2019;515:734598.
11. Savitri IKE, Ibrahim F, Sahlan M, Wijanarko A. Rapid and efficient purification method of phospholipase A2 from Acanthaster planci. Int J Pharm Biosci 2011:2:401–6.
12. Savitri IKE, Sahlan M, Ibrahim F, Wijanarko A. Isolation and characterization of phospholipase A2 from the spines venom of the crown-of-thorns starfish isolated from papua island. Int J Pharma Bio Sci 2012;3:603–8.
13. Sommeng AN, Pratiwi I, Ginting MJ, Sahlan M, Hermansyah H, Wijanarko A. The effects of heating process on protein isolation of lionfish (Pterois volitans) spines venom extract to antioxidant activity assay. In: AIP Conference Proceedings 2019;2193:20007.
14. Dawson JM, Heatlie PL. Lowry method of protein quantification: evidence for photosensitivity. Anal Biochem 1984;140:391–3.
15. Marinetti GV. The action of phospholipase a on lipoproteins. Biochim Biophys Acta Lipids Lipid Metab 1965;98:554–65.
16. Sommeng AN, Sari M, Ginting MJ, Sahlan M, Hermansyah H, Wijanarko A. The influence of heating process on anticancer activity of Pterois volitans (red lionfish) venom extraction against human cervical carcinoma cell. In: AIP Conference Proceedings 2019;2193:03002217.
17. Sommeng AN, Larasati R, Ginting MJ, Pebriani S, Sahlan M, Hermansyah H, et al. Extraction, antioxidant, and bioactive component assay of lionfish venom Pterois volitans. In: AIP Conference Proceedings 2019;2193:030008.
18. Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211–9.
19. Sharma P, Kumar P, Sharma R, Dhot PS. Futuristic scope of stem cells in medicine. Asian J Pharm Clin Res 2016;9 Suppl 1:13–6.
20. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal 2016;6:71–9.
21. Sommeng AN, Tafsili MAB, Ginting MJ, Sahlan M, Hermansyah H, Wijanarko A. Utilization of lionfish (Pterois volitans) venomous spines with effective purification as an alternative antiretroviral HIV/AIDS. AIP Conference Proceedings 2019;2193:30016.
22. Sommeng AN, Arya RMY, Ginting MJ, Pratami DK, Hermansyah H, Sahlan M, et al. Antiretroviral activity of Pterois volitans (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source. Vet World 2019;12:309-15.
23. Sommeng AN, Eka AK, Ginting MJ, Pebriani S, Sahlan M, Hermansyah H, et al. The effect of ammonium sulfate concentration in protein isolation of lionfish (Pterois volitans) spines venom extract for antitumor test. AIP Conference Proceedings 2019;2193:30009.
24. Matulis D. Selective precipitation of proteins. Curr Protoc protein Sci 2016;83:4–5.
25. Raetz CR, Dowhan W. Biosynthesis and function of phospholipids in Escherichia coli. J Biol Chem 1990:265:1235–8.
26. Rogers C, Erkes DA, Nardone A, Aplin AE, Fernandes Alnemri T, Alnemri ES. Gasdermin pores permeabilize mitochondria to augment caspase-3 activation during apoptosis and inflammasome activation. Nat Commun 2019;10:1–17.
27. Dahham SS, Al-Rawi SS, Ibrahim AH, Majid ASA, Majid AMSA. Antioxidant, anticancer, apoptosis properties and chemical composition of black truffle Terfezia claveryi. Saudi J Biol Sci 2018;25:1524–34.
28. Li H, Zhang Z, Li M, Li X, Sun Z. Yield, size, nutritional value, and antioxidant activity of oyster mushrooms grown on perilla stalks. Saudi J Biol Sci 2017;24:347–54.
29. Sudarshan S, Dhananjaya BL. Antibacterial activity of an acidic phospholipase A2 (NN-XIb-PLA 2) from the venom of Naja naja (Indian cobra). Springerplus 2016;5:112.
30. Diniz Sousa R, Caldeira CAS, Kayano AM, Paloschi MV, Pimenta DC, Simoes Silva R, et al. Identification of the molecular determinants of the antibacterial activity of lmut TX, a Lys49 phospholipase A2 homologue isolated from Lachesis muta muta snake venom (Linnaeus, 1766). Basic Clin Pharmacol Toxicol 2018;122:413–23.
31. Sari SP, Isnaini SR, Puspitasari AW. Monitoring side effects of antiretroviral therapy in patients with human immunodeficiency virus/acquired immunodeficiency syndrome. Int J Appl Pharm 2018;10 Special Issue 1:321-4.

Published

10-02-2021

How to Cite

IBRAHIM, F., SAHLAN, M., GINTING, M. J., PRATAMI, D. K., HERMANSYAH, H., & WIJANARKO, A. (2021). ISOLATION OF PROTEIN FROM THE SPINE VENOM OF PTEROIS VOLITANS FOUND IN THE INDONESIAN OCEAN, USING A HEATING PROCESS, FOR ANTICANCER, ANTIRETROVIRAL, ANTIBACTERIAL, AND ANTIOXIDANT ASSAYS. International Journal of Applied Pharmaceutics, 13(2), 53–58. https://doi.org/10.22159/ijap.2021.v13s2.10

Issue

Section

Original Article(s)

Most read articles by the same author(s)

1 2 > >>