EFFECT OF HYDROLYZED RICE FORMULA ON INTESTINAL STRUCTURE OF BALB/C MICE IMMUNIZED WITH COWâ€™S MILK PROTEINS
Objective: In the last few years a new preparation with hydrolyzed rice proteins was introduced and marketed as a useful alternative in the cases of cow's milk allergy.
The objective of our work is to study the consequences of hydrolyzed rice formula on the structure of the intestinal epithelium.
Methods: For the histological study, we have used 4 weeks old 60 female mice Balb/c, weighting (19.50 Â± 0.25) g and distributed as follow: Â
- Â§ Group 1A: 10 mice receiving hydrolyzed rice formula for a period of 28 days after being immunized with native ÃŸ-Lg.
- Â§ Group 1B: 10 mice continue receiving a standard feed for a period of 28 days after being immunized with native ÃŸ-Lg (Positive control).
- Â§ Group 2C: 10 mice receiving hydrolyzed rice formula for a period of 28 days after being immunized with Î±-La protein.
- Â§ Group 2D: 10 mice continue receiving a standard feed for a period of 28 days after being immunized with Î±-La protein (Positive control).
- Â§ Group 3: 20 mice receiving just a standard feed with no treatment (Negative control).
Results: The weight growth of all the experimental groups increases gradually with time, but the consumption of the hydrolyzed rice proteins causes a decrease of the degree of evolution of the physical weight to mice fed with this infantile formula compared with control groups. It also causes an increase of the intestinal villi height at the structure level of the intestinal mucous membrane of mice fed with hydrolyzed rice proteins compared to the positive control groups. The lymphocytic infiltration of mice fed with hydrolyzed rice proteins is similar to the one in negative control group.
Conclusion: The preliminary results show that the hydrolyzed rice formula is a possible alternative to the cowâ€™s milk formula in case of allergy. Further studies are needed to prove its nutritional efficacy.
2. Dupont C, Chouraqui JP, De Boissieu D, et al. Prise en charge diÃ©tÃ©tique de lâ€™allergie aux protÃ©ines du lait de vache. Arch PÃ©diatr 2011;18:79-94.
3. De Boissieu D, Dupont C. Allergie au lait de vache IgE-mÃ©diÃ©e. Arch PÃ©diatr 2006;13:1283-4.
4. Bancroft JD, Gamble M. Theory and practice of histological techniques. New York: Churchill Livingstone; 2002.
5. Bhatia J, Greer F. American academy of Pediatrics committee on nutrition. Use of soy-protein based formulas in infant feeding. J Pediatr 2008;121:1062-8.
6. Nutrition Committee of the French Society of Pediatrics. Phyto-estrogÃ¨nes et aliments Ã base de soja chez le nourrisson et lâ€™enfant: la prudence est de mise. Arch Pediatr 2006;13:1091â€“3.
7. Meister E, Ellnain M, Gieseler H, Aaps. Collapse temperature measurement by freeze-dry microscopy and transferability to freeze drying processes: inluence of solute concentration on collapse behavior and effect on cycle design. Meeting and Exposition Oct 29Nov San Antonio Texas USA 2006;2.
8. Koo W, Lasekan JB. Rice protein-based infant formula: current status and future development. Minerva Pediatr 2007;59:35â€“41.
9. Elbez A. Des Â« laits Â» qui nâ€™en sont pas. Med Enf 2006;26:103-8.
10. Massa G, Vanoppen A, Gillis P, Aerssens P, Alliet P, Raes M. Protein malnutrition due to replacement of milk by rice drink. Eur J Pediatr 2001;160:382-4.
11. Dupont C, Boissieu D. Aliments et dermatite atopique. Rev Fr Allergol 2005;45:114â€“26.
12. Lasekan JB, Koo WK, Walters J. Growth, tolerance and biochemical measures in healthy infants fed a partially hydrolyzed rice protein-based formula: a randomized, blinded, prospective trial. J Am Coll Nutr 2006;25:12-9.
13. Savino F, Castagno E, Monti G, et al. Z-score of weight for age of infants with atopic dermatitis and cowâ€™s milk allergy fed with a rice-hydrolysate formula during the first two years of life. Acta Pediatr 2005;94:115â€“9.
14. Agostoni C, Fiocchi A, Riva E, et al. Growth of infants with IgE-mediated cowâ€™s milk allergy fed different formulas in the complementary feeding period. Pediatr Allergy Immunol 2007;18:599â€“606.
15. Dâ€™Auria E, Sala M, Lodi F. Nutritional value of a rice-hydrolysate formula in infants with cowâ€™s milk protein allergy: a randomized pilot study. J Int Med Res 2003;31:215-22.
16. Agostini C, Axelsson I, Goulet O. Soy protein infant formulae and follow-on formulae: a commentary by the ESPGHAN committee on nutrition. J Pediatr Gastroenterl Nutr 2006;42:352-61.
17. Reche M, Pascual C, Fiandor. The effect of a partially hydrolysed formula based on rice protein in the treatment of infants with cow s milk protein allergy. Pediatr Allergy Immunol 2010;21:577â€“85.
18. Dupont C. Allergies alimentaires: diagnostic, suivi et traitement. Rev Fr Lab 2012;19-22.
19. Addou S, TomÃ© D, Kheroua O, et al. Parenteral immunization to Î²-lactoglobulin modifies the intestinal structure and mucosal electrical parameters in rabbit. Inter Immunophar 2004;4:1559-63.
20. ESPGHAN COMITÃ‰ DE NUTRITION. formules de protÃ©ine de soja pour nourrissons et sur les formules: un commentaire par le ComitÃ© sur la nutrition ESPGHAN. Gastroenterol Nutr J Pediatr 2006;42:352-61.
21. Fiocchi A, Travaini M, Dâ€™Auria E. Tolerance to a rice hydrolysate formula in children allergic to cowâ€™s milk and soy. Clin Exp Allergy 2003;33:1576-80.
22. Fiocchi A, Restani P, Bernardini R. A hydrolyzed rice-based formula is tolerated by children with cowâ€™s milk allergy: a multi-centre study. Clin Exp Allergy 2006;36:311
23. Girardet JP, Rivero M, Orbegozo J, et al. TolÃ©rance dâ€™une formule infantile de protÃ©ines de riz hydrolysÃ©es. Arch PÃ©diatr 2010;17:1-178.