ANTIGENICITY OF HYDROLYZED RICE FORMULA IN ANIMAL MODEL (BALB/C MICE)
Objective: The hydrolyzed formulas of bovine proteins used in the cow's milk allergy treatment contain peptides which can preserve their allergenicity. These last years, a new preparation with hydrolyzed rice proteins was marketed and could establish a useful alternative in the cases of cow's milk allergy.
The objective of our work is to study the biochemical characteristics of an infantile formula based on hydrolyzed rice and its antigenic effect on the Balb/c mice immunized with Î±-La protein.
Methods: In our work, we have realized an electrophoresis on polyacrylamide gel to determine various proteins which compose this infantile formula. Lowry's method is used to determine the amount of proteins in the formula. Our work has also allowed us to study the antigenicity of the hydrolyzed rice formula using ELISA method by the use of Balb/c mice serum.
Results: Our results have shown that
- Â§ The electrophoresis analysis of the hydrolyzed rice formula has shown the absence of protein bands and consequently the lack of intact proteins in the formula.
- Â§ The protein content of the hydrolyzed rice formula is close to the breast milk protein concentration which is adapted to the needs of the infant.
- Â§ The hydrolyzed rice formula reacts very weakly with the anti Î±-La IgG.
Conclusion: The commercial hydrolyzed rice formula can be adapted to cover the needs of the infant. This infantile formula was treated by technological methods to reduce the antigenic potential to prevent Allergy.
2. Dupont C, Chouraqui JP, De Boissieu D, Bocquet A, Bresson JL, Briend A, et al. Prise en charge diÃ©tÃ©tique de lâ€™allergie aux protÃ©ines du lait de vache. Archiv PÃ©diatr 2011;18:79-94.
3. Lowry OH, Rosebrough NJ, Farr AL, Randall RI. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:193-251.
4. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bactriophage T 4. Nat 1970;227:680-5.
5. Catherine J, Eric B. Techniques ELISA en auto-immunitÃ©. GEAI Lâ€™INFO: NumÃ©ro SpÃ©cial; 2005.p. 10-2.
6. Hosking CS, Heine RG, Hill DJ. The Melbourne milk allergy studyâ€“two decades of clinical research. Allergy Clin Immunol Int J World Allergy Org 2000;12:198â€“205.
7. Reche M, Pascual C, Fiandor A, Polanco I, Rivero-Urgell M, Chifre R, et al. The effect of a partially hydrolyzed formula based on rice protein in the treatment of infants with cow's milk protein allergy. Pediatr Allergy Immunol 2010;21:577â€“85.
8. Nilantha L, Yoichi T, Shinjiro T, Hirohide T, Toki T, Masaaki Y. Reducing the antigenicity of milk whey protein using acid proteinases from Monascus pilosus. Pro Biochemistry 2011;46:806â€“10.
9. Girardet JP. Hydrolysats de riz une alternative thÃ©rapeutique intÃ©ressante? RÃ©alit PÃ©diatr 2010;152:cahier1.
10. Turck D. ComitÃ© de Nutrition de la SociÃ©tÃ© FranÃ§aise de PÃ©diatrie. Allaitement maternel: Les bÃ©nÃ©fices pour la santÃ© de lâ€™enfant et de sa mÃ¨re. Arch pÃ©diatr 2005;12:145-65.
11. Vilain AC. Quâ€™est-ce que le lait ? Rev F Allergol 2010;50:124â€“7.
12. Angelika P, Matthias B. Stability of bovine allergens during food processing Annals of Allergy. Asthma and Immunol 2002;89:16â€“20.
13. Frossard P, Tropia L, Hauser C, Eigenmann P. Lymphocytes in Peyer patches regulate clinical tolerance in a murine model of food allergy. J Allergy Clin Immunol 2004;113:958-64.
14. Wal J. Structure and function of milk allergens. Allergy 2001;56:35-8.
15. Giorgio L, Piacentini, Lucia V, Alessandro B, Paola M, Diego G, et al. Allergenicity of a hydrolyzed rice infant formula in a guinea pig model Annals of Allergy. Asthma Immunol 2003;91:161â€“64.
16. Luigi T, Patrizia I, Sergio A, Alexa Z, Alberto M. Use of hydrolysates in the treatment of cow's milk allergy Annals of AllergY. Asthma Immunol 2002; 89:86â€“90.
17. Fiocchi A, Restani P, Bernardini R. A hydrolyzed rice-based formula is tolerated by children with cowâ€™s milk allergy: a multi-centre study. Clin Exp Allergy 2006;36:311â€“6.