COCONUT MILK MODULATE THE ANTIGENICITY OF ALPHA-LACTALBUMIN IN BALB/C MICE


Yamina Benaissa, Samia Addou, Wafaa Dib, Omar Kheroua, Djamel Saidi

Abstract


Objective: The aim of this work was to study the biochemical characteristics of coconut milk and its antigenic effect on the Balb/c mice immunized with α-lactalbumin protein, as well as its consequences on the structure of the intestinal epithelium.

Methods: To achieve the objective of the study, an electrophoresis was realised on a polyacrylamide gel to determine various proteins contained in coconut milk. In addition, Lowry’s method was used to determine the amount of proteins in the formula. The antigenicity of coconut milk in sera was also studied using the Enzyme-Linked Immunosorbent Assay (ELISA) method. For the histological study, 21 w-old mice Balb/c were used and distributed in three groups of 7 mice each. Group 1, received a standard feed with no treatment (Negative control), group 2 and 3 received respectively a standard feed (Positive control) and coconut milk for a period of 28 d after being immunized with α- lactalbumin.

Results: Analysis of the data revealed that the rate of proteins of cow’s milk is higher than that of the coconut milk ( p0.01). However, after carrying out the electrophoresis analysis, the coconut milk showed the absence of intact proteins. The anti α-Lactalbumin IgG titers significantly increased in positive control groups that received coconut milk (p<0.0001). Moreover, there was an increase of the intestinal villi height of mice fed with coconut milk, in the structure level of their intestinal epithelium compared to the negative control group.

Conclusion: The findings of the study provide the evidence that coconut milk is a possible alternative to the cow’s milk formula in case of allergy.


Keywords


Proteins, Coconut milk, Antigenicity, α-Lactalbumin, Balb/c mice, Immunoglobulin G, ELISA, Intestinal epithelium

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References


Host A. Frequency of cow’s milk allergy in childhood. Ann Allergy Asthma Immunol 2002;89:33–7.

Sicherer SH. Epidemiology of food allergy. J Allergy Clin Immunol 2011;127:594–602.

Fiocchi A, Brozek J, Schunemann HJ, Bahna SL, von Berg A, Beyer K. World Allergy organization (WAO) diagnosis and rationale for action against Cow's milk allergy (DRACMA) guidelines. WAO J 2010;3:57-61.

Venter C, Pereira B, Voigt K, Grundy J, Clayton CB, Higgins B. Prevalence and cumulative incidence of food hypersensitivity in the first 3 y* of life. Allergy 2008;63:354-9.

Rona RJ, Keil T, Summers C, Gislason D, Zuidmeer L, Sodergren E. The prevalence of food allergy: a meta-analysis. J Allergy Clin Immunol 2007;120:638-46.

Hegde BM. Coconut oil-Ideal fat next only to Mother’s Milk. J Indian Acad Clin Med 2006;7:16-9.

DebMandal M, Mandal S. Coconut (Cocosnucifera L.: Arecaceae): in health promotion and disease prevention. Asian Pac J Trop Med 2011;4:241-7.

Effiog GS, Ebong PE, Eyong EU, Uwah AJ, Ekong UEO. Amelioration of chloramphenicol induced toxicity in rats by coconut water. J Appl Sci Res 2010;6:331-5.

Wattanapahu S, Suwonsichon T, Jirapakkul W, Kasermsumran S. Categorization of coconut milk products by their sensory characteristics. Kasetsart J Nat Sci 2012;46:944-54.

Lowry OH, Rosebrough NJ, Farr Al, Randall RI. Protein measurement with the Folin phenol reagent. J Biol Chem 1951;193:193-251.

Leammli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nat 1970;227:680-5.

Dib W, Chekroun A, El-Ghaish S, Choiset Y, Chobert JM, Haertlé T, et al. Effects of orally administered Enterococcus faecium on the immune systemic response and intestinal epithelial structure in balb/c mice immunized by bovine β-lactoglobulin. Int J Pharm Pharm Sci 2014;6:587-90.

Johanet C, Ballot E. Techniques ELISA en auto-immunité. GEAI L’INFO: Numéro Spécial 2005. p. 10-2.

Garcia RN, Arocena RV, Laurena AC, Tecson-Mendoza EM. 11S and 7S globulins of coconut (Cocos nucifera L.): purification and characterization. J Agric Food Chem 2005;53:1734-9.

Saïdi D, Heyman M, Kheroua O, Boudraa G, Bylsma P, Kerroucha R, et al. Response to beta-lactoglobulin in infants with cow's milk allergy. CR Acad Sci III 1995;318:683-90.

Frossard P, Trropia L, Hauser C, Eigenmann P. Lymphocytes in peyer patches regulate clinical tolerance in a murine model of food allergy. J Allergy Clin Immunol 2004;113:958-64.

Dutau G, Dutau H, Rancé F, Juchet A, Rittie JL, Bremont F, et al. Allergie IgE-dependante à la noix de coco: a propos d’un cas avec syndrome oral et manifestations Cutanées=IgE-mediated allergy to coconut. La Semaine des Hôpitaux De Paris 1998;74:650-2.

Teuber SS, Peterson WR. Systemic allergic reaction to coconut (cocos nucifera) in 2 subjectswith hypersensitivity to tree nut and demonstration of cross-reactivity to legumin-like seed strorage proteins: new coconut and walnut food allergens. J Allergy Clin Immunol 1999;103:1180-5.

Dupont C. Allergies alimentaires: diagnostic, suivi et traitement. Rev Française Des Laboratoires 2012;42:19-22.

Addou S, Tome D, Kheroua O, Saidi D. Parenteral immunization to β-lactoglobulin modifies the intestinal structure and mucosal electrical parameters in rabbit. Int Immunopharmacol 2004;4: 1559-63.

Fiocchi A, Restani P, Bernardini R. A hydrolyzed rice-based formula is tolerated by children with cow’s milk allergy: amulti-centre study. Clin Exp Allergy 2006;36:311.

Fiocchi A, Travaini M, D’Auria E. Tolerance to a rice-hydrolysate formula in children allergic to cow’s milk and soy. Clin Exp Allergy 2003;33:1576-80.




About this article

Title

COCONUT MILK MODULATE THE ANTIGENICITY OF ALPHA-LACTALBUMIN IN BALB/C MICE

Keywords

Proteins, Coconut milk, Antigenicity, α-Lactalbumin, Balb/c mice, Immunoglobulin G, ELISA, Intestinal epithelium

DOI

10.22159/ijpps.2017v9i3.15753

Date

03-02-2017

Additional Links

Manuscript Submission

Journal

International Journal of Pharmacy and Pharmaceutical Sciences
Vol 9, Issue 3, 2017 Page: 290-293

Online ISSN

0975-1491

Statistics

129 Views | 89 Downloads

Authors & Affiliations

Yamina Benaissa
Laboratory of Physiology of the Nutrition and Food Safety, Department of Biology, Faculty of Science, University of Oran 1 Ahmed Ben Bella, 31000 Oran, Algeria
Algeria

Samia Addou
Laboratory of Physiology of the Nutrition and Food Safety, Department of Biology, Faculty of Science, University of Oran 1 Ahmed Ben Bella, 31000 Oran, Algeria

Wafaa Dib
Laboratory of Physiology of the Nutrition and Food Safety, Department of Biology, Faculty of Science, University of Oran 1 Ahmed Ben Bella, 31000 Oran, Algeria

Omar Kheroua
Laboratory of Physiology of the Nutrition and Food Safety, Department of Biology, Faculty of Science, University of Oran 1 Ahmed Ben Bella, 31000 Oran, Algeria

Djamel Saidi
Laboratory of Physiology of the Nutrition and Food Safety, Department of Biology, Faculty of Science, University of Oran 1 Ahmed Ben Bella, 31000 Oran, Algeria


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