ANTIDIABETIC STUDY OF COMBINATION OF ANDROGRAPHIS PANICULATA (BURM. F.) WALLICH. EX NEES. HERBS EXTRACT AND GUAZUMA ULMIFOLIA LAMK. LEAVES EXTRACT IN OBESE DIABETIC MICE MODEL

  • Widhya Aligita Bandung School of Pharmacy, Bandung, Indonesia
  • Neng Fisheri Kurniati Bandung Institute of Technology
  • Elin Yulinah Sukandar Bandung Institute of Technology

Abstract

Objective: This research was to investigate the activity of Andrographis paniculata extract (APE) and Guazuma ulmifolia extract (GUE) in treating obesity integrated diabetic conditions.

Methods: There were two stages of inductions: the first stage was a 42 d of high carbohydrate intake (obese with insulin resistance mice model), followed by second stage induction by giving of alloxan 50 mg/kg bw intravenously (obese diabetic mice model). Animals with glucose level>200 mg/dl were then treated with metformin 195 mg/kg bw, glibenclamide 0.65 mg/kg bw, APE 2 g/kg bw, GUE 0.5 g/kg bw, combination of APE 1 g/kg bw and GUE 0.25 g/kg bw, or combination of APE 2 g/kg bw and GUE 0.5 g/kg bw for 14 d.

Results: The results showed that APE alone, GUE alone, and the combination of APE 2 g/kg bw and GUE 0.5 g/kg bw could improve insulin sensitivity in obese with insulin resistance mice model. Whereas the combination of APE 2 g/kg bw and GUE 0.5 g/kg bw could significantly decrease blood glucose level and body weight (p<0.05) in obese diabetic mice model compare to APE alone, GUE alone, or a combination of APE 1 g/kg bw and GUE 0.25 g/kg bw.

Conclusion: It is concluded that APE 2 g/kg bw, GUE 0.5 g/kg bw, and combination of APE 2 g/kg bw and GUE 0.5 g/kg bw can improve insulin resistance conditions caused by obesity, while combination of APE 2 g/kg bw and GUE 0.5 g/kg bw has the best activity in treating obese diabetic conditions.

 

Keywords: Diabetes mellitus, Obesity, Andrographis paniculata, Guazuma ulmifolia, Mice

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References

1. American Diabetes Association. Diagnosis and classification of diabetes mellitus. Diabetes Care 2007;30 Suppl 1:42-7.
2. Riaz S. Diabetes mellitus. Sci Res Essays 2009;4:367-73.
3. Thevenod F. Pathophysiology of diabetes mellitus type 2: roles of obesity, insulin resistance, and β-cell dysfunction. Diabetes Cancer Epidemiol Evidence Molecular Links 2008;9:1-18.
4. Poitout V, Robertson RP. Minireview: secondary–cell failure in type 2 diabetes—a convergence of glucotoxicity and lipotoxicity. Endocrinol 2002;143:339-42.
5. Tattarani PA. Pathophysiology of obesity-induced insulin resistance and type 2 diabetes mellitus. Eur Rev Med Pharmacol Sci 2002;6:27-32.
6. Yaturu S. Obesity and type 2 diabetes. J Diabetes Mellitus 2011;1:79-95.
7. Backer CA, Bakhuizen van den Brink Jr RC. Flora of Java Volume II. the Netherlands: N. V. P. Noordhoff–Groningen; 1965. p. 574.
8. Departemen Kesehatan RI. Inventaris tanaman obat indonesia (I) Jilid 1. Jakarta: departemen kesehatan RI 2000. p. 29-30, 125-6.
9. Chowdhury A, Biswas SK. Comparative study of hypoglycemic effect of ethanolic and hot water extracts of Andrographis paniculata in alloxan induced rat. Int J Pharm Sci Res 2012;3:815-7.
10. Hossain Md A, Roy BK, Ahmed K, Chowdhury AMS, Rashid MA. Antidiabetic activity of Andrographis paniculata. Dhaka Univ J Pharm Sci 2007;6:15-20.
11. Jayakumar T, Hsieh CY, Lee JJ, Sheu JR. Review article: experimental and clinical pharmacology of Andrographis paniculata and Its major bioactive phytoconstituent andrographolide. J Evidence-Based Complementary Altern Med 2013. doi.org/10.1155/2013/846740. [Article in Press]
12. Jarukamjorn K, Nemoto N. Pharmacological aspects of Andrographis paniculata on health and its major diterpenoid constituent andrographolide. J Health Sci 2008;54:370-81.
13. Chao WW, Lin BF. Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian). Chin Med 2010;5:17.
14. Yulinah E, Sukrasno, Fitri MA. Aktivitas antidiabetika ekstrak etanol herba sambiloto (Andrographis paniculata Nees (Acanthaceae)). J Med Sci 2001;6:13-20.
15. Subramanian R, Asmawi MZ, Sadikun A. Effect of ethanolic extract of Andrographis paniculata (Burm. f.) nees on a combination of fat–fed diet and low dose streptozotocin-induced chronic insulin resistance in rats. Diabetologia Croatia 2008;37:13-22.
16. Backer CA. Bakhuizen van den Brink Jr. RC. Flora of Java. Vol. I. The Netherlands: N. V. P. Noordhoff–Groningen; 1963. p. 408.
17. Departemen Kesehatan RI. Farmakope Herbal Indonesia Edisi 1. Jakarta: Departemen Kesehatan RI; 2008. p. 36-40, 122-7.
18. Sukandar YE, Elfahmi, Nurdewi. Pengaruh pemberian ekstrak air daun jati belanda (Guazuma Ulmifolia Lamk.) terhadap kadar lipid darah pada tikus jantan. J Korean Med Sci 2009;8:102-12.
19. Adnyana IK, Yulinah E, Yuliet, Kurniati NF. Antidiabetic activity of aqueous leaf extract of Guazuma ulmifolia Lamk., ethanolic extract of Curcuma xanthorriza and their combinations in alloxan-induced diabetic mice. World Res J Med Aromat Plants 2013;7:158-64.
20. Lian JH, Xiang YQ, Guo L, Hu WR, Gong BQ. The use of high-fat/carbohydrate diet-fed and streptozotocin-treated mice as a suitable animal model of type 2 diabetes mellitus, Scand. J Lab Anim Sci 2007;27:21-9.
21. Setiawan F, Adnyana IK, Sukandar EY, Sukrasno, Pebritinasari R. animal model of obesity in populations with high carbohydrate diet. Obes Rev 2014;15: T6-S28.53.
22. Srinivasan K, Ramarao P. Animal models in type 2 diabetes research: an overview. Indian J Med Res 2007;125:451-72.
23. Fitrianingsih SP, Purwanti L. Uji efek hipoglikemik ekstrak air kulit buah pisang ambon putih [Musa (AAA Group)] terhadap mencit model hiperglikemik galur swiss webster. Prosiding SNaPP 2012;3:73-80.
24. Anfaliah S, Soemardji AA, Fidrianny I. Kajian aktivitas antidiabetes fraksi air herba kemangi (Ocimum americanum L.) pada mencit swiss webster jantan. Sekolah Farmasi ITB; 2007.
25. Ble-Castillo JL, Aparicio-Trapala AA, Juárez-Rojop IE, Torres-Lopez JE, Mendez JD, Aguilar-Mariscal H, et al. Differential effects of high-carbohydrate and high-fat diet composition on metabolic control and insulin resistance in normal rats. Int J Environ Res Public Health 2012;9:1663-76.
26. Dipiro JT, Talbert RL, Yee GC, Matzke GR, Wells BG, Posey LM. Pharmacotherapy–a pathophysiologic approach. 7th ed. New York: McGraw Hill Medical; 2008. p. 1220-7.
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Aligita, W., N. F. Kurniati, and E. Y. Sukandar. “ANTIDIABETIC STUDY OF COMBINATION OF ANDROGRAPHIS PANICULATA (BURM. F.) WALLICH. EX NEES. HERBS EXTRACT AND GUAZUMA ULMIFOLIA LAMK. LEAVES EXTRACT IN OBESE DIABETIC MICE MODEL”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 1, Nov. 2015, pp. 316-20, https://innovareacademics.in/journals/index.php/ijpps/article/view/9409.
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