• Sri Wahyuningsih School of Pharmacy, Bandung Institute of Tecnology, Jalan Ganeca no. 10 Bandung,Indonesia
  • Elin Yulinah Sukandar
  • Sukrasno .
  • Debby Nadya Lofika


Objective: The objective of this study was to determine anti-hyperuricemia activity of ethanol extract of roselle calyx (Hibiscus sabdariffa L) and its fraction on male Wistar rats.

Methods: Two different methods were used. First method induction was done by giving potassium oxonate and diet high purine whereas allopurinol as a comparison. The parameter was level uric acid in serum (uricostatic activity). Second methods induction was done by giving potassium oxonate intraperitoneally and sodium urate orally; with pprobenecid as comparison Parameters used were uric acid level in serum and urine (uricosuric activity).

Results: Ethanol extract roselle calyx at a dose of 40 mg/kg bw, water fraction at a dose of 37.5 mg/kg bw and ethyl acetate fraction at a dose of 6.25 mg/kg bw at day 14 showed a significant reduction in serum uric acid level compared to control group (p<0.05). Besides that ethanol extract of Roselle calyx at dose 80 mg/kg bw, water fraction at doses 37.5 and 75 mg/kg bw also ethyl acetate at doses 6.25 and 12.5 mg/kg bw had uricosuric effect that increased the excretion of uric acid in urine significantly compared to control group (p<0.05).

Conclusion: The water fraction of roselle calyx at doses 37.5 and 75 mg/kg bw and ethyl acetate at dose 6.25 and 12.5 mg/kg bw was effective as an anti-hyperuricemic agent. The ethanol extract of rosele calyx and its fraction had a uricosuric effect similar to probenecid. The best uricosuric effect was showed by ethyl acetate fraction at dose 6.25 mg/kg bw.

Keywords: Hibiscus sabdariffa Linn, Water fraction, Ethyl acetate fraction, Calyx, Uric acid, Antihyperuricemia


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How to Cite
Wahyuningsih, S., E. Y. Sukandar, S. ., and D. N. Lofika. “ANTIHYPERURICEMIA ACTIVITY OF THE ETHANOL EXTRACT OF ROSELLE CALYX AND ITS FRACTION (HIBISCUS SABDARIFFA LINN) ON MALE WISTAR RATS”. International Journal of Pharmacy and Pharmaceutical Sciences, Vol. 8, no. 3, Mar. 2016, pp. 278-80,
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