ANTIOXIDANT ACTIVITIES OF ARABICA GREEN COFFEE FROM THREE REGIONS USING ABTS AND DPPH ASSAYS
Objectives: The aim of this research were to determine antioxidant activity from various extracts of arabica green coffee from three different regions
using two methods of antioxidant testing which were 2,2â€™-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl
(DPPH) and correlation of total phenolic, flavonoid, and carotenoid content in various extracts of arabica coffee with their IC50 of ABTS and IC50 of
DPPH antioxidant activities.
Methods: Extraction was conducted by reflux using various polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant
activities using ABTS and DPPH assays, determination of total phenolic, flavonoid, and carotenoid content were performed by UV-visible
spectrophotometry and its correlation with IC50 of ABTS and IC50 of DPPH scavenging activities were analyzed by Pearsonâ€™s method.
Results: The lowest IC50 of ABTS scavenging activity 3.47 Âµg/ml was given by n-hexane extract of Toraja arabica coffee, while ethanolic extract of
Lintong arabica coffee gave the lowest IC50 of DPPH scavenging activity 0.7 Âµg/ml. Ethanolic extract of Madailing arabica coffee gave the highest
phenolic content, and its ethyl acetate extract had the highest total flavonoid. There were negative and high correlation between phenolic content and
carotenoid content in Lintong arabica coffee extract with their IC50 of DPPH.
Conclusions: All of the arabica green coffee extracts from three regions except LIN1 by ABTS method were categorized as a very strong antioxidant and
except TOR1 for DPPH method. Phenolic and carotenoid compounds in Lintong arabica coffee extracts were the major contributor in IC50 of DPPH
scavenging activities. Arabica coffee extracts from Lintong showed the linear result in ABTS and DPPH assays.
Keywords: Antioxidant, 2,2â€™-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid), 2,2-diphenyl-1-picrylhydrazyl, Arabica green coffee, Phenolic,
2. Carloni P, Tiano L, Padella L, Bacchetti T, Customu C, Kay A, Damiani E. Antioxidant activity of white, green and black tea obtained from the same tea cultivar. Food Res Int 2013; 53: 900â€“8.
3. Xu BJ, Chang SK. Total phenolic content and antioxidant properties of eclipse black beans (Phaseolus vulgaris L.) as affected by processing methods. J Food Sci 2008; 73(2): H19-27.
4. Pellegrini N, Serafini M, Colombi B, Rio DD, Salvatore S. Total antioxidant capacity of plant food, beverages and oils consumed in Italy assessed by three different in vitro assays. J Am Coll Nutr 2003; 133: 2812-9.
5. Fidrianny I, Windyaswari AS, Wirasutisna KR. Antioxidant capacities of various leaves extract from five colors varieties of sweet potatoes tubers using ABTS, DPPH assays and correlation with total flavonoid, phenolic, carotenoid content. Res J Med Plant. 2013; 7(3): 130-40.
6. Khokhar S, Magnusdottir SG. Total phenol, catechin, and caffeine contents of teas commonly consumed in the United kingdom. J Agric Food Chem 2002; 50(3): 565-70.
7. Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid content of some selected Iranian medicinal plants. Afr J Biotechnol 2006; 5(11): 1142-5.
8. Ramalakshmi K, Kubra IR, Rao LJM. Antioxidant potential of low-grade coffee beans. Food Res Int 2008; 41: 96â€“103.
9. Fidrianny I, Permatasari L, Wirasutisna KR, Antioxidant activities from various bulbs extracts, of three kinds allium using DPPH, ABTS assays and correlation with total phenolic, flavonoid, carotenoid content. Int. J. Res. Pharmaceutical Sci 2013; 4(3): 438-44.
10. Win M, Hamid A, Baharin B, Anwar FS, Pak-Dek MS. Phenolic compounds and antioxidant activity of peanut's skin, hull, raw kernel and roasted kernel flour. Pak J Bot 2011; 43(3): 1635-42.
11. Mashkor IMAAL. Phenolic content and antioxidant activity of fenugreek seeds extract. Int J Pharmacogn Phytochem Res 2014; 6(4): 841-4.
12. Thaipong K, Boonprakob U, Crosby K, Zevallos LC, Byrne DH. Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts. J Food Comp Anal 2006; 19: 669-75.
13. Parras P, MartÄ±Â´nez-Tome M, JimeÂ´nez AM, Murcia MA. Antioxidant capacity of coffees of several origins brewed following three different procedures. Food Chem 2007; 102: 582â€“92.
14. Brezova V, Å lebodova A, StaÅ¡ko A. Coffee as a source of antioxidants: An EPR study. Food Chem 2009; 114: 859â€“68.
15. Li XC, Wang XZ, Chen DF, Chen SZ. Antioxidant activity and mechanism of protochatechuic acid in vitro. J Funct Food Health Dis 2011; 1: 232-44.
16. Bedawey AA. Characteristics of antioxidant isolated from some plants sources, Shibin El-Kom: Cairo; 2010. p. 1-11.
17. Blois MS. Antioxidant determination by the use of stable free radicals. Nature 1958; 181: 1199-2000.
18. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in propolis by two complementary colorimetric methods. J Food Drug Anal 2002; 10: 178-82.
19. Nicoli MC, Anese M, Manzocco L, Lerici CR. Antioxidant properties of coffee brews in relation to the roasting degree. Lebensm Wiss u Technol 1997; 30: 292â€“97.
20. Castillo MDD, Ames JM, Gordon MH. Effect of roasting on the antioxidant activity of coffee brews. J Agric Food Chem 2002; 50: 3698-703.
21. Naidu MM, Sulochanamma G, Sampathu SR, Srinivas P. Studies on extraction and antioxidant potential of green coffee. Food Chem 2008; 107: 377â€“84.
22. Richelle M, Tavazzi I, Offord E. Comparison of the antioxidant activity of commonly consumed polyphenolic beverages (coffee, cocoa, and tea) prepared per cup serving. J Agric Food Chem 2001; 49: 3438-42.
23. Heim KE, Tagliaferro AR, Bobilya DJ. Flavonoid antioxidants: Chemistry, metabolism and structure-activity relationships. J Nutr Biochem 2002; 13: 572 -84.
24. Foote, CS. Free radicals in biology. 3rd ed. New York: Academic Press; 1976
25. Beutner S, Bloedorn B, Hoffmann T, Martin HD. Synthetic singlet oxygen quenchers. Methods Enzymol 2000; 319: 226-41.
26. Charles DJ. Antioxidant properties of spices shells and other. London: John Willey; 2013.
27. Kobayashi M, Sakamoto Y. Singlet oxygen quenching ability of astaxanthin esters from the green alga Haematococcus pluvialis. Biotech Lett 1999; 21: 265-9.
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