ANTI-INFLAMMATORY STUDY OF ANREDERA CORDIFOLIA LEAVES AND CENTELLA ASIATICA HERBS AND ITS COMBINATIONS USING HUMAN RED BLOOD CELL-MEMBRANE STABILIZATION METHOD

  • Entris Sutrisno Bandung School of Pharmacy
  • Ketut Adnyana I
  • Elin Yulinah Sukandar
  • Irda Fidrianny
  • Widhya Aligita

Abstract

Objective: Inflammation is body reactions in response to tissue injury and infection. In 2011, non-steroidal anti-inflammatory drug (NSAID) was the
highest demand drug in Indonesia. However, long-term treatment using NSAID can cause several side effects to cardiovascular and digestive system.
This research aimed to investigate anti-inflammatory properties of binahong leaves (Anredera cordifolia) and pegagan herbs (Centella asiatica).

Methods: Ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs was evaluated for its anti-inflammatory properties using human red
blood cell (RBC) – membrane stabilization assay. The extract concentrations used in this study was 100, 200, 400, and 800-ppm, and apigenin and
asiaticoside concentration were 1, 2, 3, 4, 5, 6, 10, and 100 ppm. Diclofenac natrium (DN) was used as a standard drug.
Results: The results showed that A. cordifolia extract (ACE) alone, C. asiatica extract (CAE) alone, and the combination of ACE and CAE could inhibit the
hemolysis of RBC in hypotonic solution. The optimum concentration for ACE alone was 100 ppm; for CAE alone was 400 ppm; and for the combination
of ACE and CAE was 50 ppm and 50 ppm, respectively. Apigenin and asiaticoside in concentration of 1-10 ppm showed more than 97% inhibition of
hemolysis. DN as a standard drug showed optimum inhibition at concentration of 400 ppm.
Conclusion: The ethyl alcohol extract of A. cordifolia leaves and C. asiatica herbs showed anti-inflammatory activity, both as a single treatment or as
combinations, and apigenin and asiaticoside were responsible for anti-inflammatory activity in C. asiatica.
Keywords: Anti-inflammation, Human red blood cell – membrane stabilization, Anredera cordifolia, Centella asiatica.

References

1. Joyce KL, Hayes ER. Farmakologi Pendekatan Proses Keperawatan. Jakarta: Penerbit Buku Kedokteran ECG; 1996.
2. McGettigan P, Henry D. Use of non-steroidal anti-inflammatory drugs that elevate cardiovascular risk: An examination of sales and essential medicines lists in low-, middle-, and high-income countries. PLoS Med 2013;10(2):e1001388.
3. Badan Pengawas Obat dan Makanan Republik Indonesia (BPOM RI). Taksonomi Koleksi Tanaman Obat Kebun Tanaman Obat Citeureup. Vol 1. Jakarta: BPOM RI Direktorat Obat Asli Indonesia; 2008.
4. Yuziani, Harahap U, Karsono. Evaluation of analgesic activities of ethanolic extract of Anredera cordifolia (Ten) steenis leaf. Int J PharmTech Res 2014;6(5):1608-10.
5. Wahjuni S. Anti-hypercholesterolemia of Anredera cordifolia in hypercholesterolemia rat wistar through decrease of malondialdehyde and 8-hydroxy-diguanosine. Indones J Biomed Sci 2014;8(1):4-7.
6. Sukandar EY, Fidrianny I, Adiwibowo IF. Efficacy of ethanol extract of Anredera cordifolia (Ten) steen is leaves on improving kidney failure in rats. Int J Pharmacol 2011;7:850-5.
7. Yuliani SH, Fudholi A, Pramono S, Marchaban. The effect of formula to physical properties of wound healing gel of ethanolic extract of binahong (Anredera cordifolia (Ten) Steenis). Int J Pharm Sci Res
2012;3(72):4254-9.
8. Kasahara YS. Medicinal Herbs Index in Indonesia. Jakarta: PT Eisai Indonesia; 1986.
9. Dalimartha S. Atlas Tumbuhan Obat Indonesia Jilid 2. Jakarta: Trubus Agriwidaya; 2000.
10. Seevaratnam V, Banumathi P, Premalatha MR, Sundaram SP, Arumugam T. Functional properties of Centella asiatica (L.): A review. Int J Pharm Pharm Sci 2012;4 suppl 5:8-14.
11. Hashim, P. Mini review: Centella asiatica in food and beverage applications and its potential antioxidant and neuroprotective effect. Int Food Res J 2011;18(4):1215-22.
12. George M, Joseph L, Ramaswamy. Anti-allergic, anti-pruritic, and anti-inflammatory activities of Centella asiatica extracts. Afr J Tradit Complement Altern Med 2009;6(4):554-9.
13. Chowdhury A, Azam S, Jainul MA, Faruq KO, Islam A. Antibacterial activities and in vitro anti-inflammatory (membrane stability) properties of methanolic extracts of Gardenia coronaria leaves. Int J Microbiol 2014. doi: org/10.1155/2014/410935.
14. Gadamsetty G, Maru S, Sarada NC. Antioxidant and anti-inflammatory activities of the methanolic leaf extract of traditionally used medicinal plant Mimusops elengi L. J Pharm Sci Res 2013;5(6):125-30.
15. Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-inflammatory activity of methanol extract of garden egg (Solanum aethiopicum). Daru 2012;20(1):76.
16. Rajalakshmi GR, Harindran J. Anti-inflammatory activity of Wrightia tinctoria leaves by membrane stabilization. Int J Pharm Sci Res 2012;3:497-9.
17. Chippada SC, Volluri SS, Bammidi SR, Valangapati M. In vitro antiinflammatory activity of methanolic extract of Centella asiatica by HRBS membrane stabilisation. Rasayan J Chem 2011;4(2):457-60.
18. Saha S, Guria T, Singha T, Maity TK. Evaluation of analgesic and anti-inflammatory activity of chloroform and methanol extracts of Centella asiatica linn. ISRN Pharmacol 2013;2013:789613.
19. Moura-Letts G, Villegas LF, Marçalo A, Vaisberg AJ, Hammond GB. In vivo wound-healing activity of oleanolic acid derived from the acid hydrolysis of Anredera diffusa. J Nat Prod 2006;69(6):978-9.
Statistics
478 Views | 413 Downloads
Citatons
How to Cite
Sutrisno, E., K. Adnyana I, E. Yulinah Sukandar, I. Fidrianny, and W. Aligita. “ANTI-INFLAMMATORY STUDY OF ANREDERA CORDIFOLIA LEAVES AND CENTELLA ASIATICA HERBS AND ITS COMBINATIONS USING HUMAN RED BLOOD CELL-MEMBRANE STABILIZATION METHOD”. Asian Journal of Pharmaceutical and Clinical Research, Vol. 9, no. 5, Sept. 2016, pp. 78-80, doi:10.22159/ajpcr.2016.v9i5.11973.
Section
Original Article(s)

Most read articles by the same author(s)

1 2 > >>