THE CAPABILITY OF BRINE SHRIMP TEST AS A TERATOGENICITY SCREENING SYSTEM


Meti Widiya Lestari, Andreanus A Soemardji, Irda Fidrianny, Ayda T Yusuf

Abstract


ABSTRACT
Objectives: The goals of this study were to analyze the capability of brine shrimp test (BST) as a potent teratogenicity screening system on teratogenic
agents (methotrexate, captopril, diclofenac, phenytoin, warfarin, and valproic acid).
Methods: Artemia cysts were hatched into 1st stage nauplii, then taken and put into seawater medium which contain test substance and kept alive until
2nd stage, 3rd stage, and 4th stage, and number of deaths, morphological abnormalities, body length, and retarded of development were observed for each stage.
Results: Hatch ability of cysts in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml, diclofenac 0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid
2.5 mg/ml were significantly different compared to control (p<0.05). Nauplii survival in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml, diclofenac
0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid 2.5 mg/ml were significantly different to control (p<0.05). The morphological abnormalities
was found in methotrexate 0.015 mg/ml, captopril 0.25 mg/ml. Nauplii with retarded development were expressed in methotrexate 0.015 mg/ml,
captopril 0.25 mg/ml, diclofenac 0.075 mg/ml, phenytoin 1.56 mg/ml, and valproic acid 2.5 mg/ml. Significant difference in body length was presented
in captopril 0.25 mg/ml, and phenytoin 1.56 mg/ml compared to control (p<0.05).
Conclusion: BST can be used as an alternative method of the teratogenic screening test, although not as sensitive teratogenic tests on mammals. This
screening method was not suitable for a compound which its chemical characteristic can change the tonicity of the medium.
Keywords: Brine shrimp test, Teratogenicity, Methotrexate, Captopril, Diclofenac, Phenytoin, Warfarin, Valproic acid.


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References


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About this article

Title

THE CAPABILITY OF BRINE SHRIMP TEST AS A TERATOGENICITY SCREENING SYSTEM

DOI

10.22159/ajpcr.2017.v10i3.16511

Date

01-03-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 3 March 2017 Page: 454-457

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

83 Views | 91 Downloads

Authors & Affiliations

Meti Widiya Lestari
Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Bandung Institute of Technology, Indonesia
Indonesia

Andreanus A Soemardji

Irda Fidrianny

Ayda T Yusuf


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