BRINE SHRIMP TERATOGENIC SCREENING TEST ON SOME SELECTED PLANTS


Meti Widiya Lestari, Irda Fidrianny, Ayda T Yusuf, Andreanus A Soemardji

Abstract


 

 Objectives: The aims of this research were to analyze teratogenic properties on some selected plants which often used for woman’s health were pomegranate (Punica granatum L.) pericarp, coriander (Coriandrum sativum L.) seeds, liman (Elephantopus scaber L.) leaves, fennel (Foeniculum vulgare M.) seeds, and kaempferia (Kaempferia galanga L.) rhizomes using brine shrimp teratogenic screening test (BSTST) method.

Methods: Artemia cysts were hatched into first stage nauplii then taken and put into seawater medium which contain test substance and kept alive until the second stage, third stage, and fourth stage and then observed number of deaths, morphological abnormalities, body length, and retarded of development for each stage.

Results: Hatchability of cysts in fennel seeds compared extracts 1 and 0.5 mg/ml, coriander seeds extract 1 mg/ml, pomegranate rinds extract 6 mg/ml, and liman leaves extracts 6, 3, and 1.5 mg/ml were significantly different to control (p<0.05). Survival nauplii in fennel seeds extracts 1 and 0.5 mg/ml and liman leaves extracts 6 and 3 mg/ml were significantly different to control (p<0.05). The morphological abnormalities were exposed on coriander seeds extract 1 mg/ml, liman leaves extract 6 mg/ml, and pomegranate rinds extract 3 mg/ml. Nauplii with retarded development were presented on fennel seeds extracts 1 and 0.5 mg/ml, coriander seeds extracts 1 and 0.25 mg/ml, pomegranate rinds extracts 6, 3, and 1.5 mg/ml, and liman leaves extracts 3 and 1.5 mg/ml. Nauplii body length on plants extract showed no significantly difference compared to control.

Conclusion: Based on BSTST, fennel seed and pomegranate rind extracts had no teratogenic effect, kaempferia rhizomes act as larvicide its analog in mamalia as embryo lethal, while coriander seeds and liman leaves extracts potentially had teratogenic properties.


Keywords


Brine shrimp test, Teratogen, Fennel, Coriander, Pomegranate, Liman, Kaempferia.

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About this article

Title

BRINE SHRIMP TERATOGENIC SCREENING TEST ON SOME SELECTED PLANTS

Keywords

Brine shrimp test, Teratogen, Fennel, Coriander, Pomegranate, Liman, Kaempferia.

DOI

10.22159/ajpcr.2017.v10i8.18861

Date

01-08-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 8 August 2017 Page: 96-99

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

77 Views | 148 Downloads

Authors & Affiliations

Meti Widiya Lestari
Pharmacology-Clinical Pharmacy Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung - Indonesia.
Indonesia

Irda Fidrianny
Pharmaceutical Biology Research Group, School of Pharmacy, Bandung Institute of Technology, Bandung, Indonesia
Indonesia

Ayda T Yusuf
Biomedic of Animal Physiology and Development Research Group, School of Life Science and Technology, Bandung Institute of Technology, Indonesia
Indonesia

Andreanus A Soemardji
Department of Midwives, Tasikmalaya Polytechnic of Health Ministry of Health, Tasikmalaya, Bandung, Indonesia.
Indonesia


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