IN VITRO ANTIOXIDANT AND PHYTOCHEMICAL ANALYSIS OF DIFFERENT PARTS OF SIMANA LAGI APPLE ([MALUS DOMESTICA BORKH.] “SIMANA LAGI”) GROWN IN EAST JAVA-INDONESIA


Irda Fidrianny, Dhella Fitrani, Siti Kusmardiyani, Defri Rizaldy, Komar Ruslan

Abstract


 

 Objectives: The goals of this research were to observe antioxidant properties from different parts of Simana lagi apple ([Malus domestica Borkh.] “Simana lagi”) using two antioxidant testing methods which were 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2’-azino-bis(3-ethylbenzthiazoline-6- sulfonic acid (ABTS) and correlation of total phenolic and flavonoid content with their inhibitory concentration 50 (IC50) of DPPH and IC50 of ABTS.

Methods: Each sample was extracted by reflux using different polarity solvents. The extracts were evaporated using rotary evaporator. Antioxidant activities were tested using DPPH and ABTS assays, determination of total phenolic, and flavonoid content were carried out by ultraviolet-visible spectrophotometry and correlation with their IC50 of DPPH and IC50 of ABTS activities were analyzed by Pearson’s method.

Results: The ethanolic leaves extract (LV3) of Simana lagi apple also exposed the highest total phenolic content (TPC) (13.88 g gallic acid equivalents/100 g), while the highest total flavonoid content was presented by ethyl acetate extract (LV2) (7.21 g QE/100 g). The lowest IC50 of DPPH scavenging activity 0.19 μg/ml, and the lowest IC50 of ABTS scavenging activity 0.15 μg/ml was given by ethanolic flesh extract (FL3) of Simana lagi apple. There were significantly negative correlation between TPC in all different parts extracts of Simana lagi apple with their IC50 of DPPH and IC50 of ABTS.

Conclusions: All different parts extracts of Simana lagi apple were categorized as very strong antioxidant by DPPH and ABTS method (except n-hexane peels extract and n-hexane LV by DPPH method, and n-hexane FE by ABTS method). The major contributor in antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple by DPPH and ABTS methods were phenolic compounds. Antioxidant activities of peels, flesh and leaves extracts of Simana lagi apple showed linear results by DPPH and ABTS methods.


Keywords


Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid, Simana lagi apple, Malus domestica.

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About this article

Title

IN VITRO ANTIOXIDANT AND PHYTOCHEMICAL ANALYSIS OF DIFFERENT PARTS OF SIMANA LAGI APPLE ([MALUS DOMESTICA BORKH.] “SIMANA LAGI”) GROWN IN EAST JAVA-INDONESIA

Keywords

Antioxidant, 2,2-diphenyl-1-picrylhydrazyl, 2,2’-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid, Simana lagi apple, Malus domestica.

DOI

10.22159/ajpcr.2017.v10i11.20649

Date

01-11-2017

Additional Links

Manuscript Submission

Journal

Asian Journal of Pharmaceutical and Clinical Research
Vol 10 Issue 11 November 2017 Page: 41-45

Print ISSN

0974-2441

Online ISSN

2455-3891

Statistics

60 Views | 110 Downloads

Authors & Affiliations

Irda Fidrianny
School of Pharmacy Bandung Institute of Technology
Indonesia

Dhella Fitrani
School of Pharmacy Bandung Institute of Technology
Indonesia

Siti Kusmardiyani
School of Pharmacy Bandung Institute of Technology
Indonesia

Defri Rizaldy
School of Pharmacy Bandung Institute of Technology
Indonesia

Komar Ruslan
School of Pharmacy Bandung Institute of Technology
Indonesia


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