• Safriana
  • Rosidah Department of Pharmacology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Poppy Anjelisa Zaitun Hasibuan Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
  • Denny Satria Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.



Cytoprotective, Vernonia amygdalina Del, Ethanol Fraction, Hydrogen peroxide, Vero cells



 Objectives: The objective of this study is to assess the cytoprotective effect of ethanol fraction (EtF) of Vernonia amygdalina Del. leaves EtF against Vero cells which induced by hydrogen peroxide (H2O2).

Methods: Cytoprotective effects of EtF were analyzed by 3-(4,5-dimethylthiazolyl-2)2,5-dipheniltetrazolium bromide to Vero cells which induced by 0.8 mM H2O2, apoptosis was determined by flow cytometry, and reactive oxygen species (ROS) expression was analyzed by immunocytochemistry.

Results: EtF was showed the largest percentage viable cell (78.75±2.51%) at 50 μg/mL. In the analysis of apoptosis by flow cytometry was showed the percentage of viable cell count of 59.56% and EtF was decreased the expression of ROS.

Conclusion: EtF has cytoprotective activity toward Vero cells induced by 0.8 mM H2O2.


Download data is not yet available.


Diandana R. Knowing the Details of Cancer. 3rd ed. Yogyakarta: Katahati; 2009. p. 15, 22, 29-30.

Hawari D. Breast Cancer Psychoreligy Dimensions. Jakarta: FKUI Publisher; 2004. p. 12-5.

Sudiana IK. Molecular CancerPathobiology. Jakarta: Salemba; 2011. p. 1, 29-52, 71-5.

Sharma RA. Cancer Chemoprevention: A Clinical Reality. J R Soc Med 2000;93:518-20.

Clarkson PM, Thompson HS. Antioxidants: What role do they play in physical activity and health. J Clin Nutr Biochem 2000;72:637-46.

Navarro YJ, Zavala FL, Anandhan A, Wang F, Skotak M, Chandra N, et al. Antioxidant Gene Therapy Against Neuronal Cell Death. J Pharmacol Ther 2014;142:206-30.

Kosasih EN, Tony S, Hendro H. The Role of Antioxidants on Elderly. Jakarta: National Center for The Geriatrics Problems; 2006. p. 67-78.

Giorgio PP. Flavonoids as antioxidants. J Nat Prod 2000;63:1035-42.

Montagnon BJ, Fanget B, Nicolas AJ. the large scale cultivation of vero cells in micro carrier culture for virus vaccine production. preliminary results for killed poliovirus vaccine. Dev Biol Standard 1981;47:55-64.

Goncalves EM, Ventura CA, Yano T, Macedo ML, Ganeri SC. Morphological and growth alterations in vero cells transformed by cysplatin. Cell Biol Int 2006;30:485-94.

Anggraeni R, Hadisahputra S, Silalahi J, Satria D. Combinational effects of ethylacetate extract of Zanthoxylum acanthopodium DC. With doxorubicin on T47D breast cancer cells. Int J Pharmtech Res 2014;6:2032-5.

Hasibuan PA, Rosidah. Combination effect of ethylacetate extract of Plectranthus amboinicus (Lour.) Spreng. with Doxorubicin againts Hela Cell Lines. Int J Pharm Chem Res 2016;8:357-60.

Satria D, Silalahi J, Haro G, Ilyas S, Hasibuan PA. Antioxidant and antiproliferative activities of an ethylacetate fraction of picria fel-terrae Lour. Herbs. Asian Pac J Cancer Prev 2017;18:399-403.

Hartati E. Cytotoxic Effects of African leaf of Ethanol Extract (Vernonia amygdalina Del.) on hela cells and vero cells. Essay Medan: Faculty of Pharmacy. North Sumatera University; 2015.

Sumardika IW, Jawi IM. Water extract of sweet potato leaf improved lipid profile and blood SOD content of rats with high cholesterol diet. Med Sci J 2012;43:67.15.



How to Cite

Safriana, Rosidah, P. A. Z. Hasibuan, and D. Satria. “CYTOPROTECTIVE EFFECT OF ETHANOL FRACTION OF VERNONIA AMYGDALINA DEL. LEAVES AGAINST THE VERO CELLS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 13, Apr. 2018, pp. 214-6, doi:10.22159/ajpcr.2018.v11s1.26610.



Original Article(s)

Most read articles by the same author(s)

1 2 > >>