CYTOPROTECTIVE EFFECT OF ETHANOL FRACTION OF VERNONIA AMYGDALINA DEL. LEAVES AGAINST THE VERO CELLS
Keywords:Cytoprotective, Vernonia amygdalina Del, Ethanol Fraction, Hydrogen peroxide, Vero cells
Â Objectives: The objective of this study is to assess the cytoprotective effect of ethanol fraction (EtF) of Vernonia amygdalina Del. leaves EtF against Vero cells which induced by hydrogen peroxide (H2O2).
Methods: Cytoprotective effects of EtF were analyzed by 3-(4,5-dimethylthiazolyl-2)2,5-dipheniltetrazolium bromide to Vero cells which induced by 0.8 mM H2O2, apoptosis was determined by flow cytometry, and reactive oxygen species (ROS) expression was analyzed by immunocytochemistry.
Results: EtF was showed the largest percentage viable cell (78.75Â±2.51%) at 50 Î¼g/mL. In the analysis of apoptosis by flow cytometry was showed the percentage of viable cell count of 59.56% and EtF was decreased the expression of ROS.
Conclusion: EtF has cytoprotective activity toward Vero cells induced by 0.8 mM H2O2.
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